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The impact of expedited third trimester viral load testing on the proportion of vaginal deliveries in HIVpositive pregnant women in the Dominican Republic

DOI: 10.31038/IGOJ.2022523

Abstract

Objective: Advances in HIV treatment have led to a significant decrease in vertical transmission. Lack of adequate viral load testing capabilities inhibited the ability to follow national and international guidelines for obstetric care in the Dominican Republic (DR). The objective of this study was to determine if expedited third trimester viral load testing in HIV-positive pregnant women led to an increase in vaginal deliveries at a clinic in the DR, thus demonstrating the ability to follow national guidelines for obstetric delivery of HIV-positive women on antiretroviral therapy (ART).

Study Design: This study enrolled pregnant HIV-positive patients at a clinic in the DR October 2014-July 2015. Viral load testing was performed 34-36-weeks gestation and results were available within 48 hours. Demographic information, clinical factors, and obstetric outcomes were collected and compared to patients in a retrospective cohort, who delivered January 2012-December 2012 when expedited viral load testing was unavailable.

Results: Of the 20 women in the study, 17 (85%) had viral loads <1000 and seven women (35%) delivered vaginally. In the comparison retrospective cohort, of 41 women, three women (7%) had vaginal deliveries. Comparing the two groups, there was a statistically significant increase in vaginal deliveries from 7% to 35% (p=0.02) after expedited viral load testing was made available. All infants born in the study were HIV-negative.

Conclusion: The study with expedited viral load testing available had an increased number of vaginal deliveries of HIV-positive women on ART. The majority of these patients were on ART with HIV viral loads <1000, and access to viral load results allowed for providers and patients to plan for vaginal deliveries as indicated by national guidelines. These results reinforce the importance of access to timely viral load testing for pregnant women with HIV and support previous research demonstrating no increase in vertical transmission from mother to infant during vaginal delivery.

Keywords

HIV, Vertical Transmission, Viral Load, Dominican Republic, Caribbean

Introduction

The Caribbean region has the second-highest prevalence of human immunodeficiency virus (HIV) in the world after sub-Saharan Africa, with the Dominican Republic (DR) and Haiti accounting for nearly two-thirds of all new HIV cases in this area [1]. Currently, approximately 72,000 individuals are living with HIV in the DR, yielding a prevalence of 0.9% in adults [2]. Although much progress has been made, mother-to-child (i.e., vertical) transmission of HIV remains significant, with approximately 1,300 children below the age of 15 living with HIV in the DR [2,3].

Advances in HIV treatment and monitoring are changing the landscape of vertical transmission of this disease. Current guidelines from the United States and DR recommend that women infected  with HIV receive antiretroviral therapy (ART) during pregnancy, because ART significantly decreases vertical transmission rates [4,5]. Before ART, Cesarean sections (C-section) were recommended for all HIV-positive women to decrease the risk of HIV transmission to the infant during delivery [6]. However, C-section carries significant risks including wound infection, infant respiratory problems, and a higher rate of maternal complications with future pregnancies (e.g., uterine rupture, placenta previa, placenta accreta, and bowel and bladder injury) [7]. As access to ART became widespread, studies demonstrated that pregnant patients on ART achieve a viral load  low enough to decrease the risk of vertical transmission such that C-section and vaginal delivery carry the same vertical transmission rate [4]. Additionally, the DR has one of the highest maternal mortality rates in the region and the second highest C-section rate   in Latin America [8,9]. International and Dominican guidelines now recommend that HIV-positive pregnant women who are on ART by the third trimester and meet certain criteria (e.g., HIV viral load less than 1000; negative syphilis, Hepatitis B, and Hepatitis C testing; fewer than two prior C-sections; no C-section in the past two years) should consider vaginal delivery in consultation with their obstetrician [4,5].

At the time of the study described herein, the DR had one viral load analyzer serving the entire country’s population and was unable to support the testing demands. Thus, most HIV patients were either not monitored for viral load every six months, as recommended, or had their results returned months after testing, thereby decreasing clinical utility.

Located in the town of La Romana in the south-eastern region of the DR, Clínica de Familia La Romana (CFLR) is a non-profit primary care clinic that provides ambulatory services and houses an HIV clinic, in addition to providing care to HIV-positive pregnant women and their infants through a vertical transmission program.  At the  time this study was initiated, all HIV-positive pregnant women receiving care at CFLR were scheduled for delivery via C-section at 38 weeks gestation. Although national and international guidelines recommend considering vaginal delivery for women on ART with viral loads under 1000 at 34-36 weeks gestation, providers at CFLR were unable to follow these guidelines, because the in-country viral load testing program could not provide the required results in a timely fashion.

This study aimed to determine whether expedited viral load testing would be associated with an increase in planned vaginal deliveries in HIV-positive pregnant women in a vertical transmission program in the south-eastern DR (i.e., at CFLR).

Methods

This pilot study of HIV-positive pregnant women enrolled in a vertical transmission program at a primary care clinic, CFLR, and at    its affiliated adolescent reproductive health clinic in La Romana in the south-eastern DR was performed from October 2014 through July 2015. All HIV-positive pregnant patients who were less than 36 weeks gestation and who were enrolled in CFLR’s vertical transmission program were recruited and enrolled after providing written informed consent.

Demographic, clinical, and laboratory data were collected from patient medical records including the date of HIV diagnosis, current ART treatment and adherence, obstetric clinical history, expected date of delivery, planned and actual mode of delivery, maternal and infant outcomes, infant treatment regimen, maternal CD4 count, maternal complete blood count, and infant HIV PCR results at six weeks    and six months post-partum. The mode of delivery was categorized as emergency C-section, elective C-section, or vaginal. Prior to initiation of the study, clinic physicians and staff were already aware of national guidelines for delivery options for HIV-positive pregnant women, so no additional education on these topics was necessary.

Whole blood specimens were collected from patients at 34-36 weeks gestation by trained CFLR phlebotomists. The samples were prepared and shipped overnight via FedEx to the New York Presbyterian/Columbia University Medical Center (CUMC) Clinical Microbiology Laboratory in accordance with specifications for the COBAS® TaqMan® HIV-1 Test, v2.0 and United States Category B infectious shipping regulations. Viral load testing was performed using the COBAS® TaqMan® HIV-1 Test, v2.0 in the CUMC Clinical Microbiology Laboratory and the results were uploaded within 48 hours from receiving the sample at CUMC onto a secure server for remote viewing by research staff at CFLR. Research personnel were available for questions and comments from staff and patients throughout the duration of the study. Research staff provided the HIV viral load results to the patient’s medical team at CFLR, who independently utilized the results in the patient’s care management and delivery planning and included the results in the referral paperwork that each patient brought with them to the hospital at  the time of delivery. The obstetricians at CFLR were often the same providers performing the deliveries at the hospital. Data on the mode of delivery and maternal and infant outcomes were later extracted from the patient’s medical record.

The mode of delivery and maternal and infant outcomes were compared to a historical cohort comprised of the clinic’s vertical transmission program patients from a prior year during which timely viral load testing was not available. The historical cohort included obstetric HIV-positive patients cared for at CFLR and who delivered between January 1 and December 31, 2012 for whom HIV viral load results were not available during late pregnancy. Of the 55 patients who delivered in this time period, six patients were excluded due to insufficient recorded data and eight were excluded due to diagnosis with HIV at the time of their delivery.

The study protocol was approved by the Institutional Review Board of CUMC and by the “Consejo Nacional de Bioética en Salud” (CONABIOS), the ethical review board in the Dominican Republic.

Statistical Analysis

Analysis of the retrospective cohort data from 2012 was used to calculate a clinically meaningful vaginal delivery difference for the pilot group. Since the retrospective data from 2012 did not contain sufficient information (i.e. third trimester HIV viral load) to posit which women would have met clinical criteria for a vaginal delivery, we estimated that 41% of women in the retrospective cohort would have met clinical criteria for a vaginal delivery, given that they were

(1) receiving appropriate suppressive ART (and would thus likely have a viral load less than 1000) and (2) had a parity < 2 (as a proxy for those who were less likely to have had a previous C-section, given a 50% C-section rate in the DR and since C-sections are the major exclusion criteria for vaginal deliveries). Given variability in patient and provider preference of delivery mode, we determined that a proportion of vaginal deliveries of 25% in the pilot study cohort (relative to 7% in the retrospective cohort) would reflect a clinically meaningful difference.

Descriptive statistics were used to characterize baseline characteristics, HIV viral load testing, mode of delivery, and infant HIV PCR test results. Fisher’s exact test was performed to test for differences in the mode of delivery between the pilot study cohort and the retrospective cohort. All analyses employed two-tailed testing with a threshold of p<0.05 considered statistically significant. Data were analyzed using OpenEpi.

Results

Twenty women were recruited into the pilot study cohort during the nine-month study period in 2014-2015 and 41 women were included in the retrospective (2012) cohort. Mean (SD) age of women was 21.2 (4.0) years in the pilot cohort and 25.7 (6.3) years in the retrospective cohort (Table 1). HIV viral load testing was successfully completed at CUMC on all 20 patients in the pilot cohort at 34-36 weeks gestation, whereas in the retrospective cohort, one patient had an HIV viral load performed at 34-36 weeks gestation (Table 1).

Table 1: Baseline characteristics, HIV viral load testing, mode of delivery, and infant HIV PCR test results for participants in the 2012 retrospective cohort and 2014 pilot cohort of HIV-positive pregnant women.

 

2012 Retrospective cohort (n=41)

2014 Pilot study cohort (n=20)

Maternal characteristics

Mean (SD)

 

Age (years)

25.7 (6.3)

21.2 (4.0)

     
 

N (%)

 

HIV viral load testing performed at 34 to 36 weeks gestation

1 (2%)

20 (100%)

Mode of delivery

   

Vaginal

3 (7%)

7 (35%)

Cesarean section

38 (93%)

13 (65%)

Infant Characteristics    

HIV PCR result at 6 weeks of agea

   

Negative

39 (97.5%)

21 (100%)

Positive

0 (0%)

0 (0%)

Indeterminate

1 (2.5%)

0 (0%)

HIV PCR result at 6 months of ageb

   

Negative

7 (87.5%)

20 (100%)

Positive

1 (12.5%)

0 (0%)

Indeterminate

0 (0%)

0 (0%)

aAt six weeks, there were N=21 infants in the pilot study cohort (one set of twins) and there were N=40 infants in the retrospective cohort due to loss to follow-up.
bAt six months, there were N=20 infants in the pilot study cohort (one infant passed away due to unknown reasons) and N=8 infants in the retrospective cohort (the remainder did not have 6-month HIV PCR results recorded in their clinical charts).

Of the women in the pilot cohort, seven (35%) delivered vaginally and 13 (65%) delivered by C-section. In the retrospective cohort, three (7%) delivered vaginally and 38 (93%) delivered by C-section. In the pilot cohort, 17 (85%) had viral loads less than 1000 copies per mL (meeting viral load criteria for a vaginal delivery), and of those 17 women, six (35%) delivered vaginally (Table 2). Of the three women with elevated HIV viral loads, two had C-sections and the third woman arrived at the hospital in labor with a precipitous vaginal delivery. The deliveries were otherwise uncomplicated.

Table 2: 2014 pilot study cohort viral load testing and mode of delivery (N=20)

 

HIV viral load <1000 copies/ml N=17 (85%)

HIV viral load ≥1000 copies/ml N=3 (15%)

 

N (%)

 
Vaginal delivery

6 (35%)

1 (33%)

Cesarean section

11 (65%)

2 (67%)

Compared to the retrospective cohort, the pilot study cohort where viral load testing and results were made available prior to     38 weeks gestation had a significantly higher proportion of vaginal deliveries (7% vs. 35%, p=0.02). The observed proportion of vaginal deliveries in the pilot study cohort (35%) was higher than the predicted proportion (25%) from pre-study calculated parameters. Although target enrollment was not achieved, the post-hoc power calculation using 20 participants revealed a power of 81%.

At six weeks of age, all 21 infants in the pilot study cohort (including one set of twins)  had  negative  PCR  HIV  testing  and 20 infants had negative PCR testing at six months of  age  (one infant passed away due to unknown reasons before the six-month time point) (Table 1). In the retrospective cohort, at six weeks of  age, 39 infants had negative PCR HIV testing, one infant had an indeterminate result, and one infant did not have a result due to loss to follow-up. There are limited data available for the retrospective cohort infants at six months of age; however, the infant with the initial indeterminate result had a positive result at six months of age. This infant was born via C-section to a mother who was diagnosed with HIV during pregnancy and started on antiretroviral therapy at the 28-weeks gestation.

Discussion

Overall, the availability of expedited viral load testing and access to results was associated with an increased likelihood of vaginal deliveries in this vertical transmission program in the DR. Additionally, there was no associated increase in vertical transmission of HIV, which is consistent with findings of other studies [10].

Nonetheless, our study had several limitations. As discussed previously, in the power calculation, there was difficulty determining the expected proportion of vaginal deliveries in the pilot study cohort given limited data from the retrospective cohort. The sample size for the pilot study cohort (N=20) was significantly smaller than the prior cohort (N=41) due to the implementation of a prenatal vertical transmission program at the local public hospital, where many women deliver their infants, absorbing much of CFLR’s patient load during the time the study was completed. Additionally, study time was decreased from 12 months to 9 months due to personnel limitations. Chart abstraction did not provide clear data on indications for C-section at the hospital, as hospital notes were not available. Due to patient loss to follow up and limitations in chart abstraction, there was missing data for infant HIV PCR results at six months of age. Finally, the statistical analysis performed to test for differences in delivery mode between the retrospective and pilot study cohort did not control for any additional variables that might have differed between the groups, due to limitations in data abstracted from the clinical charts.

Despite these limitations, having viral load testing performed and the results available in an expedited fashion provided women and their care team with the option of a vaginal delivery, in keeping with national and World Health Organization guidelines for HIV vertical transmission programs. Although the model used in this study (i.e., expedited shipping to an academic medical center in the United States) is expensive, findings from this study demonstrate the benefits of improved access to viral load testing equipment to evaluate HIV-positive patients, especially when it can dramatically alter management and avoid unnecessary abdominal surgery. In December of 2016, CFLR received a donation of a GeneXpert instrument for HIV viral load testing, in part, as a result of these study results. The clinic is now able to provide viral load testing on site, greatly reducing the time it takes to get results, both for pregnant women and for other HIV-positive patients. Although CFLR now has these capabilities, much of the DR still does not have access to timely viral load testing. As demonstrated by this study, increased access to and more efficient HIV viral load testing, analysis, and distribution of results could help to reduce the number of unnecessary C-sections in pregnant women with HIV in the DR.

Acknowledgements

We thank Jane Netterwald for expert technical assistance. Funding  was  provided  by  New  York Presbyterian/Columbia University Medical Center (CUMC) Clinical Microbiology Laboratory.

References

  1. Joint United Nations Programme on HIV/AIDS. Global AIDS Update 2018: Miles to go: The response to HIV in the Geneva: UNAIDS; 2018. [Crossref]
  2. Joint United Nations Programme on HIV/AIDS. Country Fact Sheets: Dominican Republic 2019. Geneva: UNAIDS; 2020.: [Crossref]
  3. Lorenzo O, Beck-Sagué CM,  Bautista-Soriano  C,  Halpern  M,  Roman-Poueriet J, Henderson N, et al. Progress towards elimination of HIV mother-to-child transmission in the Dominican Republic from 1999 to 2011. Infect Dis Obstet Gynecol. 2012;2012:543916.
  4. Panel on Treatment of Pregnant Women with HIV Infection and Prevention of Perinatal Transmission. Recommendations for Use of Antiretroviral Drugs in Transmission in the United Washington, D.C.: U.S. Department of Health and Human Services; 2020. Available from: [Crossref].
  5. Ministerio de Salud Pública. “Guía Práctica Clínica de las Infecciones de Transmisión Sexual,” Dominican Republic, 2013.
  6. Azria E, Kane A, Tsatsaris V, Schmitz T, Launay O, Goffinet Term labor management and outcomes in treated HIV-infected women without contraindications to vaginal delivery and matched controls. Int J Gynaecol Obstet. 2010;111(2):161-164. doi:10.1016/j.ijgo.2010.05.023.
  7. American College of Obstetricians and Gynecologists (ACOG) Committee on Obstetric Practice. ACOG Committee Opinion Number 559: Cesarean Delivery on Maternal Request. Obstet Gynecol. 2013 Apr;121(4):904-7. doi: 10.1097/01. AOG.0000428647.67925.d3.
  8. Gibbons L, Belizán JM, Lauer JA, Betrán AP, Merialdi M, Althabe World Health Report: The Global Numbers and Costs of Additionally Needed and Unnecessary Caesarean Sections Performed per Year: Overuse as a Barrier to Universal Coverage, Background Paper, 30. Geneva: World Health Organization; 2010. Available from: https://www.researchgate.net/publication/265064468_The_Global_Numbers_ and_Costs_of_Additionally_Needed_and_Unnecessary_Caesarean_Sections_ Performed_per_Year_Overuse_as_a_Barrier_to_Universal_Coverage_HEALTH_ SYSTEMS_FINANCING.
  9. World “Maternal mortality ratio (modeled estimate, per 100,000 live births),” Washington, D.C.: World Bank; 2010. Available from: https://data.worldbank. org/indicator/SH.STA.MMRT?order=wbapi_data_value_2010+wbapi_data_ value+wbapi_data_value-first&sort=asc.
  10. Garcia PM, Kalish LA, Pitt J, Minkoff H, Quinn TC, Burchett SK, et al(. Maternal levels of plasma human immunodeficiency virus type 1 RNA and the risk of perinatal transmission. Women and Infants Transmission Study N Engl J Med. 1999 Aug 5;341:6:394-402.

A Reappraisal of Einstein’s Light-Quantum Hypothesis

DOI: 10.31038/NAMS.2023611

Abstract

Einstein published his light-quantum hypothesis (LH) 1905, suggesting that a light ray transmits discrete energy quanta h.fi, emitted at speed c from their source, where h refers to Planck’s constant – and fi to the frequency of a ray of light Lx in accordance with the wave equation  c=λi.fi – with λi  indicating the wavelength of Lx. Now, let fv and fr stand for the frequency of violet and red light, respectively, assuming that  fv=2.fr .  Following these premises, a source of light Qv emitting h.fv light-quanta would transmit an energy amounting to (h.fv.fv) per second, whereas a source Qr, releasing h.fr light-quanta would only emit an energy equivalent to (h.fr.fr). In other words: The LH implies that the energy a ray of light transports per time-unit corresponds to the square of its frequency. This stands at odds with the experimentally established view that the energy carried by different light-rays per time-unit is linearly proportional to their (respective) frequency.

Introduction

Einstein introduced 1905 the light-quantum-hypothesis (LH) in his paper «Über einen die Erzeugung und Verwandlung des Lichtes betreffenden heuristischen Gesichtspunkt» [On a heuristic point of view concerning the production and transformation of light]. With the help oft he LH he aimed to interpret the photoelectric effect – as well as other phenomena related to it – in a consistent, straightforward way.

In the present paper, I shall

  1. Consider the LH as a purely theoretical system, based on a number of explicit and implicit postulates;
  2. Check whether the postulates underlying the LH are self-consistent and mutually compatible;
  3. Analyse certain implications of the LH, and compare them with the generally accepted interpretation of the experimental findings.

*An earlier version of this paper was published in GALILEAN ELECTRODYNAMICS, Volume 30, Number 5, September/October 2019, p. 95-97.

Explicit and Implicit Postulates Underlying the LH

P1:          Let sources of light Qx emit homogenous, mono-frequent rays of light Lx.

P2: Let the wave-equation

(1) c=λi . fi

be valid for every light-ray Li emitted by a source Qi, with λi referring to the wavelength of a ray of light Li, and fi to the frequency of Li.

P3: Let Lv be of a higher frequency than Lr, i.e. fv > fr, with ‘v` standing for “violet“, ‘r’ for red.

P4: Let every source of light Qi which emits rays of light of a given frequency fi, emit these rays as bundles of light-quanta or photons Phi having the energy h.fi, i.e.

(2) Ei=h.fi,

where ‘h‘ refers to Planck’s constant (elementary quantum of action), and ‘fi’ to the frequency.

P5: Let the light-quanta (=energy quanta, photon) Phi, which are emitted by Qi, move through space as indivisible entities, and let them be absorbed as such by adequate targets.

P6: Let each light-quantum Phi move with speed c  in relation to its source Qi – if mutual conditions of rest are given between source, receiver, and medium (insofar a medium needs to be taken into account).

A short explanation of the foregoing postulates reads as follows:

Postulate P1 is seldom assumed explicitly. Einstein, in his introductory remarks, observed that he would consider the energy transmitted by a light-ray emitted by a «Lichtpunkt» (i.e. a very small source of light). With respect to the following analysis, I’ll assume the existence of « quasi-linear » rays of light, i.e. tiny bundles of light the cross-sections of which are so small that only one light-quantum per unit of time can hit and perforate an adequately placed perpendicular plane. Said in other terms, and in accordance with Einstein: I’ll consider rays of light in which light-quanta move in a linear row.

Postulate P2 is very rarely formulated explicitly. Nevertheless, it is implicitly taken for granted as soon as one:

(a) alludes to the frequency fi of a given light-ray, and distinguishes rays of light of different frequencies;

(b) introduces c as the constant speed of every light-ray Li in relation (i) to its source, (ii) to the medium (insofar a medium has to be considered), (iii) the receptor, and (iv) the « external observers » – albeit on the premises that source, medium, receptor, and « external observers » are at rest with respect to each other;

(c) assumes that the fronts of the rays of light – e.g. of violet and red light – proceed from their respective sources at equal speeds c.

Postulate P3 is generally accepted and needs no further comment.

Postulates P4 and P5 comprise the main, central, statements of Einstein’s LH [1]. Strictly speaking, P4 and P5 contain three separate assertions, concerning:

(i) the emission of light-quanta (see: P4);

(ii) the propagation through space of indivisible light-quanta (see: P5), and

(iii) the absorption of indivisible light-quanta (see: P5).

However, since I am dealing in the present paper only with the emission and the propagation of light-quanta, I have subsumed propagation and absorption to the same postulate P5.

Postulate P6 is seldom stated explicitly. In a configuration stating mutual rest of source, receiver, medium (insofar a medium needs to be considered), and observers – P6 is self-evident. Furthermore, P6 can be derived from P2 in conjunction with P4 and P5 [2-5].

Discussion of the LH

We shall start by considering the postulates P1 – P6 independently of one another: As far as I can see, there are no ambiguous demands being made, and nothing otherwise untenable can be discerned. Thus, every single postulate  P1 – P6, taken on its own, is free from contradictions [6,7].

We must now look at several combinations of these postulates:

(i) It follows from P3 and P4 that Lv-photons consist of a larger amount of energy than Lr-photons. In other terms:

(Ev=h.fv) > (Er=h.fr).

(ii) According to P4 in conjunction with P5, the light-quanta spread from their respective sources as indivisible entities, in their respective frequencies fv and fr – which is a constitutive factor of the light-rays we are considering.

(iii) With regard to P2, it follows that every frequency fi is univocally correlated to a corresponding wavelength λi, so that any kind of event belonging to Li (e.g. its wave-peaks) will be repeated with the corresponding frequency fi at any well-defined point along the path of Li that continues to move forth with speed c.

(iv) According to P4, source Qv emits light-quanta of energy h.fv, whereas a source Qr will release light-quanta of energy h.r.

(v) Now, following P3, ‘fv’ refers to the frequency of violet light-rays and ‘fr’ to the frequency of red light-rays, with fv > fr. On the basis of P2, we will then, reciprocally, have to infer that λv < λr.

This assertion implies that source Qv will not only emit light-quanta Phv which excel in energy the light-quanta Phr emitted by source Qr by the factor (fv – fr), but also that source Qv is due to propagate its light-quanta Phv with a frequency which surpasses the frequency with which source Qr releases its light-quanta Phr by the same factor (fv – fr).

In other words: On the basis of P1 – P6, we are compelled to deduce that if we – for instance – compare a ray of light Lv of wave-length λv=4000 nm with a ray of light Lr of wavelength λr=8000 nm, the former one (i.e. Lv) must transmit four times (and not twice) as much energy per unit of time than the latter (i.e. Lr).

It is hard to see, how this unexpected implication could be avoided: The frequency fi is a firmly bound variable of equation (1) [c=λi.fi]. – As soon as we use the symbol ‘fi‘ to point at the energy of a light-quant h.fi, we are forced to accept that these quanta are linked to equation (1) and are, therefore, emitted fi times per unit of time.

(vi) Einstein believed that the amount of energy contained in a single light-quantum  h.fi is linearly proportional to ist frequency, i.e. to the fi. However, on the basis of his premises – i.e. of his postulates P1 – P6 – we are logically compelled to infer that Einstein’s premises do not support what Einstein believed to be true, but -on the contrary – supported the erroneous assumption that the amount of energy contained in a single light-quantum h.fi is proportional to the square its frequency.

(vii) In 1916 [8]: Millikan pointed out: “The hypothesis [i.e. Einstein’s LH] was apparently made solely because it furnished a ready explanation that when an electron is thrown out of a metal by ultra-violet light or X-rays it is independent of the intensity of the light while it depends on its frequency .“ However, if one correctly argues based on the experimental findings established by Millikan – that the amount of energy being transported by a ray of light Li per unit of time is linearly proportional to its frequency – one is compelled to dismiss as erroneous the core of Einstein’s theoretical premises and his reasonings based on them. In short: Einstein’s theoretical premises and his reasonings based on them stand at odds with his belief and with the experimental findings established by Millikan.

Conclusion

Einstein’s light quantum hypothesis (LH) prescribes that every ray of light Li transports and transmits discrete energy-light-quanta of magnitude h.fi.If every source of light Qi emitted discrete light-quanta of a specific, frequency-dependent magnitude h.fi, it would also have to release these energy-quanta with the corresponding frequency fi. This would, in turn, imply that the energy Qi emitted per unit of time with a ray of light Li, had to be proportional to the square of its frequency, i.e. the amount of the propagated and transmitted energy would have to be equivalent to fi.(h.fi) per unit of time.However, if we hold to the generally acknowledged – and by Millikan [9] experimentally established – view that every ray of light transports and transmits an amount of energy per unit of time which is linearly proportional to its frequency, Einstein’s premises and his reasonings cannot be maintained.

References

  1. Einstein A (1905) Über einen die Erzeugung und Verwandlung des Lichtes betreffenden heuristischen Gesichtspunkt. Annalen der Physik. 17: 132-148.
  2. Einstein A (1905) Ist die Trägheit eines Körpers von seinem Energieinhalt abhängig ? Annalen der Physik. 18: 639-641.
  3. Einstein A (1906) Zur Theorie der Lichterzeugung und Lichtabsorption. Annalen der Physik 20: 199- 206.
  4. Einstein A (1909) Zum gegenwärtigen Stand des Strahlungsproblems. Physikalische Zeitschrift 10: 185-193.
  5. Gut B (1996) Einstein’s Lightquantum Hypothesis. Analysis, Implications-and an Alternative. The Toth-Maatian Review 13: 5925-5929.
  6. Kaase H, Scrick F (2004) Optische Strahlung und ihre Messung. Bergmann, Schaefer. Lehrbuch der Experimentalphysik. De Gruyter 633-668.
  7. Kane JW, Sternheim MM (1988) Physics. John Wiley. 3rd ed.
  8. Millikan RA (1916) Quantenbeziehungen beim photoelektrischen Effekt. Physikalische Zeitschrift 17: 217-221.
  9. Millikan RA (1916) A direct photoelectric determination of Planck’s ‘h’. Physical Review 7: 355-388.

Empowering Critical Thinking among Young Students: Exploring Messaging about Bullying

DOI: 10.31038/PSYJ.2023522

Abstract

The study reported here investigates the response to ideas about bullying, these ideas emerging from the interaction of a young, eight-year old researcher with an artificial intelligence system (Idea Coach). The program permits the researcher to suggest the topic (bullying), uses the AI Idea Coach to create sets of 30 questions about bullying, requires to the research to select four questions, lets the AI suggest 15 answers to each question, requiring the researcher to select four of the answers for each question. The program then combined the answers (elements) into small combinations, comprising 2-4 elements, each respondent of the group (110 individuals, ages 15-26) evaluating a totally unique set of 24 combinations (vignettes). Deconstruction of the vignettes based upon the response ‘makes sense’ reveal three different mind-sets, focusing on WHO is a likely bully, How to STOP a likely bully and less clearly but still emerging, the reason WHY person is a bully, respectively. The approach shows the feasibility of AI as an enhancer of critical and creative thinking, empowering students as young as eight years able to begin doing high-level, original scientific research, in a systematized, programmed fashion.

Introduction

This paper is part of a series of research studies conducted by young students, enrolled in elementary and in middle school, with the dual goal of teaching students how to think critically while at the same time working with them as researchers to explore issues of social relevance. A glance at the literature reveals that virtually all research is executed by professionals, occasionally junior professionals such as graduate students, but far more often by young and middle-aged professionals, following the structure of academic research. Even when the topic involves the experience of young people, such experience is analyzed through the ‘trained’, perhaps biased eye of the professional, who brings along a career of studies. The study of the experience follows the time-hallowed practices of the scientific method, although the ‘immediacy’ of the experience cannot be tapped because the researcher is not a young person.

Almost 80 years ago, the radio and television personality Art Linkletter starred in a show called House Party, starting in 1945 and ending in 1969. As described by Wikipedia. The host would begin a conversation by posing a question about life topics to a child, who usually responds with their own innocent, often comedic perspectives on the various topics…. In the show’s first inception, it would sometimes flash back to the 1950s and 1960s show Art Linkletter’s House Party, with Cosby joined onstage by Art Linkletter, that show’s host, to introduce the vintage clips. It would show kids (of the time), their same comedic reactions to whatever Linkletter would ask or say to them…

The foregoing quote suggests that we may learn a great deal about the way children think about the world, although it is clear that a great deal of this learning is second-hand, with the researcher observing the behavior, and commenting on it. In recent years authors Deitel, Moskowitz and Rappaport have collaborated to create a system whereby anyone can be a researcher, at least for the type of research known as Mind Genomics. The idea behind Mind Genomics is that by showing people combinations of ideas and measuring their response, one can learn what specific aspects of these ideas drive the behavior, viz., the aspects to which people pay attention. Rather than asking people to say what is important to them about a topic (viz., open ended question), or ask people to rate importance of different ideas, one idea at a time, Mind Genomics assumes that people will find it more ‘natural’ to respond to combinations of ideas. The rationale for this assumption is that in the world of everyday experience people evaluate combinations of features, rather than one single feature at a time.

Bullying

The topic of bullying is becoming increasingly important [1-3]. It may be that the availability of video technology on smartphones to record one’s bullying efforts has become a stimuli to become a hero. Bullying on the internet has been shown to be responsible for teen and child suicides [4,5]. Those are just the most serious cases.

The emerging science of Mind Genomics may help us understand what young people think is important. The study reported here is unique because it was set up by young researchers, in elementary schools (3rd grade; Ciara) and in middle school (8th grade; Cledwin). The study objective was to explore aspects of bullying from the point of view of the younger researcher, using Mind Genomics augmented with AI, in the form of Idea Coach. Idea Coach helped the researcher both learn about the issues involved in bullying, and to take an active role providing new-to-the-world knowledge about these issues.

The Mind Genomics approach has been previously discussed in a variety of papers [6,7]. Three previous studies have appeared featuring the efforts of these young researchers [8,9]. The objective of the studies is to deal with a serious topic, framed not so much by an experienced adult researcher as is usually the case but rather a research effort framed by a young mind. Often the types of questions intriguing the young researcher may well differ from the questions intriguing an older researcher. We almost never heard from the young researcher, and from young respondents. The study reported here and its companion studies allow us a peek into the mind of the younger researcher, and the response to test stimuli by respondents of the appropriate age.

Method

Mind Genomics studies follow a now-standard approach, using templated inputs and automated, rapid analyses. The almost automated approach exemplifies the vision of author Moskowitz to create a system which allows anyone to become a researcher (democratization), coupled with the goal of accelerated knowledge development (efficiency), and with the vision of creating large-scale databases of aspects of ordinary life, doing so in a simple, inexpensive, iterative, and world-wide manner (industrial-scale knowledge development). The Mind Genomics program itself is called BimiLeap, short for Big Mind Learning App. The program can be accessed by anyone with access to the Internet, at www.BimiLeap.com.

Step 1 – Identify the Specific Topic

This sounds simple, and eventually is simple. The novice researcher often thinks in generalities, not in specifics.

Step 2 – Create Four Questions Regarding the Topic of the Study

The respondent is allowed to choose any questions, as long s the questions tell a story about the topic. It is at this point that Mind Genomics proceeds to structure the way the researcher approaches the problem. The first encounter with requiring the researcher to ask a question can be frightening, and certainly off-putting. Most novice researchers freeze up at this point, perhaps shocked by the directness of the question. The most common initial reaction at this point is a sense of discomfort, and in some cases that discomfort simply ends the effort, with the researcher.

For the researchers who cannot think of questions, there is the associated AI feature, Idea Coach. The researcher simply writes a short paragraph, really 1-3 sentences, and Idea Coach returns with 10-30 questions. Idea Coach can be invoked several times, either with the same paragraph or with different paragraphs. Each time Idea Coach will return with a set of somewhat different questions. Table 1 shows a run of Idea Coach, prompted by the researcher request ‘Help me create questions about bullying.’

Table 1: Thirty questions emerging from Idea Coach, based upon the request to ‘tell me some questions to ask about bullying in school’.

tab 1

 

Once the researcher selects the four questions, the next step requires the research to provide four answers to each question, specifically phrases which paint word pictures. Once again the researcher can either come up with answers, and/or work with the Idea Coach. For each ‘run’ of Idea Coach, comprising answers to a question, Idea Coach emerges with approximately 15 phrases which answer the question. This step in the process is much easier, simply because many people find it easier to answer questions than to pose questions. Answering questions relies on memory and experience. Posing questions relies more on critical thought because the researcher is going into a void, rather than filling the void under the direction of a question.

The process in Mind Genomics has been simplified, allowing the researcher to write the question to describe the topic, use Idea Coach to present 30 questions (and repeat as desired), select four questions, edit those questions when desired, and finally put in answers and have Idea Coach fill in the missing answers. In all cases the researcher is free to override the Idea Coach effort, whether overriding consists of editing the question/answer or even providing a new question/element based upon one’s insight.

Once the researcher has used Idea Coach to create questions, such as those shown in Table 1, the researcher select questions and/or adds in questions of one’s own. These questions are automatically inserted into the template. The researcher is then prompted to provide four answers to each question. The Idea Coach can provide answers to each question, providing 15 answers for each question for each ‘run’ of the Idea Coach. The researcher need only select four answers, or fewer answers, providing other answers as desired. The answers can be edited, as they were in this study, generally edited for simplicity, and recognizing the nature of the project. In this study the answers were edited by introducing the sub-topic, followed by a colon, and then followed by the text. In this way the element would end up ‘making sense’ in the format of Mind Genomics, wherein one element was presented atop another, not connected with each other, but part of the vignette.

Table 2 presents the four questions selected from the 30 questions at the start of the study. These questions may or may not have appeared in Table 1 for the simple reason that each iteration of Idea Coach produces different sets of questions. Table 2 also presents the four answers to each question.
 

Table 2: The four questions and the four answers to each question

tab 2
 

Once the questions and answers are selected, the Mind Genomics program creates combinations of elements. These combinations are called vignettes. A vignette comprises a minimum of two elements, and a maximum of four elements. The structure of the vignette is specified by a layout called an experimental design. The experimental design for Mind Genomics was created to ensure the following properties:

  1. Every respondent evaluates exactly 24 vignettes
  2. The 24 vignettes are set up so that each of the 16 elements appears exactly five times and is absent 19 times.
  3. A vignette has either two, or three or four elements.
  4. A vignette can have at most one element from a question, often has no elements from a question, and never has more than one element from a question. This property is important. It is a bookkeeping feature which ensures that a single vignette can never have two different pieces of information of the ‘same type’. The problem becomes obvious when instead of the elements selected here, the elements are prices and brands. In the latter case we want to ensure that a single vignette can have at most one price, not two prices, which would be self-contradictory.
  5. The underlying experimental design ensures that the 16 elements are statistically independent of each other. This statistical independence and the specific nature of the design allow the researcher to use statistical modeling (OLS, ordinary least-squares regression) to relate the presence/absence of the 16 elements to the dependent variable. Usually the dependent variable is the rating assigned, a simple transform of the assigned rating, or response time (the time elapsed between the presentation of the vignette to the respondent and the respondent’s rating, captured by the program).
  6. A special feature of the experimental design is that it is permuted [10,11]. The permutation means that the underlying mathematical structure is maintained, but each respondent evaluates different combinations. From the practical point of view, the permutation means that the research ends up covering a lot more of the underlying ‘design space’. Rather than having to have a good idea of the ‘right test stimuli’, viz., the right test combinations, and then test those ‘right combinations’, the permuted design tests many more combinations. The patterns in the data emerge more powerfully when the large design space is explored, rather than when one focuses on what ‘thinks’ to be a promising area.

Study Execution on the Internet

The actual study is run in a straightforward fashion, using the Mind Genomics program, www.BimiLeap.com. The program both guides the set-up of the study, as well as executes the study. Once the researcher has set up the study, the next step is to ‘order’ respondents. Traditionally, this process has been arduous, typically because over the past decades respondent participation has dropped. The simple reasons are that people are busy, but also one can scarcely do anything in the commercial world without a sweetly phrased request inquires whether the person would be willing to complete a short survey about the experience. One or two such requests are acceptable, but one can expect resistance, despite the motto of the Marketing Research Association that ‘Your Opinion Counts.

To remedy the issue of difficulty in recruiting respondents, the BimiLeap program contains a built-in facility to both issue invitations (links to the study), or to pay for respondents, specifying these respondents by a set of screens showing qualifications. The paid request is immediately sent to the associated ‘on-line panel provider’, Luc.id, which aggregates respondents, and provides the necessary respondents in a short period of time, perhaps an hour or so for 100 qualified respondents. This speed means that the entire study can be set up in 30 minutes or less, and executed in 90 minutes or less for 100 easy-to-find respondents. The result is a study executed form start to finish within the space of two or two and a half hours.

The actual study begins with a short introduction about the project, a set of classificaiton questions (age and gender fixed, and then a no/yes question of having been bullied in school). The respondent then proceeds to the actual evaluation of the 24 vignettes. The orientation is short, providing almost no substantive information. The objective is for the orientation to simply introduce the topic. The actual information is presented in the vignettes, the combinations of the elements. Table 3 below presents the orientation sentence, the rating question, and the five answers comprising a Likert scale.

Table 3: The orientation sentence, the rating question and the five point rating scale

tab 3

Initial Analyses – Frequency of Selected Ratings by Groups of Respondents

Mind Genomics studies generate a great deal of data. We can approach the analyses in at least two different ways. The first way is to look for different patterns of responses, recognizing that we are dealing with different vignettes across all 110 respondents or 2680 vignettes, without the test stimuli having any cognitive meaning at all, other than being test stimuli, the responses to which are being measured.

Following the foregoing analysis, we can examine the frequency of ratings by groups. The first set of groups include the Total Panel, the genders, the ages, the groups who say they were bullied before versus not bullied before. The second set of groups is the mind-sets, individuals who think similarly to each other. We look at the 110 respondents divided into two mutually exclusive mind-sets and then three mutually exclusive mind-sets. Finally we look at the position of the vignette during the sequence of 24 vignettes (first to fourth quarter), and at the vignettes rated quickly (< 3 seconds) versus at the vignettes rated slowly (>= 3 seconds).

Table 4 shows the frequencies. There are occasional differences of 8% or more, especially for the ratings of ‘makes sense’ (5 and 4). There are a few differences for ‘makes no sense’ (1 and 2), and very few for ‘don’t know’. We conclude from Table 4 that there are differences in the measure ‘makes sense’, but beyond that deliberate overlooking of the cognitive meaning hampers our knowledge. It will be with the knowledge of what the elements ‘mean’ that we will make progress.

Table 4: Frequency of ratings assigned by the different, identifiable groups of respondents or vignettes. The numbers in the first three columns of each row should add to 100%.

tab 4

How Elements Drive Ratings of ‘Makes Sense’ and ‘Doesn’t Make Sense’

The previous section considering the frequency of ratings showed that simply measuring responses to test stimuli suggests some differences among groups, although one would be hard pressed to learn more about the process of decision making. Fortunately, the cognitive richness of the test stimuli, viz., the simple statement, can be used by the researcher to reveal what the respondent may ‘think’ about a topic, even when the respondent herself or himself cannot articulate the rules by which the respondent makes a simple decision. Indeed, quite often exit ‘comments’ by respondents participating in a Mind Genomics study end up with the respondent insisting that it was impossible to ‘find the right answer that was deemed appropriate’ and that much of the answering felt like ‘guessing’ rather than like a reasoned rating.

A deeper analysis of the ratings shows, time after time, that there is a clear set of rules that can be inferred by the pattern of ratings, but only when the ratings themselves can be related to the presence/absence of the specific messages in the vignettes. That is, the pattern of ratings themselves fail to provide information as Table 4 above suggests. Yet, as the remaining tables in this paper will reveal, there are clear, consistent, and interpretable patterns, especially when we bring to bear the combination of OLS (ordinary least squares) regression, coupled with cluster analysis to identify similar behaving groups in the population.

OLS regression, colloquially known as ‘curve fitting’, relates a dependent variable to one or more independent variables. For our study, the regression equation is written as:

Dependent variable = k0 +k1(A1) + k2(A2) … k16(D4)

In words, the expected value of the dependent variable (e.g., Makes Sense, Top2, Rating 54) is the sum of 17 numbers. The first number, k0, is the additive constant. It is the expected the top of the dependent variable (e.g., Top2, Makes Sense) in the absence of elements. Of course, all 24 vignettes evaluated by each respondent comprised a minimum of two elements and a maximum of four elements, so the case of a vignette without elements is hypothetical. Yet, the additive constant (also called the intercept by statisticians) is estimated, and is considered to be a correction factor. We can use it as a baseline, from which all elements either add or subtract. The additive constant is usually high when the dependent variable is ‘Top2, Makes Sense’, and usually low when the dependent variable is ‘Bot2, Does not make sense’.

The regression analysis returns with positive coefficients and with negative coefficients. For our analysis we will present only the positive coefficients of magnitude +2 or higher. The blank spaces in the tables of coefficients actually have numbers, negative or zero coefficients, but in the interest of discerning the pattern we are primarily interested in the positive coefficients. In actuality, presenting the positive and negative coefficients clouds the results.

The top portion of Table 5 presents the positive coefficients for the Top2, viz., for the Total panel, two genders, three ages, and the first six versus the last six vignettes in the set of 24 vignettes. The additive constants are all high, but the highest for the younger respondents, ages 15-18 (additive constant 83) and ages 19-21 (additive constant 78). This is noteworthy. It says something about the proclivity of younger respondents to agree with the statements about bullying, especially since respondents ages 22-26 show a much lower value (additive constant 61). It is important to recognize that this type of granular information could not have emerged from conventional research, and may not have been recognized without the use of OLS regression.

Table 5: Parameters of the models for ‘Make Sense’ (Top 2), and for ‘Does Not Make Sense’ for self-defined groups, and for first and last test orders.

tab 5

Moving now to the granular level of elements, we see many empty cells. These cells belong to elements which failed to drive a perception of agreement beyond the general proclivity to agree evidenced by the additive constant. What strikes us as noteworthy is that the majority of strong performing elements occur in the columns devoted to order of testing. There seem to be two types of elements, those which desensitize with exposure, and those which sensitize with exposure. The magnitude of the effect can be dramatic, especially when we see that the additive constants are virtually equal (63 for vignettes 1-6; 61 for vignettes 19-24).

Sensitizing elements (stronger tor vignettes 19-24, weaker for vignettes 1-6)

To stop bullying: Encourage students to speak up if they witness bullying.

To stop bullying: Bullying should be discussed openly and regularly, both in class and in assemblies.

Desensitizing elements (stronger for vignettes 1-6, weaker for vignettes 19-24)

Likely bully: People who are unhappy with themselves

Likely bully: People who are insecure

Likely bully: People who have mental health issues

When the analysis is reversed, focusing now on what does not make sense (BOT2), Table 5 suggests a different picture. As expected, the additive constants are quite low, hovering around 20. In turn, a low additive constant ends up allowing a variety of elements to emerge. Table 5 shows the greater number of low coefficients. Worthy of note are the three element responded to strongly by the youngest group of respondents, ages 15-18. Here are the elements which do not make sense to them:

Reason others bully: They want to make someone else feel bad

Reason others bully: They’re mean

Victims feel: Powerless

Table 5 gives us somewhat of a sense of the mind of the respondent. We find some messages to be strong, most messages to be weak. One hypothesis which emerges is that people may differ from each other in part of their life histories. For our study of bullying, a question which comes to mind is whether having been bullied (or at least answering YES on the up-front classification questionnaire) reveals itself in the pattern of responses to the elements.

Table 6 shows the parameters of the equations developed from the 38 respondents who said that they were bullied versus the parameters of the equation developed from the 72 respondents who said they were not bullied.

Table 6: Parameters of the models for ‘Make Sense’ (Top 2), and for ‘Does Not Make Sense’ for those who reported that were bullied versus not bullied.

tab 6

There are some differences, not in the additive constant (baseline), but in the elements. Those who said that they were bullied find more elements to ‘make sense’ than those who said that they were not bullied. In terms of not making sense, those who were not bullied find more elements not to make sense.

Mind-Sets

A foundational principle of Mind Genomics is that for the world of the everyday there are differences in the way that people make decisions. These differences emerge in the granular level of the everyday, not necessarily in a way which sets one person apart from others. Researchers might call these individual differences, often recognizing that they could end up being a vexatious source of variability, hindering the signal, but signifying nothing. In contrast, Mind Genomics processes this variability through clustering respondents on the patterns of their coefficients for their models. The clustering uses all 16 coefficients for the respondent, viz, positive coefficient, 0’s, and negative coefficients, the latter two coefficients not shown in the tables. The method k-means clustering, uses as a distance measure the value (1-Pearson correlation between the corresponding sets of 16 coefficients for two people [12]).

The k-means program extracted two mind-set and three mind-sets, using the coefficients relating the presence absence of the elements to the ratings of ‘make sense’ (viz., Top3) The 110 sets of coefficients were used for in the k-means, with the additive constant not included Table 6 presents the non-zero coefficients, first for TOP2 (Makes sense), and for BOT2 (makes no sense).

The two-mind set solution suggest one group one group focusing on WHO is a likely bully, and a second group focusing on HOW TO STOP a bully. The three mind-set solution distinguishes among WHO is a likely bully from How to STOP a likely bully, and less clearly but still emerging from the reason WHY person is a bully.

It is remarkable that in the face of such a complex task, respondents are able to focus on what is important to them. It is even more remarkable when we see that only with clustering the responses do these groups emerge clearly, in a way that could not possibly be biased (Table 7) [13].

Table 7: Parameters of the models for ‘Make Sense’ (Top 2), and for ‘Does Not Make Sense’ for two and three mind-sets extracted from the coefficients using k-means clustering.

tab 7

Discussion and Conclusions

The extensive literature on the topic of bullying has been created by adults, for adults, using the behavior of children towards each other. The inevitable effect of the research effort and the published result is to describe and explain the behavior of children as a combination of anthropology, sociology, and psychology. The researcher sits on the outside, observing the behavior, or talking to those involved in the behavior. Afterwards, the researcher translate the personal experience of the children into ‘adult, science-speak’, replete with statistics when relevant. Of course research ethics do not permit the researcher to induce bullying as part of an experiment, requiring observation and storytelling, rather than experimentation.

It is obvious that young researchers can construct simple questionnaires, and by administering these questions to the correct people the young researcher can learn a lot about the world from the point of view of other people. One need only look at the exercise of interviewing someone older about one or another life experience, the type of research that schools use to introduce students to the research process. Within that framework, the Mind Genomics study reported here presents the way people think about bullying. The data could be reported in that fashion.

The important activities of the Mind Genomics efforts occur at the start, when the study is designed and the questions/answer are developed, and at the end, when the results are analyzed. The Mind Genomics approach, modified by adding a coaching feature, enables the younger researcher to investigate topics in great depth, doing work which by virtue of the coaching and templating becomes valuable. When looking at the execution of the study, and the results, it would be hard to believe that the author of the study is a grade school student. That is precisely the point. The student has now done professional level research. The topics might be thought about initially by the mind of a grade school student, but quickly the execution and the results bring the student to a higher degree of understanding of the topic. It is the mind of the grade school student which frames the topic and selects the question. It is the research approach, the AI coaching, and finally the easy to read results emerging from powerful but ‘hidden’ analyses, which end up helping the student to get excited at the prospect of discovery, and accelerate the process of self-education.

References

  1. Bradshaw CP (2015) Translating research to practice in bullying prevention. American Psychologist 70: 322-332. [crossref]
  2. Dake JA, Price JH, Telljohann SK, Funk JB (2003) Teacher perceptions and practices regarding school bullying prevention. Journal of School Health 73: 347-355. [crossref]
  3. Evans CB, Fraser MW, Cotter KL (2014) The effectiveness of school-based bullying prevention programs: A systematic review. Aggression and Violent Behavior 19: 532-544. [crossref]
  4. Craig W, Boniel-Nissim M, King N, Walsh SD, Boer, M, et al. (2020) Social media use and cyber-bullying: A cross-national analysis of young people in 42 countries. Journal of Adolescent Health 66: S100-S108. [crossref]
  5. Sedgwick R, Epstein S, Dutta R, Ougrin D (2019) Social media, internet use and suicide attempts in adolescents. Current Opinion in Psychiatry 32: 534. [crossref]
  6. Moskowitz HR, Gofman A, Beckley J, Ashman H (2006) Founding a new science: Mind Genomics. Journal of Sensory Studies 21: 266-307.
  7. Porretta S, Gere A, Radványi D, Moskowitz H (2019) Mind Genomics (Conjoint Analysis): The new concept research in the analysis of consumer behaviour and choice. Trends in Food Science & Technology 84: 29-33.
  8. Mendoza, CL, Mendoza CI, Rappaport S, Deitel J, Moskowitz HR (2023) Empowering Young Researchers: Understanding the mind of prospective aides regarding elderly clients.
  9. Mendoza CI, Mendoza CL, Rappaport S, Deitel J, Moskowitz HR (2023) Empowering Young Researchers: Exploring and understanding responses to the jobs of home aide for a young child.
  10. Mendoza CL, Mendoza CI, Rappaport S, Deitel J, Moskowitz HR (2023) Empowering young researchers to think critically: Exploring reactions to the ‘inspirational charge to the newly-minted physician.
  11. Gofman A, Moskowitz H (2010) Isomorphic permuted experimental designs and their application in conjoint analysis. Journal of Sensory Studies 25: 127-145.
  12. Likas A, Vlassis N, Verbeek JJ (2003) The global k-means clustering algorithm. Pattern Recognition 36: 451-461.
  13. Hardy MA (1993) Regression with Dummy Variables. Sage.
fig 1

Communication Preferences of Obese Female Adolescents with Clinicians

DOI: 10.31038/AWHC.2022534

Abstract

This study tests the use of specific brief narrative messages that clinicians may use with obese female adolescents regarding body images. The sample comprised 102 obese adolescent females. Each respondent evaluated a unique set of 48 combinations (vignettes) of messages, created from a base size of 36 messages, each set vignettes evaluated by a respondent specified by an underlying experimental design. Regression analysis at the individual respondent level generated coefficients, which were clustered to real three interpretable groups of respondents (viz., three mindsets). These mindsets, limited to the granular topic of body image are: Known need of control (33%), Self-condemnation and shame (46%), Feeling ugly, panicky, and victimized (24%). A subsequent application created a predictive algorithm, a personal viewpoint identifier which is a six-question tool, based on mathematical clustering. The pattern of ratings assigned a new respondent to one of the three mindsets. Clinicians may use effective communication messages by mindset-belonging to influence body image and prevent eating problems in female adolescents.

Introduction

The term body image (BI, hereafter) describes one’s perceptions about one’s own body that develops throughout adolescence [1,2]. Body image entails a perceptual dimension referring to an individual’s self-perception of their appearance and an attitudinal dimension referring to four components: affective, cognitive, behavioural, and satisfaction. The affective component refers to the comprehension of feelings relating to one’s appearance. The cognitive component refers to knowledge about body image. The behavioural component considers body-checking behaviours and actions to avoid situations or objects that evoke body image concerns. The satisfaction component concerns a person’s appreciation over their body as a whole or to specific parts [3].

Female adolescents, more than males, associate higher and increasing Body Mass Index (BMI) with lower self-esteem, routinely evaluate their body, perceive their social worthiness as determined by their physical attractiveness, and tend to obsessively worry about their physical appearance [9,10]. DBI has been associated with low self-esteem, depression, anxiety, poor social functioning, poor health-related quality of life, and concerning unhealthy eating behaviours (i.e., fasting, vomiting, or laxative abuse) leading to malnutrition, noncommunicable diseases, obesity, diabetes, cardiovascular disease, cerebrovascular incidents and even cancer [4].

Female adolescents stated that DBI is a “touchy subject” [5]. Although clinicians are aware of the importance of communication to promote a positive BI in female adolescents, and 74% of clinicians in a study reported discussing DBI with adolescent patients, 85% of female adolescents reported that they wanted to talk about their DBI with their clinician, but never held such a conversation [2]. Clinicians acknowledge that they feel uncertainty and have no confidence to communicate with female adolescents on DBI [5,6]. Thus, while communication may mitigate the risk factors of DBI, research on how to discuss the topic in practice is scant [2,5]. This study begins to close the gap in the state-of-the-art, exploring preferences of female adolescents regarding clinician-adolescent communication on DBI [5,7,8]. This study seeks to identify and crystalize specific communication messages to support clinicians’ choices of the right messages in communication with adolescents on DBI.

Test Stimuli – Questions, Answers (Elements) and Vignettes

Mind Genomics works by presenting combinations of elements to the respondent, obtaining a rating of the combination, and then deconstructing the rating of the combination to the part-worth contribution of each element. The experimental design uncovers the preference for communication while inhibiting the social desirability bias of respondents as often occurs in surveys. The experimental design approach design has been used to understand preferences in different health contexts [9-11].

We begin with the raw materials, the elements. Mind Genomics comprises different structures, allowing a flexibility in the research process. The most popular version is the so-called 4×4 design, with four questions, and four answers (elements) for each question. A second popular version is a 6×6 design, entailing six questions, and six answers to each question. This second version, 6×6, was chosen for this study. Table 1 shows the six questions and the 36 elements.

  1. The independent variables are categories of communication based on previously used scales assessing perceptions regarding DBI [12-14].
  2. Perceived weight status was assessed with the question: “At this time do you feel that you are..”
  3. DBI was assessed with a modified version of the Body Shape Satisfaction Scale [15].
  4. Unhealthy and extreme weight control behaviours included: fasted, ate very little food, used a food substitute (powder or a special drink), and skipped meals [16].
  5. Binge eating was assessed with questions such as: “You eat so much food that you would feel embarrassed if others saw you” [17].
  6. Self-weighing was assessed by asking adolescents to indicate how strongly they agreed with the statement, “I weigh myself often.”

Table 1: The six questions (categories), and the six answers to each question

tab 1(1)

tab 1(2)

Mind Genomic then combines the elements in vignettes, which for the 6×6 design comprises 48 vignettes three or four elements, respectively. The combinations are not done randomly, but rather constructed according to an underlying experimental design. The design specifies the 48 combinations, ensuring that each element appears equally often, ensures that the 36 elements are statistically independent of each other, and that each respondent evaluates a unique, different set of combinations. This is called a permute experimental design [18]. The permutation allows the researcher to investigate a great of the underlying ‘design space’ of different combinations. Rather than having the researcher somewhat ‘know the promising combination’, the permuted design allows true exploration, even in the total absence of any knowledge. Mind Genomics thus differs from conventional research, sacrificing precision of measurement through replicated measurements of a few test vignettes to precision of understanding of the topic through exploration of much more of the design space. To give an example here, the Mind Genomics effort explored 4896 combinations in the design space rather than exploring 48 vignettes in the design space.

The underlying experimental design serves another purpose as well, specifically Bookkeeping. The experiment design is created so that mutually contradictory elements, viz., elements of the same type but conveying different messages, end up in the same category. The underlying experimental design ensures that a vignette has at most one element or answer from a viz., question. The happy outcome is that the vignettes never present directly contradictory elements to a respondent, at least when one considers a simple reading of the vignette. The elements themselves were relevant to the world of the clinician regarding DBI [19].

Executing the Study on the Internet

Respondents began with an orientation page, signed an informed consent for participation and publication, completed three demographic questions for classification, and finally rated the specific set of 48 combinations of messages corresponding to their own individual experimental design.

The 36 messages were presented in 48 combinations. Every respondent evaluated a unique, different set of 48 combinations [20]. The experimental design varies messages to create different combinations of messages, each combination comprising a minimum of three and a maximum of four messages. The experimental design is set up so that mutually contradictory messages cannot appear together in the same combination. The outcome variable was preferences of adolescent females regarding communication with clinicians on DBI.

It is important to note that the experimental design ensures that the elements will be statistically independent of each other, and the array of 36 elements will allow OLS (ordinary least-squares) regression to estimate the coefficients of the model created for each respondent separately. The rating question was “how important are these vignettes in communication with your clinician on BI?” Each respondent rated the 48 unique combinations using an anchored 9-point scale (1 = “I prefer not to talk about this in communication about my DBI”; 9 = “I would like to talk about this in communication about my DBI”).

After rating the 48 vignettes, the respondent completed a short socio-demographic questionnaire to define aspects of the respondent, without revealing any other personal information. The self-classification question recorded ethnicity and socio-economic status (e.g., higher education level of either parent; family eligibility for public assistance; eligibility for free or reduced-cost school meals, and parental employment status) [16,21].

Data Analysis

The 48 combinations created for each respondent, comprise a stand-alone experimental design for that respondent. Each of the 36 messages is statistically independent of the other 35 messages. The experimental design allows the analysis of the results using OLS (ordinary least-squares) regression, at either the individual respondent level (within-subjects analysis), or the analysis of groups of respondents (OLS) [22].

During the development of Mind Genomics from the 1990’s onwards, it has become a standard practice to convert the Likert Scale to a binary scale, to make the analysis and the interpretations more intuitive. Researchers and especially those who use the data for decisions encounter problems interpreting averages, often asking about the meaning of averages in everyday terms. To simplify the process the Mind Genomics process first transforms the ratings, to move the 9-point scale to a binary, 0/100 scale. By convention, ratings of 1-6 are transformed to 0, ratings of 7-9 are transformed to 100, and then for each newly transformed rating a vanishingly small random number (<10-5) is added.

The foregoing procedure now generates 102 sets of 48 rows each. Each row corresponds to one respondent, and one of the 48 vignettes evaluated by the respondent. The database is set up for OLS regression. The first set of columns record the respondent identification, the order of the vignette (1-48), and one column for each of the information questions asked in the self-profiling classification. The second set of 36 columns are reserved to code the presence of an element in a vignette (coded by the number ‘1’ for the column corresponding to an element), or the absence of an element in a vignette (coded by the number ‘0’). The final set of columns show the rating assigned by the respondent (viz, 1 to 9), and the transformed value of that rating (viz., 0 or 100).

For the OLS analysis, the independent variables are the 36 messages, coded 0 or 1 (absence/presence). The OLS model was formulated as Transformed Binary Rating = k0 + k1 (message A1) + k2 (message A2) … + k36 (message F6). For descriptive purposes, we will look at each of the 37 numbers as a measure of ‘describes me’. The Mind Genomics program computes the additive constant and all 36 coefficients, returning a great deal of data. To uncover patterns, we will present only the positive coefficient > 1. The smaller coefficients will not be shown, even though they were computed. The appropriate cell in the table will be left blank. Furthermore, for strong performing elements, those with coefficients of 8 or higher, the cell will be shaded to drawn attention to these elements.

The additive constant of the OLS model is a baseline, an estimated parameter, providing a measure of how likely it is for an adolescent to say ‘this describes the way I would like to talk to my clinician’, albeit in the absence of messages. Of course, the underlying experiment design ensures that every vignette comprises 3-4 elements, so the additive constant, is a baseline, a strictly estimated parameter.

The individual coefficients show the driving power of the messages. Continuing with the example but moving to the coefficient, a positive number of +8, means that when the message is incorporated into the combination, an additional 8% of the respondents are likely, on average, to say that they ‘this describes the way I would like to talk to my clinician.

People differ in their attitudes and perceptions, as well as their needs and wants. The self-profiling classification allows the creation of equations either for the Total Panel, or for any specified group. The OLS regression simply calculates the additive constant and the 36 coefficients based upon all the data appropriate to define that subgroup.

Finally, person-to-person variation may not necessarily depend on who the person is or what a person ‘believes’ for a specific situation. One’s perceptions and values may not clearly co-vary with who a person IS, or how a person THINKS about a general problem. There may be groups of people who are similar, not necessarily for all of the topics of their lives, but perhaps only for the granular topic being investigated. These are mindsets, groups of people who are similar in a specific topic area, based upon the similar of the pattern of their coefficients, in this case their 36 coefficients. To discover these groups, so-called ‘mindsets’, requires the use of a simple clustering method (viz., k-means). The clustering applied to the patterns of coefficient reveals new-to-the-world groups of individuals showing similar patterns of preferences in communications, a way to understand the different needs of the groups. [23].

Sample

Respondents were 102 obese female adolescents from the greater New York area, ages 13-19 years old. Respondents gave their informed consent for participation and publication. The size of the sample is consistent with the suggested sample size in conjoint analysis studies, particularly when aiming at stability of coefficients rather than stability of means [24]. Since DBI entails both a physiological indicator (BMI) and a subjective construct of BI, inclusion criteria for the study were a BMI of 30 and above and respondents’ self-definition of themselves as being overweight.

Relating the Presence/Absence of the Elements to the Binary Transformed Rating

The heart of the Mind Genomics analysis is the set of coefficients emerging from the regression analysis. Recall that the OLS regression deconstructs the response to the vignettes into the part-worth contribution of the 36 elements. Respondents cannot ‘game’ the system and give the correct analysis, simply because in the rapid process of stimulus/response, the typical evaluation time is about 3-4 seconds. There is simply no time for the respondent to try to ‘guess’ the right answer. It is simply impossible to do. Respondents report that they simply look at the vignette, and assign a value, often stating that they feel that they are guessing.

Table 2 presents the regression coefficients for the total panel and for the three mindsets as created by k-means clustering analysis. Messages are sorted by the strong performing coefficients for the three mindsets. The same message may appear twice when it is a strong performer. The total panel shows only two strong performing messages. These are “You eat until you are full and don’t pay attention to portion sizes”; and “You have erratic eating habits” reflecting erratic eating habits. It is when the data from the three mindsets are laid out that the patterns emerge.

Table 2: Coefficients for the messages by Total Panel and by the three emergent only positive coefficients greater than +1 are shown to allow patterns to emerge. The table shows strong performers for each mindset. (MS).

tab 2(1)

tab 2(2)

Table 2 suggests that a seemingly flat pattern of coefficients from the total panel may result from the combination of groups of adolescent females with different, often opposite, points of view. Groups of adolescents with similar response patterns emerge from the patterns in the data, patterns which are interpretable and parsimonious. What members of one mindset prefer to discuss in communication with clinicians on DBI may be irrelevant to adolescents in other mindsets. Furthermore, the coefficients for the mindsets are much higher, suggesting that the results from the total panel hide the underlying narratives by averaging dramatically different subgroups with different ways of thinking about their preferences. K-means clustering shows that there are distinct groups with different points of view regarding communication on DBI.

The Distribution of Mindsets across the Population

Table 3 shows that the three mindsets are distributed similarly across demographic attributes of the female adolescents, whether those are geo-demographics, parent’s education, or actual weight. There are some departures from random distribution, but there is no clear pattern and no explanations for the departures. Most of the adolescents are either Caucasian or African American. Mindset 2 seems to include more respondents who live in the suburbs. Mindset 3 comprises more African Americans and is over-represented by those with large families. Its members feel ugly, panicky, and a victim.

Table 3: Distribution of respondents into different groups, for Total Panel and for the three emergent min-sets

tab 3(1)

tab 3(2)

tab 3(3)

Assigning an Adolescent to a Mindset

This study reveals the existence of mindsets in the population of adolescent females and provides an organizing principle for clinicians by which to choose messages on DBI in communication with adolescents. For the data to become ‘actionable,’ it is necessary to develop an easy-to-use a predictive algorithm to rapidly assign an adolescent in the clinic to one of the three sample mindsets. Using a Monte-Carlo simulation process, we created a Personal Viewpoint Identifier (PVI) based on the mindsets data (Table 2). The PVI identifies a set of six original messages which can be scored on a two-point scale. Each of the 64 patterns of responses to messages assigns an adolescent to one of the three mindsets. The PVI presents the algorithm showing the six distinguishing messages taken from the data in Table 2. The pattern of response to those six messages assigns a female adolescent to a specific mindset, and may be linked to a video, to a website, or simply to the clinician.

Figure 1 presents the PVI which can be found at: https://www.pvi360.com/TypingToolPage.aspx?projectid=1266&userid=2008. The left panel shows the PVI instrument, and the completed answers from one person. The right panel shows the three mindsets. The shaded text presents the mindset to which the person is assigned. The tool is designed to be used in clinical work, as well as on the internet.

fig 1

Figure 1: The PVI which can be found

Discussion and Conclusions

This experimental design explored preferences of adolescent females regarding communication messages with clinicians on their DBI. The current study appears to be the first one to investigate preferences of adolescents regarding communication with clinicians on DBI. Within that framework, the study reveals the potential of tailoring, possibly enhancing the communication based upon the uncovered preference patterns of mindsets. The study revealed three mindsets, a finding which provides deeper insight to the minds of the adolescent.

Adolescents belonging to Mindset 1 (31%) seem to have an internal locus of control and prefer communication messages which accord with, and which encourage their internal locus of control [25]. They prefer to feel that they, not society nor their parents, have the control to change their thinking and behaviours, with the clinician by their side. They prefer communication which focuses on their responsibility and choices regarding DBI. For example, a potentially acceptable phrase might be: “You are independent and don’t get along with your family because you think they are too controlling”. Effective communication messages for members of Mindset 1 should focus on providing them with a higher sense of control through higher awareness of their feelings about their weight and behaviours due to their DBI, messages focusing on the possible reduction of their health-related quality of life [26].

Adolescents belonging to Mindset 2, comprising almost half of the population (45%), are female adolescents with low self-esteem and a negative BI increasing the risk for eating disorders [15]. Members of Mindset 2 prefer communication which encourages their reflective thinking:” Do you think that if you were thinner, you would be happier”; “Do you think that if you were thinner, you would be popular?” This finding confirms previous findings regarding adolescents’ expectations that the communication with clinicians will tap into mental and emotional aspects of DBI [34]. It also supports suggestions to convey genuine caring, active listening, and compassion to facilitate communication on DBI [27,28].

Adolescents belonging to Mindset 3, the smallest group (24%), appear to have a strong external locus of control. They feel they are victims of circumstances that are beyond their control [25]. They internalize the opinion of others, and in turn judge themselves [29]. This finding is in line with a study that contended that obesity should be communicated as driven by a psychological cause rather than a behavioural cause to mitigate prejudice and stigma [30]. Mindset 3 responds positively to the following communication messages: “You feel self-conscious about your appearance”; “You feel ugly in comparison to your friends”; “Your parent or caregiver does not cook healthy, balanced meals for you”; “When you go shopping, you buy clothes a size smaller than you really need, hoping that you will soon lose weight and they will fit you”; “You were born with big bones or a big frame”; “Your family has a history of health-related problems as the result of unhealthy eating lifestyles”. Communication with members of Mindset 3 should provide a sense of order, highlight dangers of unhealthy weight control behaviours, and enhance their internal locus of control [25,31]. Members of Mindset 3 respond when they sense that the communication with clinicians on DBI will be supportive, engaging, empathic, and authentic, indicating that the clinician cares about them as an individual [5].

This study has several contributions. Theoretically, the study extends the existent knowledge revealing preferences of obese female adolescents in sensitive communication with clinicians regarding their dissatisfaction with body image. The data suggest at least three mindsets of adolescents, showing the pattern of distinct preferences of adolescents in each mindset for specific communication messages of clinicians about DBI. Methodologically, the experimental design overcomes typical biases of survey questionnaires enabling to test many combinations of messages reflecting our complex reality [33].

In terms of practice contribution, this study developed a predictive algorithm enabling clinicians to quicky assign adolescent females in the clinic to a mindset in the sample, supporting clinicians in their communication on DBI with female adolescents in the clinic. Set-tailored communication messages may be helpful from a therapeutic standpoint to build trust of adolescents in the clinician, promoting adolescents’ perseverance throughout change processes [34]. Mindset-tailored communication may mitigate DBI among obese female adolescents, through trust in the clinician, perhaps preventing future disordered eating behaviours and extreme weight control behaviours [5]. Communication messages should correspond to the preferences of adolescents by mindset membership.

Currently, clinicians may discuss clinical issues with adolescent females (i.e., weight, growth, nutrition), but patient-centred communication on DBI requires clinicians to meet the communication preferences of the female adolescent and understand what troubles her. Currently, communication on DBI is sub-optimal as clinicians are not trained at patient-centred communication, facilitating trust, and open communication on DBI [5]. Female adolescents judge the communication with clinicians by the clinician’s communication skills, the extent of interest of the clinician in them as individuals, and the extent of sensitivity in discussing DBI [34,35]. Therefore, it may be helpful for if clinicians could customize the communication according to their mindset-membership. The creation of a predictive algorithm enables clinicians to better understand the adolescent and promote a positive BI through mindset-tailored communication messages. The ability of clinicians to tailor the communication to the mindset-belonging of an adolescent, almost at the start of the relationship, provides new opportunities for interactions which may improve trust in the communication on DBI and promote health, wellbeing, and life satisfaction. Last, our findings reaffirm the need to train clinicians to raise their awareness of differences among female adolescents on communication preferences and psychosocial issues associated with DBI [17]. Clinicians should set communication goals in mind (providing information, reducing distress, increasing adolescent satisfaction, and encouraging hope) while prioritizing efforts to reduce distress [36].

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Empowering Young Researchers to Think Critically: Exploring Reactions to the ‘Inspirational Charge to the Newly-Minted Physician’

DOI: 10.31038/PSYJ.2023521

Abstract

The study is part of a program set up to encourage young researchers (age < 18) to learn to think critically. The process shows the young researcher how to systematize aspects of daily life, create a database of specifics, and then explore the perception of these aspects by real people, using templated experimental designs and real ‘experiments’ on the web through a purpose-built application (www.BimiLeap.com). In this study respondents evaluated unique sets of 24 combinations (vignettes), each vignette presenting 2-4 statements which might have come from a ‘charge’ given to medical students and new doctors. Deconstruction of the response to these combinations at the level of individual respondents using regression analysis revealed which statements were perceived to be ‘important,’ which were perceived to be ‘memorable, which were both, etc. Cluster analysis of the individual coefficients from respondents revealed three mind-sets emerging, based upon the pattern of messages perceived to be important: MS1 – Dealing with the stress of the practice of medicine, MS2 – Dealing with difficult patients, MS3 – Aware of what is important to the patient.

Introduction

Empowering through Templated Experimental Design

This paper deals with the way a young researcher (Cledwin Mendoza) conceives of the way a medical school might inspire its students or give a ‘charge’ to a new doctor [1-4]. The topic of inspirational addresses given by senior professionals to newly-minted-professionals is of interest to middle school and high school students. It is their introduction to the world, a world about which they are curious, and excited to enter. The literature has publications on exhortations to the doctor, but virtually nothing from the vantage point of young people to whom the world of professionalism is both experienced but at the same time shrouded in mystery, the world of adulthood.

In this series of papers entitled ‘Empowering young researchers,’ we aim to look at the world of the everyday through the lens of the young researcher, in this case a junior high school student who is aware of the world, interested in the world, but whose sole experience is personal interactions with doctors, what he reads, and what he might see in the media. The research in this paper was designed and carried out using a new science, Mind Genomics. The objective of the research was to explore how ordinary people think medical students would react to inspirational and descriptive phrases regarding their teachings and ethical issues. The study is reported in the spirit of Mind Genomics, specifically a study of an everyday experience (the lecture or charge to a medical student), with the objective of learning what might be the inspiring messages and what might be the messages heard but soon forgotten.

Today’s computer technology allows people to do complex clerical tasks, often quite simply, by following a template. The template ensures that the person does the correct actions for each task, checks that the material is ready, and moves forward. As a consequence, the template ensures that the process will not run into a problem, at least in terms of the structure of the activity. Furthermore, what could be a complex, involved task might well become easy as the person filling out the template becomes conversant with the different steps. What could have been a boring, repetitive task may remain boring, but can be executed flawlessly over time. And, most of all, the learning to complete the template in general is fairly quick, although the material fitting into the template may itself be difficult.

Imagination about the Everyday

The history of science will show the increasing sophistication of the questions asked. From Aristotle who could only observe the world around and make conjectures, we move along to the world of experiments, where the notion was to understand how one variable ‘covaried’ with another variable, but more important, how one variable drove the other variable, viz., causation. As one readily recognizes today, the best experiments occur when the researcher is trying to understand how ‘nature’ works, searching doggedly in many cases for the rules of nature. At the same time there is the world of social science, the study of people, their social structures, motives, behaviors and so forth. Philosophers since Plato and Aristotle, and presumably many before them, were interested in why people behave the way they do. One never, however, feels that one can create a massive database about the many different aspects of people, at least in the social sciences.

Test Stimuli: Collaboration between the Researcher and AI (Idea Coach)

Mind Genomics works by creating a ‘bank’ of ideas (elements), presenting combinations of these elements to a respondent, having the respondent read and rate each combination on a fixed Likert scale, and then deconstructing the ratings to discover the contribution of each element. The process is mechanical once the elements are created. It is the creation of the elements which often stymies the researcher, leading to the abandonment of the project, or leading to an untenable delay in the project until ‘everything is just right’, everyone agrees with the choice of elements, etc. In the end, the task of creating the elements simply frightens many researchers, regardless of their experience or seniority.

The Mind Genomics template requires that the researcher select four questions which ‘tell a story’. For each question, the researcher is required to provide four answers. In the end, the raw material comprises the underlying story, knitting tighter the four specific questions, and the sixteen answers. Experience over three decades, since the early 1990’s, suggests that it is at this point in the process when many promising projects are simply abandoned by hopeful but frustrated researchers. The reasons for abandoning the project are not relevant, other than to say that there seems be a lowered tolerance for frustration in today’s researcher, and a belief that one should ‘know’ the answer, and select the ‘correct’ elements. Not being sure of ‘correct elements’ suffices to stop forward progress. Many researchers apparently suffer from “open-ended question anxiety.”

To remedy the problem, the Mind Genomics templated recently has been upgraded to incorporate Idea Coach, a user-friend tool based in OpenAI [5]. In the templated process, the researcher begins by providing the four questions. The researcher who would like ‘guidance’ chooses the Idea Coach button on the template form. The researcher then writes in a few sentences about the topic. Using that information, Idea Coach returns with up to 30 questions, from which the researcher can choose up to four. In the case where the questions do not all meet the researcher’s expectation, the researcher choose some, and repeats the Idea Coach effort, either with the same input (leading to a set of 30 questions, many of which are new), or with different input. The researcher ends up selecting four questions, some from AI, perhaps some from oneself, and can edit/polish before proceeding.

Table 1 shows the four questions. The Idea Coach was given the following background to the project:

Table 1: The four questions and the four answers (elements) for each question

tab 1

We created this study to see what important precautions young doctors need to face in the medical world. Also to help young doctors so they don’t make a serious mistake.

It is important to keep in mind that the formulation of the question comes from the mind of a young person, a middle-school student. The question is simple, direct, and not formulated in the typical manner that has become the pattern of today’s science. The question is one ‘about the world,’ in the naïve yet profound way that could only be asked by a young person. As the data will show, this type of questioning can lead to profound, powerful, and actionable results, as well as be a part of an easy-to-construct corpus of knowledge about the mind of people facing everyday life.

The quality of the questions shown in Table 1 come from the nature of the Open AI. The researcher’s task is made much easier. The task now morphs from trying to think about good questions to reading questions and selecting those which make sense for one’s project.

After the AI and researcher have collaborated to create/select the four questions, the BimiLeap templated system moves to acquiring four answers to each question. Once again the Idea Coach helps in this task, but requires the deeper involvement of the researcher. For the case of questions, Idea Coach required questions needing very little editing. Not so with the answers. Idea Coach returns with statements. These statements comprise the answers but the statements must be edited. For example, consider element A1. Idea Coach returned with the second part of the element, specifically ‘They need to have a passion for helping people and a strong interest in science and medicine.’ The BimiLeap program for Mind Genomics would be better when the sentence is given an ‘orientation’, such as the word ‘Required’. The authors changed the elements, inserting the orientation word(s), so that the elements were more meaningful. A good analogy to this is the placement of items in a menu under the proper headings (appetizer, main course, etc.,) rather than just having the food on the menu.

The second thing to note about the elements is that they are long, and well-crafted. Virtually all users of the BimiLeap program have commented on the fact that the questions, but more importantly the answers, move beyond what they might have generated had they been instructed to think about the elements, and do research to find elements. This up-front work, possibly taking hours and days, and often leading to frustration in the wake of progress seemingly out of one’s grasp, now takes approximately 20 to 30 minutes, with potentially far better results.

The final thing to note is that hitherto a long, arduous, and often frustrating effort to create questions literally from one’s mind is now replaced by a far more pleasant, intriguing learning experience. The researcher no longer has to feel alone in the effort to come up with questions and answers, but rather now engages in a focused experience of discovering and choosing. In some respects the creation of the elements evolves into its own learning experience, enjoyed by the researcher, with a motivating power to drive exploration of just-learned ideas. What was a difficult moment in the design of a Mind Genomics experiment now becomes perhaps the first learning experience. The Idea Coach, and AI, moves away from a simple aide to become a teacher who reveals new dimensions of a topic to a researcher deeply focused on the topic. Ideas that were not even in the ken of the researcher can now be explored in moments, as part of the research effort.

The History of Mind Genomics

Mind Genomics traces its history to three disciplines, psychophysics, statistics, and consumer research, respectively. Psychophysics is the oldest branch of experimental psychology, seeking to understand how to measure the internal perception of physical stimuli, such as the sweetness of sugar in a beverage. The origination of Mind Genomics can be traced to author Moskowitz’s studies as a graduate student in the Laboratory of Psychophysics in William James Hall, Harvard University, directed by the late Harvard professor, S.S. Stevens. During the latter part of Moskowitz’s tenure at Harvard, with a Ph.D. awarded for studies of taste mixture, it became obvious that the approach could be used to mix ideas, and obtain a measure of the mind in terms of responses to these ideas. The effort would have to wait until Moskowitz was safely ensconced as a scientist at the US Army Natick Laboratories, in Natick MA, where he could begin small scale studies of mixtures of ideas. The taste work would lead to the appreciation of human response mixtures as the relevant topic to explore, whether mixtures of ingredients, or mixtures of ideas [6,7].

Statistics, the second discipline, provides Mind Genomics with analytic tools. The history of science is often presented in terms of the researcher isolating all forms of extraneous noise, viz. unwanted variability, in order to study a phenomenon. We need only visit laboratories to see the apparatus used for these studies to get a sense of how proud the researcher is of the ability to study a phenomenon in ‘splendid isolation;, without the interfering noise. On the other hand, there are many effects where many variables interact with each other, and in the end produce a response. The traditional methods of isolating the variable and then studying the behavior of that variable simply cannot work. Rather, it is a matter of creating specific combinations, allowing the variables to interact, but at the same time allowing the researcher to measure the behavior of each variable, even though the variables are in a mixture. It is this discipline, statistics, specifically the area of experimental design, which constitutes the second foundation of Mind Genomics [8].

The third foundation of Mind Genomics is the world of consumer research, where the focus in on the complex, real-world stimulus, rather than on the artificially created world of the experimental psychologist. The consumer researcher focuses on what consumers are exposed to, how they react to situations which occur in everyday life. Consumer researchers are not attempting to understand the deep fundamentals of thinking and behaving, but rather interested in behavior in natural settings, dealing with responses to real test stimuli, or at least test stimuli which could be real.

The Test Stimuli

The foregoing history of Mind Genomics serves as an introduction to the nature of the test stimuli. The stimuli comprise combinations of elements, rather than single elements. The rationale is that only with combinations of elements can the test stimuli make sense, have ‘ecological validity.’ When we isolate the test elements, the 16 phrases shown in Table 1, instructing the respondent to rate each element, one at a time, we end up presenting the respondent with stimuli that are almost meaningless. Of course we are interested in the performance of each element, but it is very hard for the respondent to rate each element. It may be that the respondent and rate each element with the same mental rules, but more likely the respondent will end up shifting the mental criterion for the rating. That shift may be hard to imagine for these data, but easy to understand when we turn to elements which comprise brand name, price, nature of the product features, and location where the product is bought. When the rating sale is ‘interest in buying’, the respondent has a hard time using the same criterion. The data may look correct, but the reality is that during the course of evaluating the different types of elements the respondent may have shifted the criterion many time to be appropriate for the element.

To solve the foregoing problem, viz., of presenting ideas as simulating something real, Mind Genomics uses the power of experimental design to create combinations of elements. The experimental design for the 4×4 structure (four questions, four answers for each) comes up with 24 combinations. Within those 24 combinations, each of the 16 elements appears exactly five times, and is absent 19 times. A single vignette can have two, three, or four elements, but no more than one element from any question. The structure is set up so that the data from a single respondent can be analyzed by OLS (ordinary least squares regression), which as shown below, will reveal the individual level combination of every element to the response. Finally, the Mind Genomics design is set up so that each respondent sees different combinations. No respondents see the same combinations until the number of respondents exceeds 250. This property of different sets of 24 combinations created by the same mathematical scheme is called a permuted design structure.

The Orientation and Rating Scale

Mind Genomics studies are typically conducted with populations of respondents who are unfamiliar with the topic. They know that they will be reading and evaluating different combinations of messages, but have no idea that the combinations, also known as vignettes, will be created by experimental design. The respondent generally follows the instructions, doing what they are told. Thus, the simplest way to do the Mind Genomics experiment is to tell the respondent a little about the topic, viz., a sparse background, and then instruct the respondent to read the vignette and assign a rating using a defined rating scale.

Table 2 shows the instructions and the rating scale. The instructions are very simple. A principle of Mind Genomics is that it is more productive to provide a sparse orientation and let the individual elements in the vignette provide the necessary information about the topic. We follow this practice in our studies. Simplicity makes the task easy for a younger researcher because there needs to be very little deep knowledge behind the question.

Table 2: The orientation paragraph and the associated rating scale

tab 2

The rating scale in Table 2 merits more discussion. The rating scale actually encompasses two scales, one for important, and the other for remembering. Each point on the five point scale corresponds to one possible combination of thinking something is important and remembering the speech. Another thing to observe is that ‘remembering’ is first, and ‘important’ is second. In this way the scale is ‘broken up’ forcing the respondent to read the scale, rather than just remember a place on the scale, or at least that is the intention.

In order to prepare the data for analysis, it was first necessary to transform the scale. Users of data find it very hard to look at either the mean on the scale (which is meaningless for our broken up scale), or the distribution of ratings on the five-point scale. A simpler way to do things is to transform the scale values to binary (0 or 100). For simplicity, we abbreviate the word “Rating,” with “R” and the rating number. For example, “Rating 1” is abbreviated “R1,” etc. This study features two sets of transformations, one dealing with importance, and the other dealing with memorability.

1a. Important and Remembered (R5 only transformed to 100, rest of ratings transformed to 0)

1b. Not Important (viz., Not Necessary) and Not Remembered (R1 only transformed to 100, rest of ratings transformed to 0)

2a. Important (R5, R4 only transformed to 100, rest of ratings transformed to 0)

2b. Remembered (R5, R2 only transformed to 100, rest of ratings transformed to 0)

2c. Not Important (viz., Not Necessary) (R1, R2 only transformed to 100, rest of ratings transformed to 0)

2d. Not Remembered (R1, R4 only transformed to 100, rest of ratings transformed to 0)

With this transformation it becomes easier to discover patterns. The combination of rating scale points into those denoting ‘important’ vs. ‘not important’ allow us to isolate specific elements driving the perception of importance. Similarly, the combination of rating scale points into those denoting ‘remembered’ vs. ‘not remembered’ allow us the same power to isolate specific elements that the respondents feel will be ‘remembered’ by the medical students or young doctors. Keep in mind, however, that this experiment is done among the population of young people, ages 19-40. The same experiment could be done easily among medical students to discover whether they feel the same way.

Analysis 1

Do Different, Identifiable Groups of Respondent Score the Vignettes the Same Way?

In the foregoing introduction to Mind Genomics we made the point that each respondent in the study evaluated a unique set of 24 vignettes. This means that when we look at the distribution of ratings, we must keep in mind that we are not looking at the different groups of people evaluating the same stimuli, but rather evaluating different stimuli of the same type.

At this point it is worth pointing out that stopping here, just looking at the pattern of responses without any deeper analysis, would not be wrong. Indeed, the researcher who stops here, and supports her or his conclusions of similar patterns with conventional statistics, e.g., tests of statistical difference, would be lauded for defending the conclusion of ‘similar patterns’ using well-accepted statistics. Yet, as we will see below, when we move to the development of ‘mind-sets’, the researcher will be afforded the far deeper opportunity to understand the topic, one provided by the up-front efforts to create the test vignettes using experimental design.

Table 3 shows the average ratings for the six newly created dependent variables, for the key subgroups. Table 3 suggests similar patterns of response. Were we to stop here, we would not know anything beyond the observation that the patterns of transformed ratings seems to be similar across the different groups. As the subsequent analyses will reveal, our observation might seem correct on the surface, but is wrong. We would not, however, realize that there are deeper patterns, some of which are radically different from each other.

The groups shown in Table 3 are:

  1. Total
  2. Gender (male, female)
  3. Age (18-29 years old, 30-40 years old)
  4. Vignettes rated rapidly (response time < = 3 seconds) vs. rated slowly (response time > 3 seconds)
  5. Mind-Set emerging from clustering (Mind Set 1 of 3 Dealing with the stress of the practice of medicine; M2 of 3 Dealing with difficult patients; MS 3 of 3 Aware of what is important to the patient).

Table 3: Averages of six newly created transformed variables, by total panel and by key subgroups. The averages are computed based upon the original rating assigned to each of the vignettes seen by a member of the subgroup. The numbers can be treated like percentages.

tab 3

Building Models (Equations) Relating the Elements to the Newly Created Transformed Variables

We now move to the heart of Mind Genomics, creating equations which show how each of the 16 elements contributes to the newly created variables. Our analysis will focus on five of the six variables, as we see at the top of Table 4. We will look at the transformed variables corresponding to the highest level of performance (important and remembered), the transformed variables corresponding to ‘important’, the transformed variable corresponding to ‘remembered’, and then the complementary transformed variables of ‘not important’ and ‘not remembered’. The next set of tables will present the parameters of these five transformed variable for each identifiable subgroup previously presented in Table 3. The results will reveal a deeper, far more organized world, one emerging clearly due to the underlying experimental design.

Table 4: Values of the additive constant for five dependent variables (columns) and different groupings of respondents and vignettes, respectively

tab 4

The first step to build the equation is to create the database. The database can be thought of as a rectangular file, one row for each vignette. Recall that each respondent evaluated 24 vignettes, so this database comprises 24 rows for each respondent.

The columns of this database contain the relevant information:

Column 1 – The unique identification number for the respondent. It is sufficient to label the respondents with a sequential set of numbers, starting with 1, and continuing. The study comprises the data from 102 respondents.

Column 2 – Gender

Column 3 – Age

Up to now the data for a specific respondent has been repeated 24 times. We now turn to the data for a specific vignette.

Columns 4-19 correspond to the 16 elements, with each element having its own column. For example, column 4 is reserved for element A1, column 5 or element A2, and so forth. For a single row, each cell (4-19) will be given the value ‘0’ when the element is absent from that particular vignette, or given the value ‘1’ when the element is present in that particular vignette. Since the experimental design prescribe that each vignette will have 2-4 elements, and not more than one element from a question, the database will reflect the design by having the number ‘1’ in two, three, or four columns, and the number ‘0’ in the remaining columns. The rationale for this specific coding, so-called ‘dummy variable coding’ [9], is that the coding enables the regression program to estimate the contribution of each element when the element goes from ‘state =0’ to ‘state = 1’, viz., present.

Column 20 shows the order of presentation of the vignette for a respondent, with the value going from 1 (first vignette) to 24 (last vignette).

Column 21 shows the response time, defined as the number of seconds elapsing between the presentation of the vignette to the respondent and the response. The response time is measured to the nearest tenth of a second. The response time is an important measure in the world of consumer researcher, insofar as it may indicate the presence of different cognitive processes [10,11].

Column 22 shows the rating assigned to the specific vignette by the specific respondent.

Up to now, columns 1-22 were generated by the BimiLeap program, along with data acquired during the course of the experiment. The data can be used for analysis ‘as is’, but consumer researchers prefer to transform the data so that they can investigate different types of answers. There are five specific transformations of interest, focusing on five different aspects of the decision. Each one creates a new dependent variable that will be analyzed in depth.

  1. R5: ‘important’ and ‘memorable’. Ratings of ‘5’ are converted to 100. Ratings 1-4 are converted to 0.
  2. R54: ‘important’. Ratings ‘5’ and ‘4’ are converted to 100. Ratings 1-3 are converted to 0.
  3. R52: ‘memorable’. Ratings ‘5’ and ‘2’ are converted to 100. Ratings 1,3, and 4 are converted to 0.
  4. R12: ‘not important’. Ratings ‘1’ and ‘2’ are converted to 100. Ratings 3,4 and 5 are converted to 0.
  5. R14: ‘not memorable.’ Ratings ‘1’ and ‘4’ are converted to 100. Ratings 2,3 and 5 are converted to 0
  6. To prepare for the analysis by OLS (ordinary least-squares) regression the BimiLeap program adds a vanishingly small random number (<10-5) to each transformed value. The random number does not affect the coefficients of the regression equation, but ensures that the dependent variable will possess some minimal variation across the vignettes for each individual respondent. This variability is necessary for the statistical calculations.
  7. The equation or model is expressed by the simple formula: DV (dependent variable) = k0 + k1(A1) + k2(A2) … k16(D4)
  8. After the parameters of the OLS model for importance (R54) are calculated for each respondent and stored in a second database, that second database to be subsequently used by a clustering program. The clustering program [12], uses the 16 coefficients (k1-k16) as inputs to identify groups of respondents showing similar patterns of 16 coefficients The clustering program assigns each respondent to one of two clusters, based upon similar patterns, and then assigns each respondent to one of three clusters, again based upon similar patterns. The output is the assignment of each respondent to one of two ‘mind-sets’ or one of ‘three mind-sets.’ The assignment is done using strict mathematical criteria. However, the names assigned to the mind-sets or clusters are based upon the elements which are the strongest performers. This criterion is known as ‘interpretability.’
  9. Clustering generates groups of two and three mind-sets, with the name of each mind-set chosen based on the strongest performing elements in the mind-set, viz., the elements with the highest positive coefficients. Taking the three mind-set-solution as an example, Mind Set 1 of 3 is Dealing with the stress of the practice of medicine, Mind Set 2 of 3 is Dealing with difficult patients, and Mind Set 3 of 3 is Aware of what is important to the patient.

    1. Each respondent belongs to several different groups. The first group is Total Panel, viz., everyone. The second grouping divides into the respondent being a male or a female. The third grouping divides into the respondent into younger (ages 19-29) or older (ages 30-40). The fourth grouping divides the respondents by the mind-set to which they belong.
      1. Up to now, the vignettes were assigned to groups based upon the characteristic of the respondent, viz., a gender. We can also look at the vignettes, rather than at respondents to create groups. The fifth groups divides the vignettes by those that were evaluated quickly (operationally defined as response time, <= 3 seconds) versus those that were evaluated slowly (operationally defined as response time > 3 seconds). We can also look at the data focusing our attention on the speed of the response to the particular vignette.

      Steps 1-7 above create a database that is readily analyzed by standard regression methods. The approach here is OLS (ordinary least squares) regression. We begin with the additive constant, k0, shown above as part of Step 6. To review, the equation in Step 6 has 16 coefficients (k1-k16) and the additive constant. The additive constant is a ‘baseline’, defined as the expected transformed value were the vignettes to comprise no elements at all. The underlying experimental design ensures that all vignettes will comprise at lest two elements and at most four elements. The additive constant has no physical meaning other than as an adjustment factors. We can use the additive constant as a ‘baseline’, giving us a sense of the likely percent of responses to be obtained for a given dependent variable (e.g., R54), in the absence of elements.

      6. Table 4 shows the additive constant for each of the groups (rows), and for each of the five dependent variables (columns). For each group and for each dependent variable the additive constants have been shaded for those groups showing an 11 point or higher magnitude of difference between any two members of the group. The large magnitudes of differences in a group are most striking for the three mind-sets, viz., those groups create on the basis of different patterns of coefficients.

      We now turn to the important elements for the five transformed dependent variables, showing only those elements which generate a coefficient of +5 or higher. The standard error for Mind Genomics coefficients varies from 4-6 for most studies with a base size of 100. Furthermore, when elements with coefficients of 4-5 or higher are separately investigated, they often turn out to be relevant to the topic. Consequently, and in the interest of Mind Genomics studies as being early-stage explorations, the cut-off level is set to about 4-5. In this project the cut-off level was set at 5, to follow the conventional practice. In addition, the very strong performing elements are shown in shaded cells, specifically those elements with coefficients of +10 or higher. Finally, each table presents three sets of elements, each set sorted in descending order of coefficient value. The first portion of the table presents the results for relevant elements (coefficient = 5+) for the total panel, gender and age. The second portion of the table presents the results for relevant elements for the three mind-sets. Finally, the third portion of the table presents the results for relevant elements for vignette-based groups (response time, order of testing).

      Mind Genomics generates a great deal of data, much of which can be analyzed in depth for the simple reason that the elements themselves are ‘cognitively meaningful.’ That meaningfulness leads to the ability to discern general patterns, but then to evaluate the nuances of each element.

      7. If we were to summarize the results from the data in Tables 5-9 we would emerge with the conclusion that the strong results emerge from the mind-set, rather than from the self-profiled demographics of the respondents (viz., age and gender), and rather than from the non-cognitive nature of the stimulus (viz., speed of responses, order of testing (first half vs. second half)).

      Table 5: Strong performing elements (high coefficients) for ‘important and memorable, R5’

      tab 5

      Table 6: Strong performing elements (high coefficients) for ‘important (R54)

      tab 6

      Table 7: Strong performing elements (high coefficients) for ‘memorable’ (R52)

      tab 7

      Table 8: Strong performing elements (high coefficients) for ‘not important’ (R12)

      tab 8

      Table 9: Strong performing elements (high coefficients) for ‘not memorable’ (R14)

      tab 9

      Discussion and Conclusions

      Traditionally, the combination of young students and ‘science’ has been to repeat experiments that have been previously performed, experiments whose answers are known. The student’s task is to replicate the experiment in the proper manner, obtain the results, and present the process to the teacher. Success in such a case emerges from the combination of executing the study properly and getting the ‘right answer’. The approach has worked for decades, as generations of students went through the process, some emerging with the feeling that ‘science’ is for them, whereas others emerging with the feeling that this process is not at all for them. A century and more of scientific progress, and the emergence of the modern world with all its technology and ‘know-how’ testifies to the success of the traditional process to learn science.

      The world has changed. One can scarcely spend a day reading newspapers or now more frequently looking at the material flooding forth from the web, to get a sense that the traditional methods of teaching and exciting students no longer work very well. Perhaps it is the ‘tyranny of the small screen’, a phrase author Moskowitz coined to describe the everyday scene of people, young and old, glued to their smartphones. Perhaps the phones are smart, but the people seem to be less smart, or less educated, if one is to believe the ongoing reports in both the academic press and in the popular press, respectively.

      In this emerging world, how then can students be excited? The approach presented in this paper constitutes one way of exciting students through becoming researchers. Happily, there is very systematized thinking about the problems of the everyday, the world where ordinary people live, the world in which they experience the aspects of the quotidian, the daily, the routine. What then could happen if this daily world, so accessible to people, so very ordinary, could be magically transformed into a topic for true scientific investigation, research which not only teaches the student how to think, but actually creates a unique, valuable, indeed absolutely vital corpus of knowledge about the world. After all, the study just reported here, done in just a few hours, from beginning to end, produces unique to the world, valuable information. Most of all, this valuable information comes from the minds of young students, the senior researcher aged 13 (Cledwin), and the junior researcher aged 8 (Ciara), respectively. Their efforts, their curiosity, exploring the topic, produces unique to the world information, the beginning of a large corpus of knowledge on communications to students in the world of medicine.

      There is much to do, more than a billion students around the world, many of them that could be considered the ‘raw material’ of our civilization. Right now the issue continues to be concern about their learning, that they are not learning properly, that their motivation is lagging, that their attention is increasingly on the small screen. Perhaps it is because they are not involved in learning, that learning has become the ceaseless repetition of facts to be ingested, converted to bits of memory, and regurgitated at the proper time, somewhat like the cow chewing its cud. Expressed that way, learning is for the very motivated, and perhaps those who cannot escape this onerous task. But, and this is the important point, what would happen if the students could participate in world-wide projects which create separate topic-specific databases about the everyday, using templated experimental design, and using artificial intelligence. The escape would be into education, not away from education into mindless gaming. One could only imagine a world where thousands of students could collaborate and even compete as they jointly build large scale databases about the minds of people in society regarding the different aspects of daily living. An example might be a large scale database about different aspects of teaching and learning mathematics, across 192 countries, across the entire range from say 3rd grade to end of college, focusing on the many dozens different aspects of teaching and learning mathematics. This effort alone, with 192 countries x 10 aspects of learning mathematics creates a unique, valuable database. One could only imagine the pride of participation and learning for students world-wide who volunteer to participate in this effort. And, to finish, the opportunities are unlimited. Truly in this case ‘the appetite comes with the eating.’

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      2. Kassirer JP (2000) Patients, physicians, and the internet: Coming generations of doctors are ready to embrace new technology, but few incentives now exist to encourage their older peers to do likewise. Health Affairs 19: 115-123.
      3. Murtagh GM, Furber L, Thomas AL (2013) Patient-initiated questions: How can doctors encourage them and improve the consultation process? A qualitative study. BMJ Open 3: p.e003112.
      4. Swennen MH, van der Heijden GJ, Boeije HR, van Rheenen N, Verheul FJ et al (2013) Doctors’ perceptions and use of evidence-based medicine: a systematic review and thematic synthesis of qualitative studies. Academic Medicine 88: 1384-1396. [crossref]
      5. OpenAI (2023)
      6. Moskowitz HR, Gofman A, Beckley J, Ashman H (2006) Founding a new science: Mind Genomics. Journal of Sensory Studies 21: 266-307.
      7. Porretta, S, Gere A, Radványi D, Moskowitz H (2019) Mind Genomics (Conjoint Analysis): The new concept research in the analysis of consumer behaviour and choice. Trends in Food Science & Technology 84: 29-33.
      8. Gofman A, Moskowitz H (2010) Isomorphic permuted experimental designs and their application in conjoint analysis. Journal of sensory studies 25: 127-145.
      9. Suits DB (1957) Use of dummy variables in regression equations. Journal of the American Statistical Association 52: 548-551.
      10. Lenzner T, Kaczmirek L, Lenzner A (2010) Cognitive burden of survey questions and response times: A psycholinguistic experiment. Applied cognitive psychology 24: 1003-1020.
      11. Revilla M, Ochoa C (2015) What are the links in a web survey among response time, quality, and auto- evaluation of the efforts done? Social Science Computer Review 33: 97-114.
      12. Likas A, Vlassis N, Verbeek JJ (2003) The global k-means clustering algorithm. Pattern Recognition 36: 451-461.

Cultural Signifiers in the Subjectivation of Body Image Disorders: The Case of Three School-going Adolescents Addicted to Psychoactive Substances

DOI: 10.31038/PSYJ.2023514

Abstract

The presence of a study poses the problem of the subjectivation of body image disorders in adolescents addicted to psychoactive substances. We start from a clinical observation of schooled Cameroonian adolescents who develop a symptomatology of body image disorders in a sense that is strongly colored by mystical-cultural elements. However, until now, the analysis of the subjectivation of these phenomena has not really been taken into account in the psychoanalytical or phenomenological literature. Based on the theory of cultural relativism of Sow, in particular the theory of conflict relations, we sought to understand the role of cultural signifiers in the process of subjectivation of these disorders when we are under the influence of these substances. To this end, data were collected from three participants or cases, through semi-structured interviews and the DAST-20 test. The results were interpreted in the light of the conflict relationship theory of Sow [1]. It shows that body image disorders are dependent on the breakdown of relational networks, in particular the conflict between the adolescent and the Enemy (witch doctor, sorcerer); the disintegration of his relation to the Ancestor; of the relational rupture between his bio-lineage Family and the Ancestor. The clinical indications of these disorders testify to the impairment of the body, the vital and spiritual principles of the adolescent addict. This implies the objectification of ethno-clinical approaches in the process of detoxification and support for the weaning of addicts, especially school-going adolescents [2].

Keywords

Body image disorders, Teenager, Cultural signifiers, Subjectification, Psychoactive substances

Introduction

The contribution of cultural signifiers in the subjectivation of body image disorders is not taken into account in the scientific literature, in particular the psychoanalytical and phenomenological approaches with regard to their subjectivation of phenomena. Indeed, the image of the body has been theorized by several authors who decline it in several dimensions. Slade defines it as a multidimensional construct that includes two major dimensions, namely: a perceptual dimension and an attitudinal dimension. Price declines it in real body, in appearance body and in ideal body. Dolto speaks of the unconscious image of the body, which he subdivides into basic image, functional image and erogenous image. According to the literature, each of these components is likely to undergo distortions which inevitably modify the perception of the individual vis-à-vis his body, leading to bodily dissatisfaction that the DSM-5 retains in its definition of mental disorders. body image. They are also called body dysmorphia and classified in the nosographic category “obsessive-compulsive and related disorders” and is called “obsession with body dysmorphia” [3-6].

This bodily dissatisfaction is much more noticeable in anorexics, drug addicts and people with a disability or a significant physical condition. In anorexics, from an attitudinal point of view, in general, it is the thighs, hips and stomach that are perceived as having larger proportions than reality. From a perceptual point of view, body image disorder in anorexics is linked to negative judgments made about their own body. From this point of view, anorexics see themselves realistically but are extremely dissatisfied with their body image. The injection leads to a break in the skin for them while those who “sniff” it have perforated nasal septums. Drug addiction or intoxication can also lead to organic damage that alters the physical body with consequences for the subject’s body image and body self-image [7-11].

However, Dolto and Pireyre, starting from the psychoanalytical approach, underline the possibility for an individual who does not suffer from any physical ailment, to develop body image disorders. From the psychoanalytical point of view, the symptomatic elements of body image disorders such as feelings of omnipotence, invisibility, control or fragmentation, etc., refer to archaic bodily anxieties resulting from an absence of symbolization. In infants due to the fragility of the narcissistic foundations. These anxieties reappear in adolescence, which turns out to be an important triggering factor for previous frailties due to the profound changes in the real body of the adolescent resulting in significant psychological repercussions at the level of concrete, imaginary and symbolic reality [12].

At this delicate period of life, the recognition of bodily limits allows a stabilizing image and a protective envelope. Failing this, the adolescent starts looking for strong sensations which give him the impression of rediscovering this physical and psychic envelope, which is why he can feel beautiful, tall, strong, without an objective link with reality. Thus, among drug users, the fragility of the bodily envelope leads to a feeling of emptiness, the absence of bodily feelings, depersonalization, a feeling of fragmentation or of omnipotence [13,14].

These elements, which feed the clinical picture of body image disorders in drug addicts, were listed in the accounts of the three adolescents addicted to psychoactive substances who participated in this study. Their subjectivation of body image disorders that appear in their stories through the clinical indications mentioned above, departs from the psychoanalytical approach as far as the subjectivation of phenomena is concerned. These adolescents, in the sense that they themselves give from their own point of view, to the symptomatic elements of body image disorders that emerge from their discourse, evoke mystical-religious elements such as witchcraft, ancestors, the curse. These imaginary psychic productions highlight the three antithetical doublets of Ego (ancestors, sorcerers, witch doctors) that Sow sets up as interpretative registers in his theory of cultural relativism in general and particularly in his conflict relation theory, to account for the subjectivation of a situation by taking into account the cultural context in which it is inserted.

Thus, these elements of speech of adolescents translate their experience of consciousness in a strongly structured traditional universe which grants an important status to the imagination where hallucinatory images and mediumistic visions orchestrated by the absorption of psychoactive substances, are not only “thoughts more consistent than ordinary thoughts”, but also even more powerful means to access what is hidden but of high meaningful content. This articulation between individual imaginary productions and socio-cultural reality poses the psychism/culture relationship already mentioned by Freud, Roheim, Devereux and others. It is in this ethno-psychopathological perspective that the cultural relativism of Sow fits which, from the anthropological point of view, inscribes, like Hebga, the shadow which is one of the components of the person-personality, as a reliable anthropological foundation to account for the subjective experiences of subjects in relation to spiritual or psychic phenomena which are highlighted in this study, by the mediumistic and psychoanaleptic properties of drugs. In this perspective, the drug ceases to be considered as a simple object or a “fetish” object in the sense of Winnicott, to take on a mystical-religious connotation, since it is part of a pan-structured universe where everything is symbolic [15-18].

Thus, through its psychoanaleptic effects, the drug would act on the shadow to force a disembodiment or stimulate an apparent duplication in which emerge hallucinatory visions allowing the addicted subject to come into contact with cosmic entities, with the only difference that this contact is not facilitated by the state of sleep during which the shadow naturally leaves the body of the sleeper to temporarily join the World of the spirits, or that of the Ancestors, but is provoked by the uncontrolled and unsupervised taking psychoactive substances. It is then that the cultural signifiers inscribe the drug as the determinism of body image disorders, with regard to the syndromy that emerges from the discourse in a culturally symbolic way. Thus, the drug becoming an object of “bewitchment” or a “mediator” of sorcery acted by the deniers of Ego (witch doctors, sorcerers, ancestors), appears as disruptive of the social organization and inducing of psychopathological decompensation.

It is to articulate this link between the psychic productions of adolescents addicted to psychoactive substances and the realities of their socio-cultures that we pose the problem of this study from the cultural relativism of Sow, which is that of the role of signifiers in the process of subjectivation of body image disorders in Cameroonian teenagers addicted to SPAs. The objective of this study is to understand the role of cultural signifiers in the subjectivation of body image disorders in adolescents addicted to psychoactive substances. In order to better understand the psychic dynamics of adolescents, three sub-objectives have been formulated: (1) to understand the intentional action of witch doctors on the biophysiological vital principle leading to body image disorders in Cameroonian adolescents educated addict to psychoactive substances; (2) to understand the intentional action of sorcerers on the principle of life of the existing, leading to body image disorders in Cameroonian adolescents in school addicted to psychoactive substances; (3) understand the intentional action of the ancestors on the spiritual principle leading to body image disorders in Cameroonian teenagers addicted to psychoactive substances.

Method

This qualitative study is exploratory and is based on an ethno-psychopathological perspective. This approach was chosen given that the subjectivation of body image disorders in relation to socio-cultural organizers is an area of research that has not yet been explored. In this context, it is relevant to adopt an exploratory research approach in order to understand the phenomenon by collecting in-depth information on a limited number of participants. Furthermore, this study is one of the first qualitative studies to explore the subjective perspective of body image disorders in adolescents by calling on cultural signifiers. It aims to offer an initial description of these phenomena from the perspective of adolescents in conjunction with the socio-cultural context in which the phenomenon is inserted and gives it meaning. This study is part of an ethno-psychopathological perspective in the natural environment with three Cameroonian schoolchildren addicted to psychoactive substances. Its objective is to understand the role of cultural signifiers in the meaning that adolescents themselves give to the clinical indications of body image disorders that emerge from their discourse.

Participants

The participants of this research are made up of three Cameroonian schoolchildren addicted to psychoactive substances. To be selected, the participants of the study were subjected to the inclusion criteria and the non-inclusion criteria. As inclusion criteria, you had to be a teenager regularly enrolled in a school whose age varies between 11 and 25 years. By considering this age group, we subscribe to the definitional approach of Giedd [19], which extends the age of adolescence to 25 years following the results of research based on the analysis data obtained by magnetic resonance imaging (MRI) of many adolescent brains which show that it is at this age that the brain becomes mature; having been diagnosed as addicted to psychoactive substances or presenting an obvious problem according to the DAST-20 (Drug Abuse Screening Test) with a score ≥ 5. As criteria for non-inclusion, you had to have refused to sign the informed consent; interrupting interviews; being unable to continue the search; have scored ≤ 0 on the Problematic Teen Alcohol and Drug Use Screening Scale (DAST-20) of the body that emerges from their speech.

Material and Procedure

Participants were selected during psychiatric consultations. After a clinical interview and a syndromic record (based on the DSM-V), a diagnosis of addiction was made with the DAST-20 scale which evaluates the degree of severity of drug use ranging from 1 to 20. The score 1 to 5 indicates a low level of problems; a score of 6 to 10 indicates a possibly moderate level of problems; the score of 11 to 15 indicates a substantial level of problems; the score of 16 to 20 indicates the level of possibly severe problems. Adolescents with a score ≥ 5 were offered to take part in the study. Three were selected and agreed to participate in the study after reading the information leaflet and signing the informed consent. The selection procedure that we followed is the non-probability sampling method, which is an empirical method based on a well-considered choice of the selection of individuals by the researcher for the constitution of his sample. The interviews were guided by a grid including themes related to the research questions: 1) motivational factors related to the consumption of psychoactive substances; 2) body image disorders; 3) the psychic dynamics of cultural signifiers; 3) the cultural significance of body image disorders. This research project has been approved by the Ethics Committee for Research on Human Subjects of the Western Region (CERSH-OU) and the authorization to investigate issued by the healthcare firm Angel Gabriel of Bafoussam.

Analyses

We proceeded to a total transcription of the verbatim to not neglect any aspect mentioned by the participant. Afterwards, we proceeded to the coding of the interviews which will make it possible to deconstruct the texts by classifying the quotations by category and illustrating them with the descriptive elements of each person interviewed. Finally, we proceeded to the categorization which consisted in bringing together elements sharing common characteristics according to previously established criteria. The pre-existing categories are linked to the specific themes which relate to the main themes which are: the motivational factors linked to the consumption of psychoactive substances; body image disorders; the psychic dynamics of cultural signifiers; the cultural significance of body image disorders.

Results

The interview with case no. 1, whom we nicknamed “Gildas”, who is 22 years old, a student, single, lasted 27 minutes. He started using drugs when he was 17. Its polyconsumption includes drugs such as tramol, cannabis, alcohol, cigarettes and cocaine. The DAST-20 scale at Gildas showed a score of 16 points, which places him within a positivity threshold of 16 to 20 which reveals a possibly severe level of problems. The interview with case no. 2, whom we nicknamed “Stève”, who is 17 years old, a student, single, lasted 19 minutes. He started using drugs when he was 12 years old and in 6th grade. Its polyconsumption includes drugs such as cigarettes, taï, cannabis, tramol and “pebble”. Passing the DAST-20 scale gave Stève a score of 16 points, which places him within a positivity threshold of 16 to 20, which reveals a possibly severe level of problems. The interview with case no. 3, whom we nicknamed “Jonas”, who is 23 years old, a student, single, lasted 23 minutes. He started using drugs when he was 18. Its polyconsumption includes drugs such as cannabis, alcohol and cigarettes. His DAST-20 test score resulted in a DAST-20 test score of 11 points, which puts him within an 11 to 15 positivity threshold that reveals a possibly substantial level of problems. The analysis of the various speeches collected from each of the three participants confirms the presence of body image disorders which are expressed, among other things, in feelings of invisibility, invincibility, transparency, psycho-corporal emptiness, depersonalization, derealization., fragmentation, right of way or possession.

Depersonalization

Depersonalization is explained by the fact that the subject does not have the impression of being inside his body, nor of the world. The interface between the inside and the outside of the body is not delimited. She expresses herself in Gildas with words like: “It’s like an oppression (…) Once it’s the chest which seemed as if something moved inside… Afterwards you act as if you’re in the machine, as if you were in pieces. For Stève: “You feel as if someone is living in your body. For Jonas: “It makes us do things we didn’t expect, as if you were another person. “I thought I was above, so above that I turned into a glider”.

The Morsels

The morsels refer to the impression that his body is not unified. It is the effect of depersonalization that modifies the bodily experience leading to the subject, anxieties of fragmentation, bodily transformation, annihilation or division. Fragmentation becomes a disturbance of the image of the body insofar as the anxiety of fragmentation is a threat to the cohesion and continuity of the subject by breaking into pieces or bursting. Because of fragmentation, the individual cannot experience a stable identity. The subject is little differentiated from the object. It is translated by Stève in these terms: “I have the feeling that my body is divided into pieces. Bion (n.d.) quoted by Goyty [20] describes fragmentation anxiety as “emptying anxiety”. This feeling of emptying or psychocorporal emptiness is translated by Jonas when he says: “You no longer feel your body”.

The Derealization

Derealization is a state of consciousness or an altered perception or experience of reality that appears dissociated or external to oneself. Derealized people sometimes say that they feel unreal or like an automaton, with no control over what they do or say. This sense of derealization is evidenced in Gildas by lyrics like:

It’s as if I saw far from me. I also saw the little ones there, the guys were scaring me on the way. I even wanted to slap them… It’s as if you have to deal with the same person everywhere. When you talk to this one, he just laughs. Afterwards, you feel that it is not this one that speaks to me. You want to change, you feel it’s weird. Can you see a little?… Afterwards, you see, you have money easily (…) The eyes were turning as if it were going behind your head to get you to see things (…) Someone is telling you now, you mustn’t cheat on your wife, when you have a wife like that, like that. We finish talking to you, you leave. A woman comes out of nowhere and tells you she loves you. You are overwhelmed, you say shit to yourself. What is that.

For Jonas: “You do things without understanding…You have the impression of flying away, of speaking with spirits. For Stève: “It’s as if I was flying away. It feels like my feet weren’t touching the ground anymore.”

The Feeling of Being Under Control

Control or possession refers to the state of a person who is in the power of a demonic power. It can be done by bewitchment by means of objects or mediators such as food, animal vectors, instruments-amulets. This feeling of being under control is manifested in the subject by the impression that his body no longer belongs to him, that someone else is carrying his body or remotely guiding him. This feeling of being under control is translated in Stève by: “I tell myself that there is a spirit behind which controls me…I sometimes felt as if someone possessed my body. I do things without knowing and when I do, I find that what I did was not right. Right now, it’s not me who’s doing it.” For Gildas: “I have the impression that my body no longer belongs to me, that I do things as if someone was controlling me. Sometimes, in class, I heard a voice telling me “go out and have a smoke”. It was then that I left school to find myself in the neighborhood with friends. For Jonas: “Some say it’s bewitchment… As if a spirit was inhabiting you and pushing you to do things”

The Invincibility

Invincibility is expressed by the feeling of narcissistic omnipotence which gives the subject the impression of divinity. This feeling of omnipotence is expressed in Gildas by words like: “I could say that I cross the portal without the supervisor seeing me and I cross he does not see me…Even if there is a wizard in the neighborhood, I know that he cannot do anything to me because I am powerful”. For Jonas:

I felt invisible, super powerful. We believe we are capable of anything. When you take this, you change dimension. You think you are powerful. It puts you in a mind where you believe that you are capable of anything, that nothing can stop you. In my hallucinations, I saw life in a different way. I thought I was above, so above that I turned into a glider. I even had to forget who I personally was. I was like a god since I minimized or marginalized those around me.

Steve is of the same opinion when he says: “You are strong. You speak, everyone listens. You become like god”. The subjectivation of the participants in the study in relation to these different elements calls up the cultural signifiers available in the sociocultural field. This meaning given to the elements of body image disorders by adolescents addicted to psychoactive substances, will be apprehended from Sow’s theory of cultural relativism and in particular, his theory of relationship conflict.. Indeed, Sow postulates that all phenomena can only be understood in the socio-cultural context in which they emerge and which gives them meaning. It is in this perspective that he inscribes the mental disorder as the expression of a conflict between the individual and his relational poles, because in Africa, the sick subject is always the victim of an aggression external to him whose aggressor or denier of self or identity, can be the sorcerer, the witch doctor and the ancestor. In the context of this study, all these negative doublets of Ego use the culturally inscribed drug as a determinant of body image disorders because of its psychoanaleptic effects on the shadow or the ego of the subject who immerses his functionality in a hallucinatory dimension that is similar to psychic or paranormal phenomena that emerge from the discourse of study participants in a culturally symbolic way.

The Conflicting Relationship between the Ego and Sorcerers/witch Doctors

Sow thinks that mental disorder is “Ego ‘violated’ by the rupture, caused by an aggressive otherness, of one or other of its links, stemming from the fundamental constituent poles” (p.30). In this study, the first constituent pole is that of Ego and the sorcerer. All the participants in the study mention witchcraft as a possible explanation for the clinical indications of body image disorders that emerge from their speeches due to the mediumistic and psychoanaleptic properties of psychoactive substances. We can underline it in the student Jonas who says: “We say to ourselves that there is something behind. People of bad hearts, one mind. In the same perspective, Gildas affirms: “Sometimes they are said to have been sold into witchcraft or that they are driven by a spirit or by bad people. “For Stève:” But I think that this thing is not clear. There is something behind. Maybe witchcraft, spirits or an invisible hand”.

These different subjectifications of the elements of body image disorders given by the participants in the study, which underline the action of the horizontal antithetical doublet on Ego, highlight the importance of the etiology of the phenomena still considered in Africa as an indicator of disruption of social relations [21]. In the context of the study, the drug is not just a simple herb or profane object, but is considered a magic herb possessing powers which, however, are not innate to it, but are dependent on the antithetical doublets of ‘Ego who use it to reach their victims. This is noticeable in the account of study participants who speak of being “driven by a spirit or bad people.” As for Jonas, he is convinced of this mystical-religious character of psychoactive substances when he says: “In fact there is always a spirit in the matter there because as soon as you touch that, everything changes. Gildas continues along the same lines when he says:

Some will perhaps talk about the action of wizards, or will say that it was out of jealousy that someone would have done this. But I think there is a force behind drugs. It’s not simple. Because in the sect, we consumed it to get in touch with the grandmaster. I even distributed it to some young people to introduce them to our sect and when I met them later, something in me told me that they were already part of our number.

This account by Gildas accounts for the correlation existing between psycho6active substances and magico-religious practices or even between psycho6active substances and consumers who become people apart, sometimes endowed with supernatural and premonitory powers. This is noticeable in Steve as in most of the participants in the study, when he says: “You become invisible. It’s like you think something and that’s the only thing that comes. You become like a prophet. Anything you want, you get. You have the money without knowing how you do it. You don’t have the money but when you want the money, the money comes. So Jonas thinks it’s “real witchcraft.” The conflict that is expressed in this case is not psychic or internalized like a breach of the psyche, since it is located outside of the drug addict. It is a dynamic conflict which is situated, analyzed and actualized in the relations of the subject with one of its constituent poles which is an antithetical doublet in the rank of which the sorcerer or the witch doctor. It is therefore by design that the notion of conflict is inscribed in African psychopathology as the interpretative base of psychic phenomena whose aggressor agent is a negative doublet of Ego in the sense that it deconstructs Ego in its constitutive structure that is the person-personality. It is in this sense that Stève recounts his first experiment:

The first time, it is as if the plane had hit me. Everything vibrated in me. Because there are people who do like that there it takes them they die once. His pressure is raw, very raw even. Me when it took me hmmm. I was already screaming that I’m already finished. I was saying in my head that I’m already dead. The day there I said that I can no longer take. It is as if my soul came out of my body. But then I always took. There is witchcraft behind.

Along the same lines, Gildas describing his experience, says:

I feel like my body doesn’t belong to me anymore, that I do things as if someone is guiding me. Sometimes, in class, I heard a voice telling me: “Go out and have a smoke.” It was then that I left school to find myself in the neighborhood with friends… I not only heard voices, but I also saw the leader of the sect who often appeared to me to warn me or give me instructions..

As noted by Gildas, the persecuting violence that his shadow-soul undergoes is exogenous. For Sow it is this persecutory violence suffered by Ego and conceived as external to him, which allows Ego’s psyche to maintain and reinforce its internal coherence. Thus the notion of conflict relationship is an important psychological modality in Black Africa on the triple plane of the imaginary, the real and the symbolic. For Sow, the interpretation of the mental disorder requires that the persecuting external agent be identified, which also amounts to identifying the problematic dimensional axis of the attacked subject who, in the context of the study, is the adolescent addict to psychoactive substances. It is this approach that the parents of Silas opted for by consulting Nkamsi or witch doctors: “They went. The seers said it’s the drug. But that there is a spirit behind that people use to harm me. Thus, the psychocorporal disorder in relation to the psychoanaleptic effects of the SPAs which the student Silas faces, has as its conflicting pole, a spiritual entity.

We note here the importance of the imagination or the symbolic representations that furnish the psychic functioning of the addict subject. These representations concern the conflicting dimensional axis which is characterized by an enemy (spirit) who uses the drug as a fetish or a “mediator” because of its psychoanaleptic properties, to attack Ego in its shadow. The similarity of the content of the participants’ speeches makes it possible to realize the constituent background of the individual imagination in highly structured traditional African societies. These constituent resources of the individual imagination, although they vary from one pupil to another, do not present significant differences, despite the fact that the conflicting pole can vary from one pupil to another according to the frailties individual and socio-cultural determinants.

The Conflicting Relationship between the Ego and the Ancestor

In the African cultural imagination, the Ancestor or the ancestral signified is the founder and guarantor of the law, rules, principles or prohibitions. The respect of these laws and prohibitions, guarantees the development of the individual within the cultural community in which he derives his existence, because, outside this community governed by the law of the Ancestor, he is considered as a non-be. Thus, the entire existence of the individual is governed by these laws, the violation of which has consequences on all the existing relational poles. It should be emphasized that in Sow, the horizontal and ontogenetic dimensions are maintained and consolidated by the links from the major Pole of ancestral verticality. The set of all these relations constitutes an ordered totality, founded on and guaranteed by the Being of the Ancestor located at the top of the African cosmogonic pyramid.

However, the parents of Steve, Jonas and Gildas seem not to subscribe to this system of beliefs because of their Christian faith. Steve says: “They went. The seers said it’s the drug. But that there is a spirit behind that people use to harm me. That there are sacrifices that must be made because the ancestors are angry. Things like that. But my mother didn’t believe that. She is a believer. In the same perspective, Gildas, whose parents are Christians, says: “I have already learned that some seers say that it is the ancestors who are angry, perhaps because the parents of the victim did not perform certain rites.. For Jonas: “Some say it is bewitchment, a curse (ndô) and that the spirits of the ancestors are angry. “The curse called “ndô” in Bamileke country, is always the expression of the anger of the ancestors because of the transgression of a prohibition or the neglect of an important rite. The victim may not be directly affected by the transgression or neglect in question, but suffer from the faults of the parents. In this case, we speak of a “designated patient”. This shows that in the African cultural imagination, all acts and omissions have consequences.

Indeed, the transgression of the Word, of the Law of the Ancestor generates the rupture at the deep level of the cultural link. This transgression leads to a state of disorder, heartbreak and insecurity at the individual and collective level. Because, from the cultural point of view, the founding Ancestor of the clan or lineage is the only one who can totally and fully guarantee the physical and mental well-being of the subject. As much as respect for its law guarantees cohesion between the constituent dimensions of Ego, their transgression disrupts the harmonious relationship through its direct action on each of the constituent dimensions of Ego. Also, in traditional African thought, the vertical antithetical doublets of the self, such as wandering spirits and geniuses who had privileged relations with the Ancestor, are likely to influence the deep being of the subject and disorganize as much as the Ancestor, his relational networks. They can only be calmed by skillfully performed rites [22]. This is apparent from Silas’ account when he says, “The seers said it’s the drug.” But that there is a spirit behind that people use to harm me. That there are sacrifices that must be made…”.

Thus, given that in the collectivist culture, the individual has no life of his own apart from that of the community which defines his existence and his identity, any member of the collegiality who transgresses the taboos or refuses to submit to the law of the Ancestor, which ensures the cohesion and harmony of the group in the cosmic order, incurs the most severe sanction of the Ancestor, the Genii or the spirits who are attached to it [23]. In this study, the said sanction comes in the form of a mental disorder caused by the consumption of SPAs. This is the case of Jonas received in psychiatric consultation for acute delusional puffs with hallucinations. Apart from the transgression of an ancestral prohibition that the therapist must identify, or the refusal to perform a rite by the parents which would explain the mental health of the addict subject, the drug in itself constitutes an existential threat, since its use is part of the antithesis of ancestral principles and whose sanction can be collective because of the collective solidarity which fundamentally characterizes traditional societies. Thus, we realize to what extent the violation of a prohibition by an individual not only affects his relationship with the Ancestor, but also generates disorder in the relationship between the bio-lineage family and the Ancestor.

The Relational Disorder between the Bio-lineage Pole and the Ancestral Pole

The cosmogonic triangle of Sow, places the Being of the Ancestor at the top of the pyramid with at the other ends the bio-lineage family and the cultural community. The set of all triangular relationships constitutes an ordered whole, founded on and guaranteed by the Being of the Ancestor. Thus, apart from Ego, the vertical axis also articulates the Ancestor with the unity of all: individuals, family, lineage, clan between them on the one hand and, on the other hand, of all with the whole of the culture. This axis confers on all the “Existing” the fundamental dimension and consolidates their phylogenetic continuity on the triple level of Being, Existence and Culture. The vertical axis can be disrupted due to “outside the contract” [24] behavior of one or more family members. In this perspective, when the seer consulted by Silas’ parents says “that there are sacrifices that must be made because the ancestors are angry”, this implies that Silas is only a victim or a “designated patient”, the ancestors having just used it to express their anger vis-à-vis the family or the cultural community. In this perspective, the fault committed by an individual becomes systemic or collective. In this case, reconciliation with verticality requires an “oblative gesture of a sacrificial victim” which re-establishes the cohesion between the different constituent poles of the person-personality. This is what Silas’ story refers to when he says that “there are sacrifices that must be made”.

The disorganization of the ancestral relationship with the bio-lineage family can manifest itself directly or spread over time. This is what Stève points out when he says that: “Sometimes it is faults that were committed by ancestors that my parents themselves did not know. This story, which results from the diagnosis made by the seer on the etiology of Steve’s addictive behavior, reflects the resentment of the ancestral meaning that requires reparation so that misfortune moves away from the family, at least for the moment. Because the relationship with verticality remains fragile with the tellurian posterity because of their problematic behavior. Thus, the ancestor, because he is the reference, the ultimate recourse of the person-personality because he is the founder of the community and of the current cultural order, remains the veiled meaning of the mental disorder. of an individual who is in the context of this study, body image disorder in school-going Cameroonian adolescents, due to the psychoanaleptic properties of drugs.

In this context, the psychocorporal modification of the body due to the magico-cultural properties of drugs, is a warning and at the same time, a call to a prompt repair of a fault committed. It is a violence exerted on Ego which indicates a threatening symptom of the cosmic order and the communal balance. The disorder is always expressive of an exogenous violence that fundamentally calls into question the cultural relational structures, created by the Ancestor, which guarantee the place and security of all in the cosmic order. This challenges the members of the bio-lineage family of Silas, Jonas and Gildas to reconsolidate their disturbed relationship with the Ancestor. Thus, according to Sow, the traditional interpretation of phenomena foresees or classifies the different causes of conflicting disjunction of individuality, according to its constituent dimensions or constituent axes, as so many substantiated antithetical “doublets” of the constituents of the self. These constituent dimensions which are external to the individual, can only function in close articulation with the constituent elements (body, vital principles, Spirit) of the intimate structure of the person-personality of the individuality whose aggression is manifested by specific clinical signs, makes it possible to identify the latent signified which is more important than the objective clinical elements. This is how Devereux and Sow think that reflection on the psychological meaning of individual and culturalized psychic productions which are lived experiences of the subject’s consciousness, as manifest symptomatic material, must be articulated with the socio-cultural reality that sheds light on its significance and gives it meaning.

In this ethno-psychopathological study, the adolescent addict to psychoactive substances is culturally and theoretically considered as a person-personality. So, the symptomatology of the disorders of the image of the body which emerges from his speech as well as the meaning that he gives to it, must be grasped from the ethno-psychopathological point of view, as an attack on the constituent elements of the person-personality., namely: the biophysiological principle, the vital principle and the spiritual principle.

The Biophysiological Vital Principle or the Life-Soul

According to Sow, the aggression undergone by Ego on its biophysiological principle by the horizontal antithetical doublets of the self that are the marabouts/fetishers, causes psychosomatic affections among which: the state of social inhibition, fatigue physical, mental fatigue, sexual disorders, dreams of persecution, etc. These clinical indications of psychosomatic disorders were found in the participants in this study. Student Gilda expresses the achievement of her biophysiological principle in these terms: “You get up in the morning, you take. You don’t have the strength. You don’t want to do anything. You just want to sleep… You have a stomach ache, appetite disorder. Sometimes there are noises. You can’t sleep. If I go to bed at 8 p.m., even at two o’clock I will still be awake. My head would only be, it’s just spinning. Even the woman doesn’t say anything to you anymore. You have sexual weaknesses. In Stève, his syndromic summary retains insomnia and migraines. He himself recounts his first experience in these terms: “My neck hurt. It hit, it hit, it hit. Then you act like you’re in the machine. Like you’re in pieces. Once it was the chest that seemed as if something was moving inside. In Jonas, we note asthenia, insomnia, amnesia, tremor, dizziness, headaches, tachycardia, anorexia, etc. These syndromic summaries testify to a dysfunction of the biophysiological principle which results from the violence of the Enemy on Ego. This suffered violence, persecuting Ego, is orchestrated by a persecuting agent or an aggressive otherness which is outside of it and which allows the psyche of Ego to maintain and reinforce its internal coherence. Thus, for Sow, the persecutory object relationship is a banal psychological and psychosocial modality in Black Africa, on the triple level of the imaginary, the daily reality and the symbolic universe. Because, in the culture and the effective social praxis in Africa, these different objects of the activity of the psyche are not distinguished from each other, but maintain between them, a relation of continuity.

For Sow, this object relationship is expressed at the level of experience, essentially through themes such as possession, influence, devouring, etc. The story of the student Stève translates this experience well when he says that “there are times when I am smoking, I tell myself that there is a spirit behind that controls me… I sometimes felt like if someone owns my body. I do things without knowing and when I do, I find that what I did was not right. Right now, it’s not me who’s doing it.” For Jonas, “in fact there is always a spirit in the matter because as soon as you touch that, everything changes and it then awakens certain spirits in you which are not convenient, that’s a bit like that. Some say it’s bewitchment.” These different accounts of the experience of consciousness of students addicted to SPAs not only testify to the existence of a “structured imaginary cultural collective”, but also provide evidence of an attack on the biophysiological principle by an aggressive otherness. whose acts of destructive aggression do not spare the vital principle of the existing.

The Vital Principle or the Soul-Shadow

The vital principle or the soul-shadow is the internal principle which caps the biophysiological principle. It presides over the existence of individuality. Its role is to enliven the existing. Thus, life, strength and fertility are under his control. For Sow, the impairment of the principle of life of the existing is expressed through the following clinical signs: the feeling of being literally emptied, pumped of its substance; the symptomatic procession accompanied by a feeling of imminent death, with physical signs such as: palpitations, dry mouth, agitation, etc. We find these symptoms in Gildas whose syndromic summary retains palpitations and agitation. The student Stève is part of the same logic when he says: “I had the impression that my body at some point no longer belonged to me. It is the same for Jonas who says: “You no longer feel your body. “All of these lived experiences of consciousness reveal the disintegration of the life principle of the participants in the study. For Sow, this disintegration induces paroxysmal, acute anxiety without temporo-spatial destructuring, but with the feeling of imminent death, as can be noted in the student Stève: “I was already screaming that I am already finished. I was saying in my head that I’m already dead. For Jonas, “you no longer feel your body. Real witchcraft what… Some say it is bewitchment”. In fact, bewitchment is an operation by which one strikes a substitute for the targeted person (effigy, nails, hair, linen, etc.) with blows that are supposed to reach him.

In the absence of appendages (nails, hair, etc.), the sorcerer uses mediators which come in the form of food, animal vector, instrument-amulet to bewitch his victim or to gradually destroy his vital potential. He conveys the soul of sorcery in his acts of destructive aggression. The mediators through which the sorcerer conveys the soul of witchcraft include all the elements of nature in an environment where the sacred is omnipresent, a pan-structured universe. Sow calls these elements of the cosmos “revealing clues to the divine”. For Zahan [25], the environment here includes all the elements of nature that are in direct contact with man, from his body linen to the cosmos in all its complexity, including the soil with all its content (plants, minerals, living beings, all patrimonial assets). Drugs are part of this “patrimonial asset” or of these “indicators revealing the divine”. In traditional societies, it is used in mediumistic practices or for magico-cultural reasons because of its psychoanaleptic properties, as is the case with initiation into bwete among the Mitsogho of south-central Gabon. As a result, it can, beyond mediumistic visions and provoked and controlled hallucinatory images, serve as an “instrument-amulet” in which the sorcerer conveys the soul of witchcraft to harm his victim. This instrumentalization of drugs is reflected in the words of Gildas when he says: “But I think there is a force behind drugs. It’s not simple. Since in the sect, we consumed it to get in touch with the grandmaster ».

Always to translate this magico-cultural or mediumistic character of drugs, Stève says that “there are times when I am smoking, I tell myself that there is a spirit behind that controls me…It is true that people are going to say that I have been sold into witchcraft or that it is the spirits who want to destroy me”. As for Jonas: “You suddenly become powerful, as if a spirit were inhabiting you and pushing you to do things. You no longer feel your body. Real witchcraft what… in fact there is always a spirit in the matter because as soon as you touch that, everything changes. The experience of bewitchment by the drug revealed by the psychic productions of the participants in the study, accounts for the investment of the vital principle by the doublet of the self or the negator of existence which penetrates to the depths of the Being and Existence of the person-personality.

The Spiritual Principle or Soul-Spirit

The spiritual principle is the quintessence of the psychic life of Man. It is a spiritual substance made of intelligence and will which is indestructible and imperishable. It is in close relationship with the ancestral pole or the ancestral signified which is the only one to access the spiritual principle of the Ego. This principle is affected when the relationship of the Ego to the Being of the Ancestor becomes problematic. It is reached when there is a conflict with the most fundamental Tradition, that is to say the Being, the Word and the Law of the Ancestor. For Sow [2], the achievement of this fundamental component of the individual leads to the following clinical indications: a state of agitation with experience of confuso-oneiric consciousness, incoherent remarks, denudation, as well as a strong feeling of to be influenced, to be possessed. These symptoms evoke first of all problems with the vertical dimension which includes: the Ancestor, the genius, the Wandering Spirit. The inferred point of impact is the “ontological” dimension of the subject. This is the typical case of the acute psychotic outburst, which was noted in Jonas whose syndromic summary indicated acute delirious outbursts with hallucinations. It will emerge from his speech that his parents are Christians. Their religious affiliation is at odds with traditional beliefs, which creates relational difficulties between the Ancestor and the family or between the Ancestor and the Ego used as a victim. This is what emerges from the words of Gildas when he says: “I have already learned that some seers say that it is the ancestors who are angry perhaps because the parents of the victim did not perform certain rites… I had a friend, the kamsi had said with regard to his case, that the ancestors were angry because he had refused to subscribe to the ritual required to be Nkamsi”.

The symptomatology which reveals the attack of the spiritual principle includes not only the syndromes of the biophysiological principle and those of the vital principle, but also psychotic decompensations such as depersonalization, derealization, the feeling of being under control. The depersonalization is noticeable in the student Jonas when he says: “It makes us do things that we did not expect, as if you were another person. For Stève: “I have the feeling that my body is divided into pieces. As for Gildas, he translates this feeling with words like: “It’s as if I was flying away. Looks like my feet weren’t touching the ground anymore. Possession is evidenced in Gildas through the following account: “I feel like my body no longer belongs to me, that I do things as if someone is guiding me. Sometimes, in class, I heard a voice telling me “go out and have a smoke”. It was then that I left school to find myself in the neighborhood with friends. As for Jonas, he puts it this way: “It’s as if a spirit was living in you and pushing you to do things. Also, the hallucinations caused by intoxication with psychoactive substances in Jonas, which are unusual experiences of consciousness, far from being pure “regressive” and narcissistic chimeras, correspond, rather, to what Sow calls, a surreality. For him:

If Psychoanalysis as well as traditional culture make the content of the imaginary a manifest symptomatic material, referring to a latent structure which founds it, then clarifies its meaning and gives it meaning, the articulation of the contents of the “imaginary “traditional with the elements of socio-cultural reality seems to us better established and more coherent insofar as, not only does traditional society give a valued and privileged status to the imaginary (access to something else: the community signified articulating the verb of the ancestor who goes beyond and founds the singular subject) but, moreover, because the traditional values, as coherent with respect to the cultural link, make it possible to link, in a more satisfactory way, the links of meaning with a structuring principle for the set of subjects: the Ancestor. (p.63).

According to Sow, the hallucinatory vision which is an experience of lived consciousness, must be considered as a manifest clinical or symptomatic material which reveals the deep layers of the human psyche thus giving access to the fundamental signified which is the Ancestor. Thus, the hallucinatory vision or the state of trance-possession cannot have the same status as in Western psychiatry, quite simply because their structure, their meaning are absolutely different, proceeding from different anthropological Totalities. It is on purpose that intoxication with psychoactive substances, which resembles a “spontaneous” or “wild” psychotic experience, because it is not controlled by the usual rules, leads to psychotic decompensations (schizophrenia, melancholic, psychosis obsessive, etc.) which are serious mental illnesses because they proceed from the most intimate disorder in the Being of the Existing. It is essentially interpreted as resulting from a conflict with the dimension of verticality. This explanation is confirmed by the friend of Gildas considered “crazy” for having refused to subscribe to the rituals to become Nkamsi. All the “imaginary” productions of the participants in the study in relation to their subjectivation of phenomena are “uncovering” the deep layers of their human psyche which are part of the systems of thought and the real social practices of their cultural group. These are personal experiences of consciousness in traditional Africa that highlight the existence of a “structured imaginary cultural collective”. We can therefore say that the individual imagination literally draws from community praxis and ideology the essential intellectual elements of its mental elaborations. Because, all the stories of the participants as lived experiences, are made according to the cultural signifiers available in the social field in which they are inserted.

Discussion

The analysis and interpretation of the speeches of the participants in the study show that they give an important place to cultural signifiers through mystical-cultural elements. We note the occurrence of the words “witchcraft”, “spirit”, “ancestors”, etc., as an attempt to explain the paranormal phenomena that appear in their speeches and that they seem to have experienced under the effect of psychoactive substances. This meaning that the study participants give to the clinical indications of body image disorders that emerge from their accounts, is culturally embedded in Sow’s cultural relativism, in particular his relationship conflict theory which defines illness as a violation that disarticulates the Ego as a constituent reality (body, vital principles, Spirit) with its different constituent dimensions (ancestral meaning, biolineage family, socio-cultural community). The aggressor agents who are the deniers of Ego, are culturally signified as witch doctors, sorcerers and ancestors. The various pathologies which result from the aggression of Ego on its various components, are culturally indexed and are declined in psychosomatic affections due to the intentional action of the fetishers and the marabouts who disorganize the biophysiological principle or the soul-life; the morbid disorders which are due to the intentional action of sorcerers who disorganize the vital principle; the most serious morbid disorders which are due to the intentional action of the ancestors which disorganizes the spiritual principle.

In this study, the elements of culture evoked by adolescents in their discourse, include drugs as the determinism of body image disorders, with regard to the syndromy that emerges in a culturally symbolic way. Thus, drugs, which are part of the sacred in a pan-structured universe, appear to disrupt cultural organization and induce psychopathological decompensation. In his relationship conflict theory, Sow inscribes in the register of morbidity, fragmentation, depersonalization, the feeling of emptiness, etc., orchestrated by self-deniers on the vital principles of Ego (soul-shadow, soul-spirit). These clinical indications emerge from the discourse of adolescents as characteristic signs of body image disorders resulting in the feeling of having a split, transparent or bewitched body, etc.

For Hebga, these clinical indications, which translate the culturalized experiences of consciousness of adolescents as well as the meaning that they themselves give to them according to their own points of view, account for the paranormal phenomena that he explains from of his triadic theory which structures the human being into three components namely: the body, the breath and the shadow. He thinks that it is through the shadows that bewitchment, bewitchment, apparitions, visions, action at a distance, etc., in short, so-called paranormal phenomena are made possible. The shadow in Hebga is the parallel of what Sow calls “vital principle” or “soul-shadow”. It is the seat of individual psychic life and therefore the basis of paranormal phenomena. In the logic of Sow [2], it can suffer the effect of any immaterial intrusion that can immerse its functionality in a hallucinatory dimension that is similar to what Hebga calls paranormal phenomena.

In this anthropological, vitalist and dynamic perspective of phenomena, the drug through its psychoanaleptic effects would act on the shadow to facilitate the emergence of hallucinatory visions, thus allowing the addict subject to come into contact with spirits, with the only difference that this contact is not facilitated by the state of sleep during which the shadow naturally leaves the body of the sleeper to temporarily join the World of the spirits, or that of the Ancestors, but is provoked by the uncontrolled taking and unframed psychoactive substances that act on the shadow to force a discorporation or stimulate an apparent duplication. This apparent duplication is due to the power of the plant which, by its magico-religious and/or mediumistic property, highlighted by shamans in their traditional therapy, not only allows “access to the sacred world, but participates in the sacred especially when one is in a cultural context where the sacred is omnipresent through natural elements. Thus, the power of the drug is transferred to the subject’s body, investing his vital principles (soul-life, soul-shadow) as well as his body image to give him the impression of “flying away”, of ” to split” or “to be under the influence”.

For Hebga, on the psychopathological level, addictive consumers of psychoactive substances manifest the same clinical signs as those found in patients who are under the influence of bewitchment and who go through paroxysmal crises in which the fear of dying and where many somatic manifestations appear: respiratory and cardiac problems, gastric spasms, feeling of imminent death. The person convinced of having been sold, lives in the obsession of this diabolical market. He has the feeling of being split or of being emptied of his vital substance. His invisible self works away from his visible self. One could suspect here the feelings of depersonalization and derealization which are present in schizophrenia or melancholia and which are also found in people addicted to psychoactive substances.

According to Hebga like Sow, we can say that when teenagers addicted to SPA talk about witchcraft, ancestors or spirits to explain these clinical signs of body image disorders that emerge of their stories such as the feeling of depersonalization, of fragmentation, of psychocorporal emptiness, etc., they do not tell chimeras, but describe a phenomenon which basically corresponds to a traditional milieu, to something transcribable, decipherable. It is in this perspective that Nguimfack insists on taking into account the cultural material brought to the clinic as being first and foremost, psychic material. For him, the patient lives with this material on a daily basis and it guides his behavior, his thoughts and his vision of the world. While giving a special status to the cultural material brought to the clinic by the patient, he says that “if someone evokes witchcraft, beliefs or the curse to explain his miseries or even his postures in the face of them, it is, for him, of an element that culturally structures his psychic functioning at that time” (p.117).

This taking into account of culture in the understanding of disease developed from the work of Devereux, the originality of which lies in the understanding of people’s suffering in relation to their beliefs, their customs, their traditions, their history, in short their cultural meanings. By adopting such an approach, Devereux draws inspiration from these predecessors like Freud and Roheim to extend the discussion on the influence of culture in psychic construction. Beyond the universality of the unconscious that Devereux admits, he demonstrates that every human being is imbued with his culture.

Conclusion

The absence of a non-containing environment brings back the archaic problems in adolescence by modifying the experience of the subject’s bodily limits causing feelings of loss of body containment, loss of body content, which alters the function of the Ego-skin, thus pushing the individual in search of strong sensations which give him the impression of finding this physical and psychic envelope. He feels beautiful, tall, invincible, invisible, under the influence or even under the impression of freeing himself from existential constraints and finally mastering his destiny. However, these psychoanalytical and phenomenological explanations of the clinical indications of body image disorders, which constitute significant addictive problems, are not to the taste of the adolescents addicted to psychoactive substances who participated in this study. Their subjectivation of body image disorders based on clinical indications noted in their speeches, highlighted the cultural signifiers specific to their socio-culture. Their evocation of witchcraft, ancestors, curses or spirits to account for the disorders of the image of their bodies, testifies to their lived experience of consciousness or their imaginary production which, despite their individuality, is imbued with collective imagination in a panstructured universe where the persecutory object relationship is a psychological and psychosocial modality on the triple level of the real, the imaginary and the symbolic world which manifests itself at the level of experience essentially through themes such as possession, influence, devouring, depersonalization, derealization, etc.

This content of consciousness or culturalized material which explains the disorders of the image of the body by the achievement of the constitutive principles of the person-personality which disarticulates Ego with its constituent poles (vertical, horizontal, ontogenetic), must be articulated with reality. socio-cultural. Thus, the questioning of deep anthropological structures is likely to generate a state of ill-being in an individual, in his family and in the entire cultural community, since there is interweaving of links at the functional level between the structure of the person-personality of the adolescent addicted to SPAs and those of socio-cultural relations. It is in this sense that Nguimfack makes cultural material, clinically significant psychic material, since not only does it influence the vision of the world of the SPA-addicted adolescent, but also accounts for his psychic functioning in this moment.

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Chronic Psychogenic Hyperventilation, Hypocapnia and Metabolic Acidosis: Hypoxia, Inflammation, Aging and Age-Related Diseases

DOI: 10.31038/ASMHS.2023712

Abstract

In this review article, author discusses about the biomedical importance of carbon dioxide in regulating the cellular homeostasis. Even more important, the author had already presented a hypothesis regarding the role of carbon dioxide in regulating cellular homeostasis, which was published in the program and abstracts (serial number 237) of 1st world congress on stress held in USA, October 4-7, 1994. The author goes even further to explain how chronic psychosocial stress and unhealthy diets are the two key risk factors for developing hypocapnia, low-grade metabolic acidosis and insulin resistance, which, in turn, has been shown to predict the development of age-related diseases, including hypertension, coronary heart disease, stroke, cancer, and type 2 diabetes. The writer of this review posits that there is a definite connection between chronic emotional stress, hyperventilation, reduced urine net acid excretion and low-grade metabolic acidosis. The author further explains that like in conventional exercises, therapeutic increase in carbon dioxide in the blood (respiratory acidosis) by way of self-induced hypoventilation is the key to eliminating metabolic acidosis.

Keywords

Chronic psychogenic hyperventilation, Hypocapnia, Low-grade metabolic acidosis, Pranayama and breath retention as an intervention, Renal compensation for respiratory alkalosis and acidosis

Introduction

A Connection between Chronic Hyperventilation and Metabolic Acidosis

Hyperventilation happens most often to people 15 to 55 years old [1]. When a person is under stress, their breathing pattern changes. Typically, an anxious person takes small, shallow breaths, using their shoulders rather than their diaphragm to move air in and out of their lungs. This style of breathing (hyperventilation) causes blood concentration of carbon dioxide (PaCO2) to fall below healthy levels, a condition called hypocapnia [2,3].

In normal subjects, a decrease in PaCO2 (hypocapnia) induced via hyperventilation, elicits two responses with opposing effects on blood pH. In the short term, a decrease in PaCO2 will increase pH of extracellular fluid (respiratory alkalosis). Over a longer period (2 to 6 hours), however, sustained hypocapnia is further compensated by renal response – renal net acid (renal ammonium and titratable acid) excretion decreases, resulting in a reduction in plasma bicarbonate and extracellular fluid acidosis [4,5]. Low-grade metabolic acidosis is a condition characterized by a slight decrease in blood pH, within the range considered normal. Chronic hyperventilation and acidogenic diets when coupled with an age-related decline in kidney function of normal humans (due to failure to excrete the normal acid load generated by protein metabolism) may further influence the occurrence of such a condition.

If these conditions occur in a prolonged, chronic way, low-grade metabolic acidosis can become significant and predispose to metabolic imbalances as well as the increased risk of chronic diseases [6].

It is conceivable that as an individual ages blood acidity will be regulated at progressively higher levels and plasma bicarbonate concentration and PaCO2 at progressively lower levels.

Such progressively worsening metabolic acidosis, however mild, might over time have deleterious effects, perhaps contributing to the pathogenesis of many of the physiologic disturbances and degenerative diseases of aging. Yet, practically no attention has been given to the effect of age on the acid-base composition of the blood in healthy subjects [7].

Breathing Retraining

The most common cause of respiratory alkalosis (hypocapnia) is hyperventilation, which causes more carbon dioxide to be exhaled out. If the cause is anxiety, various breathing exercises might help slow breathing and reduce hyperventilation [8]. There is good evidence that breathing rehabilitation is a useful method for achieving reduced anxiety/panic levels [9].

Respiratory Alkalosis (Hypocapnia) – Cause and Consequences

Respiratory alkalosis is a pathology that is secondary to hyperventilation [10]. The decrease in PaCO2 (hypocapnia) develops when a strong respiratory stimulus (i.e., anxiety or fear) causes the respiratory system to remove more carbon dioxide than is produced metabolically in the tissues [11].

Respiratory alkalosis (hypocapnia) can be acute or chronic [10]. In acute respiratory alkalosis, the PaCO2 level is below the lower limit of normal and the serum pH is alkalemic [11]. However, in chronic respiratory alkalosis, a common acid-base disturbance [5], the PaCO2 level is below the lower limit of normal, but the pH level is relatively normal or near normal due to compensatory mechanisms [11]. Since renal compensation for chronic respiratory alkalosis involves a decrease in HCO3 – reabsorption. It may also mean you have: Metabolic acidosis [12,13].

Potential Causes of Low-grade Metabolic Acidosis in Normal Subjects

Our modern lifestyle, which involves some combination of, (1) Chronic psychogenic hyperventilation as a result of the stress one experiences while sitting still and, (2) the acidogenic diet we eat, are the two main root causes for the development of low-grade metabolic acidosis.

Additionally, age-related renal functional decline in healthy older adults is another risk factor [7,14].

Pathogenesis

(1) Chronic Psychogenic Hyperventilation

Does persistent hypocapnia cause metabolic acidosis? The answer is yes! Because:

Since chronic respiratory alkalosis is a common acid–base disturbance characterized by a primary and sustained decrease in arterial carbon dioxide tension (PaCO2) – that is, by primary hypocapnia due to hyperventilation.

A decrease in PaCO2 (hypocapnia) elicits two responses with opposing effects on blood pH. In the short term, a decrease in PaCO2 alkalinizes extracellular fluid. Over a longer period (6 to 72 hours), however, renal acid excretion is inhibited, resulting in a reduction in plasma bicarbonate that acidifies extracellular fluid and thereby corrects blood pH toward normal. Furthermore, stimulation of the medullary respiratory center in metabolic acidosis induces secondary hyperventilation, resulting in a decrease in PaCO2 (secondary hypocapnia) [5].

We live in modern times, and we are exposed to the modern lifestyle – the most dangerous aspects of which are the inordinate amount of stress we experience while sitting still and the fake foods we eat [15]. It is important to note that according to research studies, over 90% of the modern population have chronic hyperventilation hidden in the modern lifestyle [16].

In normal subjects, chronic hyperventilation lowers plasma bicarbonate concentration (metabolic acidosis), primarily by inhibiting renal ammoniagenesis and the urinary excretion of net acid in response to persistent hypocapnia (PaCO2) [4,5].

(2) Acidogenic Diet

Our dietary habits tend to tip the balance towards acidification. Diets with high acid load produces changes in the acid base balance. There is an association between low-grade metabolic acidosis with the development of age-related diseases (i.e., hypertension, diabetes, chronic kidney disease etc.) [17]

Several studies have indicated the influence of low-grade metabolic acidosis on health outcomes, and diet is one of the factors that directly influence this condition [18].

(3) Age-related Renal Functional Decline

In normal adult humans eating ordinary American diets, systemic acid-base equilibrium is maintained within narrow limits from day to day, despite a continuing input of precursors of fixed acids in the diet.

Day-to-day stability of acid-base composition of the systemic circulation is critically dependent on excretion of acid in urine, the steady-state rate of which is adjusted by the healthy kidney in keeping with the prevailing rate of endogenous acid production.

Considering that renal functional integrity progressively declines with age which limit the adaptive mechanisms responsible for maintaining acid-base homeostasis, it is quite conceivable that as an individual ages, blood acidity will be regulated at progressively higher levels.

Such progressively worsening metabolic acidosis, however mild, might over time have deleterious effects, perhaps contributing to the pathogenesis of many of the physiologic disturbances and degenerative diseases of aging. Therefore, the role of age-related metabolic acidosis in the pathogenesis of the degenerative diseases of aging warrants consideration [7,14].

Chronic Metabolic Acidosis – Functional Disorders in Elderly Persons

Age-related metabolic acidosis is a chronic condition that many people in the Western world have but do not realize it [18]. Metabolic acidosis has been associated with a range of physiological derangements of importance to the health of older people such as: [19]

(i) Metabolic Alterations

Metabolic disorders of high concern in today’s society include obesity, insulin resistance and diabetes, hypoxia and oxidative stress, chronic inflammation, metabolic acidosis and metabolic syndrome, hormone imbalance, kidney disease, cardiovascular disease, excess calcium excretion and osteoporosis, and cancers of all kinds [20,21].

(ii) Organ Dysfunction

Acidosis may adversely affect renal function, cardiovascular health, muscle function and bone health, and impairments in these organ systems would be expected to have an adverse impact on physical function and quality of life – key outcomes for aged people [19].

(iii) Premature Aging and an Increased Risk of Death

Chronic tissue acidosis accelerates the ageing process and creates an environment conducive to the development of a number of diseases [22]. Stated otherwise, acidosis is the first step towards premature aging and accelerated oxidative cascades of cell wall destruction [23].

As an individual ages blood acidity will be regulated at progressively higher levels and plasma bicarbonate concentration and PaCO2 at progressively lower levels.

Such progressively worsening metabolic acidosis, however mild, might over time have deleterious effects, perhaps contributing to the pathogenesis of many of the physiologic disturbances and degenerative diseases of aging. Yet, practically no attention has been given to the effect of age on the acid-base composition of the blood in healthy subjects [7].

Solution of the Problem?

Treating age-related metabolic acidosis with lower CO2 levels is obviously a matter of raising carbon dioxide levels in the blood [24]. If your CO2 levels are too low, the first step your doctor will take is treating the underlying condition (hyperventilation) that is causing the imbalance [25]. How does one do that? The answer is already obvious. Hold your breath. Practice pranayama [15].

According to Patanjali, the founder of Yoga philosophy, pranayama is the gradual cessation of breathing. The eventual goal of pranayama is the complete suspension of the breath for as long as the practitioner wishes [26]. Holding your breath also causes the amount of carbon dioxide building up in your body [27]. Depending on your ability to tolerate CO2, you can become a super-healthy person [15]. Remember, self-induced mild hypoventilation is said to have some benefits, but of course uncontrolled or long-term hypoventilation is to be avoided [28]. It is best to maintain a normal relaxed breathing pattern whenever you are consciously aware of your breathing [29]. Fortunately, we also have the power to deliberately change our own breathing [2].

Benefits of Pranayama?

Scientific studies have shown that controlling your breath can help to manage stress and stress-related conditions [2] by normalizing the baseline levels of carbon dioxide (eucapnia) in the blood, making people less prone to hyperventilation [30].

Furthermore, practicing pranayama (breath regulation or breath control) can enhance quality of life and aid in longevity [31-33]. Other beneficial effects might involve a reduction of distress, blood pressure, and improvements in resilience, mood, and metabolic regulation [34].

In conclusion, it can be safely assumed that controlling or reversing low-grade metabolic acidosis, by yoga breathing exercises and dietary interventions, is likely to be an important way to prevent, or reduce the severity of age-related diseases.

Reversing Age-related Metabolic Acidosis – An Intervention

It is important to note that by working on the continuum of yogic breath challenge, CO2 tolerance, and renal compensation of respiratory acidosis, the goal of prevention or correction of metabolic acidosis can be achieved. Respiratory challenge is the modification of arterial carbon dioxide concentration to induce a change in cerebral function or metabolism. You can experiment with breath hold exercises in order to increase your CO2 tolerance. Although breathing is something your body naturally does, it’s also a skill that can be sharpened [35].

The yogic technique of respiratory challenge is based on a simple modification of respiratory rate, including breath hold [36]. Notably, rapid increase in Pa,CO2 evokes an immediate response by hyperventilation to restore normality. However, a more gradual increase in Pa, CO2 allows renal compensation to occur [37].

Improved CO2 tolerance reduces the urge to breathe and controlled breath holding (hypoventilation) will build CO2 endurance [28]. Research has shown that lower chemosensitivity to hypercapnia in yoga practitioners may be due to an adaptation to low arterial pH and high PaCO2 for long periods [38,39].

Because renal compensation of respiratory acidosis occurs by increased urinary excretion of acid (hydrogen ions) and resorption of HCO3 [40]. By adjusting the amounts of hydrogen ions secreted in the urine and reabsorption of bicarbonate ions from the urine back to the blood, kidneys balance the blood pH, and [41] as a result, in a compensated respiratory acidosis, although the PCO2 is high, the pH is within normal range [42], [43-45].

Since, breathing is an automatic function of the body that is controlled by the respiratory centre of the brain. When we feel stressed, our breathing rate and pattern changes as part of the ‘fight-or-flight response’. Fortunately, we also have the power to deliberately change our own breathing [2]. Hence changing your breathing to a normal pattern can restore healthy CO2, which will eliminate symptoms of hyperventilation [29]. Further, by adjusting the speed and depth of breathing, the brain and lungs are able to regulate the blood pH minute by minute [46,47].

Biomedical Importance of Carbon Dioxide

CO2 plays various roles in the human body including regulation of blood pH, respiratory drive, and affinity of hemoglobin for oxygen (O2). Fluctuations in CO2 levels are highly regulated and can cause disturbances in the human body if normal levels are not maintained, [48] causing disruption of enzyme function, loss of insulin sensitivity, and cellular metabolic adaptations [49].

Benefits of Carbon Dioxide

Carbon Dioxide Dependent Signal Transduction

Research shows that changes in CO2 influence cellular function through modulation of signal transduction networks. Additionally, there is significant cross-talk between these signal transduction pathways as they respond to changes in CO2 [50-52].

Elevated CO2 regulates the Wnt signaling pathway in mammals [53]. The Wnt signaling pathway is one pathway that may contribute to aging [54]. Downregulation of Wnt signaling is an early signal for formation of facultative heterochromatin and onset of senescence in primary human cells [55].

Natural Sedative and Tranquilizer

CO2 is a powerful natural sedative and tranquilizer [56]. Carbon dioxide calms the nervous system, reducing depression, anxiety, and even the symptoms of epilepsy [57].

While considering the mental health of people, the most missing chemical in the human brain is CO2 [29]. Because a lack of CO2 in the brain leads to “spontaneous and asynchronous firing of neurons” (medical quote) “inviting” virtually all mental and psychological abnormalities.

In conscious humans, research findings suggest that increased CO2 levels (mild hypercapnia) cause a reduction in the resting-state neural activity of the brain, which in turn, enters a lower arousal state [58].

Bioavailability of Oxygen

Carbon dioxide is essential for internal respiration in a human body [59]. Increase in the blood concentration of carbon dioxide lowers hemoglobin’s affinity for oxygen, which in turn, enhances the unloading of oxygen into tissues to meet the oxygen demand of the tissue. The Bohr effect describes hemoglobin’s lower affinity for oxygen secondary to increases in the partial pressure of carbon dioxide and/or decreased blood pH [60].

Gardian of the pH

Carbon dioxide is a guardian of the pH of the blood, which is essential for survival. The buffer system in which carbon dioxide plays an important role is called the bicarbonate buffer system. It is an acid-base homeostatic mechanism in order to maintain pH in the blood, among other tissues, to support proper metabolic function [59,61].

Anaplerosis

CO2 is a ubiquitous product of cellular metabolism and an essential substrate for carboxylation reactions, required for refueling the TCA cycle via oxaloacetate during growth on glycolytic carbon (pyruvate) sources. Remember, De novo protein synthesis is required for adaptive response to oxidative and other types of stress, indicating that newly synthesized protective proteins are necessary for adaptation [62].

Cardiovascular System

CO2 is helpful in dilating the smooth muscle tissues, and it regulates the cardiovascular system [63].

Breathing

Breathing is exquisitely sensitive to the alteration in the CO2 tension of the blood [64]. Regularity and Smoothness of Breathing are controlled by CO2 [56].

Bicarbonate (Electrolytes) in Cell Volume Regulation

Bicarbonate (a form of CO2) belongs to a group of electrolytes, which help keep your body hydrated and make sure your blood has the right amount of acidity [65]. Compared to the widely studied roles of sodium, potassium, and chloride in cell volume regulation, the effects of proton and bicarbonate are less understood [66].

Bicarbonate Dependent Soluble Adenylyl Cyclase Activity

As Soluble adenylyl cyclase (sAC) activity is stimulated by HCO3(-). In the absence of sAC, lysosomes fail to fully acidify, lysosomal degradative capacity is diminished, autophagolysosomes accumulate [67].

Numerous Other Benefits of CO2

Fat-burning

Carbon dioxide increases fat burning through the peroxisomes. When the carbon dioxide levels are low, there is less activity in the peroxisomes [68].

Antioxidant

Carbon dioxide is an antioxidant and prevents oxidative stress. CO2 interacts both with reactive nitrogen species and reactive oxygen species. Several mechanisms have been suggested to explain the protective role of CO2 in vivo [69].

Anti-inflammatory Effects

CO2 reduces inflammation throughout the entire body [15]. Recent advances have identified the repression of the NF-κB transcriptional pathway by CO2 in a manner which may be of therapeutic benefit in chronic inflammatory disease [70].

Antimicrobial Activity

Carbon dioxide gas is an antiviral, antibacterial, and anti-infection agent effective not only on solid surfaces but also in aqueous solutions and water treatment settings [71].

Bone Mineralization

Ancient yogis enjoyed a reputation for having incredibly strong, “unbreakable” bones.

Decongestant

It relieves nose and sinus congestion.

Improved Digestion

CO2 stimulates hydrochloric acid production in the stomach.

Cancer Prevention

Carbon dioxide helps oxygen to pass into cells. When cells are properly oxygenated, they are more likely to burn energy efficiently.

Healthy Skin

It helps the skin maintain itself, giving you a soft, buoyant glow, as opposed to the sunken, dry pallor of a stressed-out asthma sufferer.

Carbon Dioxide Structures Water

It is the body’s ability to structure water when it has a healthy dose of carbon dioxide to combine with oxygen and electrolytes. The body becomes a living battery filled with an electrical charge! This is the true magic behind holding the breath. Structured water also acts like a liquid crystal, and it conducts electricity like a superconductor.

Performance

CO2 increases blood flow. There is a relationship between blood flow volume and muscle fatigue, and increased blood flow reduced muscle fatigue. Second, Overtime, increased CO2 stimulates the mitochondria in your cells to multiply. The more mitochondria you have, the more energy you have increasing your level of performance in everything you do [15].

Solvent Power

Forming an acid as it dissolves in water, carbon dioxide increases the solvent powers of many substances. The oxygen in the atmosphere becomes available to living cells as it is dissolved in the liquid medium outside and inside the cell [72,73].

Plasma Membrane Permeability

CO2 not only freely diffuses through the cellular membrane, it may also accumulate in the same, thus increasing its permeability and fluidity [74]. Cell membrane plays a very important role in the maintenance of cellular homeostasis. On the one hand, the membrane prohibits the entry of toxic or unwanted substances. On the other hand, it also prevents the exit of important or useful substances [75].

Gene Expression

Carbon dioxide (CO2) is sensed by cells and can trigger signals to modify gene expression in different tissues leading to changes in organismal functions [53]. While the effects of in vivo hypercapnia on gene expression are likely to occur in part through indirect mechanisms such as altered neuronal activity or the release of stress hormones, recent evidence suggests that CO2 may also directly regulate gene expression through the NF-κB pathway [76].

Vagus Nerve Stimulation

Carbon dioxide produces a vagotropic effect. Since carbon dioxide has a direct effect on the nuclei of vagus nerves and exerts a stimulating effect [77]. Furthermore, cardiac slowing by hypercapnia occurs through a direct effect of CO2 rather than pH and that the mechanism has both central and peripheral mediation; the former transmitted by vagal pathway with a specific site of action at the sinus node [78].

Increasing your vagal tone activates the parasympathetic nervous system, and having higher vagal tone means that your body can relax faster after stress to overcome anxiety and depression, and better manage them when they arise.

Studies have revealed that slow respiration and extended exhalation stimulate vagus nerve. This results in parasympathetic nervous system (PNS) over sympathetic nervous system (SNS) dominance, structural and functional changes in higher cortical areas through autonomic projections, and is thus responsible for effects on physical health, mental health and cognition [79].

Summary

When the body becomes more acidic with age blood carbon dioxide concentration will be regulated at increasingly lower levels [7].

A lack of carbon dioxide is itself a starting point for different disturbances in the body. If carbon dioxide deficiency continues for a long time then it can be responsible for diseases, ageing and even cancer [80].

Internal acid–base homeostasis is fundamental for maintaining life. Normal acid-base homeostasis requires that both CO2 and HCO3(a form of CO2) be normal [81].

Continuously produced through respiration – we produce about 1.0 kg of CO2 per day – it forms the bicarbonate/CO2 pair, our main physiological buffer [82].

The carbon dioxide–bicarbonate system is important in maintaining homeostatic control, which regulates an organism’s internal environment and maintains a stable, constant condition of properties like temperature, pH, heart rate, blood pressure, satiety (fullness), and circadian rhythms (sleep and wake cycles) [83].

Important

Chronically low CO2 levels may be associated with health risks:

When the blood is acidic and HCO3- levels are low, the body’s natural response is to increase its breathing rate. By breathing faster, more CO2 is exhaled out of the body, which decreases CO2 blood levels [25].

A lack of carbon dioxide is itself a starting point for different disturbances in the body. If carbon dioxide deficiency continues for a long time then it can be responsible for diseases, ageing and even cancer [80].

Treating age-related metabolic acidosis with lower CO2 levels is obviously a matter of raising carbon dioxide levels in the blood [50].

In conclusion, Carbon dioxide (CO2) is a fundamental physiological gas [24]. Therefore, CO2 homeostasis is indispensable for life [84]. “The theory of life, in brief, is such that carbon dioxide is the basic nutrition of every life form on Earth…. It acts as the regulator of all functions in the organism, it maintains the internal environment of the organism, it is the vitamin of all vitamins.” Dr. K. P. Buteyko [85].

Yale University applied physiology professor Yandell Henderson considered carbon dioxide as a “chief hormone of the entire body; it is the only one that is produced by every tissue and that probably acts on every organ.”

From naturopathic doctors (ND’s) perspectives, carbon dioxide can indeed be seen as the missing link in dealing with stress. They have explored how depleted carbon dioxide, or hypocapnia, can have a role in altering pH levels, disrupting oxygen delivery to the tissues, and perpetuating stress [86].

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FIG 1

Does Recognition of Emotions Differ in People with and without Chronic Pain when a Lower Face Covering is Used? A Cross Sectional Study

DOI: 10.31038/PSYJ.2023513

Abstract

Background: As a result of the Covid panidemic, face masks are routinely used around the world. These could hamper the recognition of (basic) emotions and lead to misunderstandings. People with chronic pain typically struggle with (non-) verbal communication, and their behavior is frequently interpreted differently by their surroundings. This paper examines the variance in emotion recognition (accuracy and speed) between individuals with chronic pain and asymptomatic subjects.

Methods: Four validated measures (Central Sensitization Inventory (CSI), Graded Chronic Pain Scale (GCPS), The Brief Pain Inventory (BPI, and the Toronto Alexithymia Scale (TAS-20)) were used to differentiate between the asymptomatic control (CP) and chronic pain group (PG). In addition, a computerized emotion recognition test (ERT) comprised of 42 morphed images depicting six fundamental emotions (with) out lower fase covering was utilized to measure the accuracy and time required for this study.

Results: The recruitment and analysis of 170 patients included 98 subjects with chronic pain (PG). There was a statistically significant difference (p<0.01) between CP and PG (with) out lower face covering in the recognition of all basic emotions. In both groups, fear (from 57.1% to 66.3%) and disgust (from 67.1% to 69.1%) were more prevalent in the PG. Sadness and disgust (p<0.0001) in the PG were especially harder to identify when the lower face was concealed. There was no statistically significant difference in time between CG and PG without facial covering (p=0.34, p=0.4).

Conclusion: People in a chronic pain state have more difficulty with emotion recognition without lower face covering, but there is no difference in time. Emotion recognition, especially sadness and disgust with face covering are significantly diminished in PG were disgust was frequently confused with anger and fear with astonishment.

Significance: As indicated in this paper, distributing collected data of perceived emotions supplied by a standardized ERT and perceived emotions by the participants in a table (confusion matrix) may provide a clear overview of correct and incorrect answers, both within and between the two groups.

Introduction

When facial emotion recognition is present, a reflexive ocular scan of the face occurs, allowing the emotion to be interpreted by detecting the underlying muscles involved [1,2]. In this way, observers attempt to direct their attention to critical components to distinguish facial emotions [3]. According to Guo [4], the eye region, as well as the nose and the mouth are frequently observed to differentiate emotional expressions. Other studies indicate that fundamental emotion expressions are associated with a set of features expressed in our face; for example, fear is associated with gestures at the eye levelprimary facial feature change to help facilitate the identification of this emotion. In comparison, joy would be primarily associated with gestures/movement of the lips in order to facilitate its recognition, but the region of the brows, cheeks, and lower eyelid tension all contribute to its detection [5]. The lower face appears to have been strongly associated with feelings of satisfaction and attractiveness [6]. For instance, it is stated that facial regions such as the nasolabial fold and commissure broad, as well as the upper lip vermilion, are critical for identifying mood levels like satisfaction [7]. According to some studies, the left side of the face exhibits pleasant but contrived facial expressions, whereas the right half exhibits genuine feelings [8]. The lower half of the face conveys pleasant and joyful emotions, while the upper half conveys surprised and anxious emotions. On the upper-lower face axis, spontaneous facial expressions are more prevalent than on the left-right facial axis. This may account for everyone’s unique search pattern for emotion identification, which is not predictable but varies according to task, face location, and face coverage [9].

The Covid19 epidemic has facilitated the widespread usage of facemasks around the world. While facemasks aid with infection prevention, there are worries about their influence on facial recognition, expression, and hence social communication [10]. According to the most widely accepted theory of face perception, emotional expression recognition and facial identity are distinct perceptual processes encoded by distinct psychological [11] and neural mechanisms [12,13]. More precisely, in terms of emotion identification and expression, multiple studies have examined the quantity and kind of social information provided by various parts of the face, concluding that the mouth plays a critical role in understanding emotions, particularly basic emotions like happiness and disgust [14,15]. Indeed, the mouth is a vital component of human face recognition, being almost symmetrical and typically visible from any angle, making it the ideal aspect to focus on in all those instances where the user can be scrutinized from any angle. According to a recent study, mouth-based emotion identification does not vary from full-face emotion recognition but greatly supports subtle emotion recognition in general [16]. As a result, it is obvious that covering the lower half of the face with a facemask impairs emotion perception. While the absence of facial processing signals can be compensated for by more expressive gestures, and cognitive and coping strategies, covering the lower half of the face with a facemask may have resulted in higher disability in individuals with impaired compensation abilities, such as deafness, congenital prosopagnosia, and autism.

Little is known about how individuals with chronic pain conditions cope with emotion recognition during face mask-wearing. Chronic pain is defined as any type of pain that persists for more than three months, either continuously or recurrently [17,18]. It is estimated to affect 20% of the population and imposes a massive cost on both individuals and the healthcare system (Goldberg et al. 2011). Current models of chronic pain demonstrate the complex interaction of sensory, environmental, psychological, and pain regulation risk variables that contribute to an individual’s pain vulnerability [19] and may lead to chronic pain maintenance (Koechlin et al. 2017). Chronic pain may also be associated with Alexithymia which may be one of the characteristics of emotional dysregulation in chronic pain [20] and is characterized by difficulties identifying (i) and describing emotions (ii), as well as externally oriented thinking (iii) [21]. It is identified in a variety of chronic pain conditions, including Low back pain (LBP), chronic facial pain and Temporomandibular Disorders (TMD), fibromyalgia, chronic migraine, irritable bowel syndrome and Complex Region Pain Syndrom (CRPS) [22] among others. Alexithymia is often associated with depression or depression feelings (Saariaho et al. 2017). It is hypothesized that long-term peripheral nociception alters brainstem and central nervous system circuits, resulting in the spread of pain and perceptual abnormalities of the body schema [23]. This somatorepresentation distortion may result in a perturbation of the somatosensory-motor system [24] and is strongly associated with the inability to recognize refined (facial) motor patterns. For instance, in experimental research involving chronic low back pain (CLBP) [25] TMD, and chronic facial pain, the accuracy and speed of basic emotion identification are decreased, and other asymmetrical performed emotions such as disgust and fear are exchanged by others [26]. Based on this data it is an obvious question whether wearing a surgical mask or covering the lower face has an impact on the accuracy and time of recognition of the (basic) emotions in persons in a chronic pain state more than people without pain.

On the basis of this information, the primary aim of our study is to examine whether emotion recognition (accuracy and time) is different between asymptomatic subjects and those with chronic pain when the lower face is covered as when wearing a surgical mask. We hypothesized that persons with chronic pain would perform worse at emotion recognition in all conditions when compared with asymptomatic subjects.

Material and Method

Participants and Sample Size

A sample size of 160 participants was calculated a priori via power analysis [27] targeting a repeated measures analysis of variance (ANOVA) with six groups (emotions) and four measurements (mask vs. no mask in control and pain group) and the ability to detect a medium effect size of f=0.25, given an α=0.05 and a test power (1-β)=0.80. Since the actual number of participants recruited was greater than the required number, a post hoc power calculation demonstrated that a power of 0.88 was reached.

Inclusion criteria were participants between 18 and 60 years, able to understand the task and to recognize and click on the specified emotions on a laptop with a computer mouse. Individuals were excluded if they could not write or speak German, as well as those who had impairment in the hand or vision.

Measuring Instruments

The measurements were divided into two sections. The first section requested demographic information as well as five questionnaires regarding participants’ current health status. The second section was a computer task that consists of two sets of 42 pictures depicting basic emotions, with (out) covering the lower face.

Questionnaires

Central Sensitization Inventory (CSI)

The CSI is a screening instrument to help identify symptoms related to central sensitization or indicate the presence of a central sensitive dysfunction [28]. In this study, the entire sum of part A (central sensitisation characteristics) is computed. Part B is not utilized; it is just for the purpose of providing information on existing diagnoses in the domain of central sensitisation. It is comprised of 25 multiple-choice questions with a possible score of zero to four (never – rarely – occasionally – often – always). As a result, a possible total score of 100 is possible. A score of 40 or more points has been reported to be indicative of central sensitisation.

Graded Chronic Pain Scale (GCPS) is a validated standard self-assessment tool used in clinical pain research and quality management. It provides a hierarchical classification system (I-IV). The outcomes are classified into four subgroups, with grades I and II seen as a slight limitation (functional chronic pain) and grades III and IV as strong limitations (dysfunctional chronic pain) [29]. In our study we used the German validated version and included volunteers who are classified as grade II, III and IV [30].

The Brief Pain Inventory (BPI) was established to give a quick and simple method for determining the level of pain and the extent to which pain interferes with lives of patients with pain [31]. Four questions concerning pain intensity and seven about pain interference are asked, as well as four about the present pain experience, the region of discomfort, the medicine or therapy used to alleviate the pain, and the extent of treatment outcome. On a scale of 0 to 10, pain intensity (no pain to the most severe agony imaginable) and pain interference (no interference to complete interference) are quantified. The responses to the questions on pain severity are put together and divided by four. After summarizing the responses about the impact of pain, they are split by seven. As a result, a total of 11 points may be earned. If a responder scores more than five points or answers more than four questions with pain, the test is deemed positive for pain-related disability. We used the validated German language BPI in our investigation.

Beck Depression Inventory (BDI). The BDI consists of twenty-one items that assess the frequency and severity of depression symptoms. The maximum score is 93 points (severe depression) and a mild depression can be observed from 14 points. The responses are computed on a zero-to-three-point scale and demonstrate a high reliability of 0.92 (Chronbach’s alpha) and acceptable validity of 0.73 to 0.96 for discriminating between depressed and non-depressed participants.

Toronto Alexithymia Scale (TAS-20). A subjective self-evaluation questionnaire which allows making a reliable identification alexithymia characteristics (i.e., difficulty in identifying emotions, difficulties in describing emotions, and externally oriented thinking style) [32,33]. The items of this tool are graded on a 5-point Likert scale, with answers ranging from “strongly agree” to “strongly disagree.” The points are totaled up to a maximum of 100 points. Scores under 51 on this scale indicate no alexithymia. A score of 51-60 points indicates a transitional period in which alexithymia may be present. Scores of 61 or more indicate alexithymia [34].

Emotion Recognition Task (ERT)

The CRAFTA Facial Recognition Test and Training software was used to perform this task ( www.myfacetraining.com) and was divided in two sections. The first part was the recognition of basic emotions (happiness, sadness, disgust, anger, fear, and surprise) without lower face covering. Participants were required to choose the appropriate expression for each image shown by clicking on it with their mouse on the screen of the PC (maximum time to choose was 5 sec.). Accuracy and time to respond for a standardized sequence of 42 pics were measured. After this first task, the participant had one minute rest followed by the same standard test but the pictures had a lower face covering (i.e., lower face mask)(section 2) (Figure 1).

FIG 1

Figure 1: Emotion Recognition Computer task (ERT). Example of a morph from neutral face (1a) expressed in the basic emotion (in this case disgust) with (out) lower face covering in a shape of a mask (1b and 1c). (1d) are the choices the participant can click on. Computer program calculates the time and the (in) correct choices of 42 pictures.

Procedure

The study was run from November 2020 until June 2021during the COVID-19 pandemic when general legal obligations to wear masks in Germany were already in action. Volunteers were recruited randomly in the mid-west of Germany through physiotherapy clinics, (sports) organizations, local universities, and via posters and advertisements. The procedure was done by 3 assessors (physical therapists) with more than 5-year of experience. The assessors were calibrated by specific training. Prior to the experimental session, written informed consent was obtained from each participant. All data were collected anonymously. Firstly, the participants were informed about the aim of the study and then were asked to sign a consent form, fill the questionnaires followed by the emotion recognition task (ERT). Before starting the ERT, a one-minute explanation and a trial with 5 random images was obligatory. Afterwards the ERT with (out) lower face covering was executed. A fourth (blinded) assessor anonymously acquired the data and classified the participants into groups such as control (CG) and (chronic) pain (PG) and carried out data analysis.

Statistical Analysis

Data was collected into SPSS 26 and the data of emotion perception of the CG and the PG (with) out lower face covering are distributed in a confusion matrix. The the Chi² test was used calculating statistical significance. Pearson Chi-square test was used to determine differences between conditions using nominal data (e.g., gender, age. BMI.). Mann-Whitney/U-Test for ordinal and metric data were performed for continuous data (e.g., age, BMI, questionnaires, time to respond), as the cohorts were not normally distributed. The significance level was set at 5%.

Results

Participants

In total 170 subjects were recruited and analyzed. From these, as mentioned two groups were created. The control group (CG), as mentioned above did not refer pain based on their responses to the questionnaires. The second group includes all subjects who were regarded as having chronic pain (PG), based on their answers to the questionnaire’s medication usage was not asked. Table 1 summarizes the demographic data and questionnaires scores for both groups.

Table 1: Demographic data and mean scores of questionnaires by group (control group (CG) and chronic pain group (PG)). Y=years, M=Months F=Female.

TABLE 1

 

Based on the information from the questionnaires, participants were divided in two groups:

Asymptomatic Subjects (control group). Those subjects who based on their answers were identified as not having pain (CSI<40 p., GCPS class I, BPI score less than 5 points and less than 4 questions with pain, TAS-20<51 p., BDI<14 p.)

Subjects with chronic pain (pain group). Those subjects who reported pain as evidenced in their responses to all questionnaires (CSI >40 p., GCPS class 2-4, BPI score 5 points and more than four questions with pain, TAS-20 >51, BDI>14 p.) and whose pain was longer than 3 months.

Accuracy of Emotion Recognition and Confusion

By this analysis we wanted to explore whether subjects with chronic pain were less accurate than asymptomatic subjects at distinguishing basic emotions and were confused with the emotion recognition. We examined the data in a modified confusion matrix recently described by Carbon et al. (Table 2). Hereby the emotions displayed by the program (perceived) are compared to the emotions specified by the test participants. (recognized) The answers highlighted in orange are the participants’ greatest percentages, the red ones indicate the most frequent confusion about an emotion and the green boxes the most wrong chosen emotion.

Table 2: Confusion matrix of expressed and perceived emotions of control group (CG) and pain group (PG) with (out) mask. Segments of the table in orange are the highest score. The red segments indicate the most frequent confusion about an emotion.

TAB 2(1)

TAB 2(2)

 

Without Lower Face Covering. In both groups, happiness was recognized the most (CG: 92.5%, PG: 91%) and it was exchanged dominantly with fear (CG: 1.6%, PG: 1.9%). Fear was recognized the least in both groups (CG: 30.6%, PG: 28.1%) and was most often mistaken for astonished (CG 37.8%, PG 36.4%). Fear also had the highest number of incorrect answers (CG 57.1%, PG 58.2%; highlighted in green in Table 2).

With Lower Face Covering. Happiness was best recognized in both groups (CG 90.7%, PG 87%). A small number of participants in the CG mixed up happiness with anger (1.6%) and astonished in the PG (1.9%). More strikingly, disgust was recognized much less in both groups (CG 28.4%, PG 26.1%) and was mostly confused with anger (CG 40.9%, PG 40.7%).

Incorrect Chosen Emotion. Based on our results, it seems that with (out) covered lower face “fear “is the most chosen incorrect emotion (without; CG=57.1%, PG 58.2% and with: CG=68.4%, PG 66.3%). A clear misjudgment was also made in both groups of “disgust“ with lower face covering (CG 67.2%, PG 69.1%) but less without lower face covering (30.5%, PG 30.4%). In Table 2 they are highlighted in green.

Confused Emotions. In both groups more than 40% confused “disgust” with “anger” (CG 40.9%, PG 40.7%) when the lower face was covered which was clearly more than without covering (CG 14.9%, PG 13.8%). Also “fear” clearly swopped with “astonishes\d” in both groups without (CG 37.3%, PG 36.4%) and with lower face covering (CG 23.8%, PG 25.2%)

Differences with (out) Face Covering in Both Groups and Answering Time

An overview of the mean percentage of correct basic emotions with (out) covered lower face (red) of control group (CG) and the chronic pain group (PG) are depicted in Figures 2 and 3. It may be concluded that between the CG and the PG with (out) face covering showed a clear significant (P<0.001) difference of all basic emotions except that of happiness. In the PG there is an extremely significant difference in emotion recognition with (out) lower face covering times of “sadness” and “disgust” (Figure 3) Average answer time. The average time of the CG (with) out face covering was both 3,1 (±0.8), p=0.2 and the PG 3,2 (±0.9), p=0,3. There was no significance in time between CG and PG with (out) face covering (p=0.34 p=0.4).

FIG 2

Figure 2: Mean percentage of correct basic emotions with (out) covered lower face (red) of the control group (CG) (n=72) Asterisks indicate statistical differences between conditions of wearing and non-wearing on basis of paired t-tests: *p < 0.05, **p < 0.01, ****p < 0.0001; ns, not significant.

FIG 3

Figure 3: Mean percentage of correct basic emotions with (out) covered lower face (red) of the chronic pain group (PG) (n=98) Asterisks indicate statistical differences between conditions of wearing and non-wearing on basis of paired t-tests: *p < 0.05, **p < 0.01, ****p < 0.0001.

Discussion

In the present study we tested the impact of covered lower face with imitated face masks on basic emotion recognition during a computer task. We confronted participants with (out) chronic pain with faces in a neutral emotion that morphed into one of six different basic emotions (angry, disgusted, fearful, happy, astonished and sad) during a computer standard test. Variables we tested were accuracy and time. Control group (CG); comparing the results of the CG with the results of Carbon (2020) it should be noted that they do not completely match. Without a mask, the Carbon study had a 92.5% recognition for the fear emotion and while only 30.6% did in this study. Happy (Carbon 98.8% to 92.5%) and angry (Carbon 83.7% to 74.2%) were also recognized significantly differently. With mask, happy is recognized 90.7% correctly in this work and only 74.2% during the study of Carbon. At Carbon, fear is still correctly recognized 93.5% of the time when wearing a face mask, but only 29.8% in our study. Even sadness is recognized correctly with 62.6% during the Carbon study and in ours 41.7%. Disgust and angry are almost the same in the CG. It may be concluded that the same trend of accuracy in both studies of the different emotions can be observed, but not of all outcomes.

In both groups it can be registered that sadness and disgust are significantly less recognizable with a covered lower face. A feature of these two emotions is that they have excessive asymmetric facial expression changes in the lower face [35]. In the Carbon study ‘astonished” was not tested and in our study astonished was also significant in both groups (with) out lower face covering. Happiness was with (out) face covering in our study was not significant in both groups (CG p=0.053, PG p=0.041) in contrast to the Carbon study.

The possible differences in results may be determined by the difference in test set-up. Carbon’s study only used photos of the basic emotions without seeing the neutral state of different ages and gender with (out) an artificial mask and there was no time limit. In our study we used a computer program with morphing of basic emotions with (out) a covered lower face with a double task; emotion recognition and recognition as soon as possible (time). In our study the neutral state of the recognized person is not measured.

Controle Group (CG) versus Pain Group (PG); the results of CG and PG, indicates that accuracy in emotion recognition was strongly reduced in both groups with (out) lower face covering. This seems to be compatible with parts of the literature employing different types of covering, for instance, by rigidly covering the mouth area with cardboard, using the bubbles technique or, much closer to the present study, using block based partial square face covering [36] or a shawl or cap. For fearful faces, as shown before in the literature, the upper face, special the eye region, which was not covered, was most relevant for judging someone emotional state. It is evident that in our sample with chronic pain there is an obvious significant difference in nearly all emotions with control group except happiness (Figure 2), but an obvious significant difference with without lower face covering (Figure 3). This observation suggests that chronic pain patients with, for example, a surgical mask have facial emotional communication impairments in emotional perception, expression special in the emotions “sadness” and “disgust” [37-43].

Strength and Limitations

As far the authors concerned, with respect to the literature this is the first observational study on face recognition with covering the lower face done in patients with a chronic pain state. The strength is that the PG sample is a clear primary or secondary chronic pain group diagnosed by questionnaires as suggested by the International Association of Study of Pain (IASP) [37]. Therefore use of medication and the medical diagnosis is not asked. This may influence the variability of the results within the PG for example in the speed of recognition. The authors are aware of this, but concerning the literature on chronic pain it is more seen as a disease in itself, we left out the individual pain regions, medical diagnosis and medication usage. A limitation may be that the covering of the lower face was not a real mask but a substitute of plastic material that has the form of a mask. This may influence the results of the variables accuracy and time. On the other hand, the test was conducted in both groups in the same way.

Conclusion

  • People in a chronic pain state are worse at emotion recognition (with) out lower face covering than persons without pain but there is no difference in time
  • Recognition of all basic emotions especially sadness and disgust with lower face covering are clearly reduced in the chronic pain state group and disgust was often confused with anger and Fear with astonished.
  • This results support the need of face rehabilitation and training which may contribute appropriate non-verbal communication and quality of life in persons with chronic pain
  • Future studies in subclassification of chronic pain (use of medication, risk factors) and outcome studies in facial emotion training may support the influence of face covering like wearing a mask.

Acknowledgements

We thanks Jetske Olde Oudhof and Karin Jungmann for their assistance in participant recruitment and assessment and Dr. Jennifer Nelsson for prove reading.

Conflict of Interest

There is no conflict of interest

Authors’ Contributions

HP conceived the design, AG was in charge of data collection, and all authors participated in data analysis and discussed the findings. HP composed the initial draft of the manuscript. All authors contributed to the final version of the paper.

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Proficiency Monitoring of Allergen-Specific IgE macELISA – 2022

DOI: 10.31038/MIP.2022333

Abstract

The purpose of this study was to evaluate the reproducibility of results yielded using a macELISA for detection of llergen specific IgE in dogs and cats when run by ten different individuals in seven separate affiliated laboratories. Samples of 24 different sera samples were independently evaluated in each laboratory by differing operators in a single blinded fashion. For evaluations completed by multiple operators in a single laboratory, the average inter-operator variance was calculated to be 7.9% (range=6.5%-9.0%). The average intra-assay variance among reactive assay calibrators in all laboratories was 7.3% (range=2.8%-17.6%). The overall inter-assay inter-laboratory variance evident with reactive calibrators was consistent among laboratories and averaged 15.7% (range 14.8%-16.7%). All laboratories yielded similar profiles and magnitudes of responses for replicate unknown samples; dose response profiles observed in each of the laboratories were indistinguishable. Correlation of EAU observed for individual allergens between and among all laboratories was strong (r>0.85, p<0.001). Collectively, the results demonstrated that ELISA for measuring allergen specific IgE is reproducible, and documents that consistency of results can be achieved not only in an individual laboratory, but among different operators and between laboratories using the same ELISA.

Keywords

IgE, ELISA, Proficiency, Atopy, Allergy, Immunotherapy, Cross-reactive Carbohydrate

Introduction

Stallergenes Greer maintains a proficiency monitoring program for laboratories that routinely run macELISA [1] for evaluation of allergen specific IgE in serum samples. The foundation for this program is based on the desire for inter-laboratory standardization and quality control measures that ensure the uniformity, consistency, and reproducibility  of results among laboratories  that  perform the assays. This program is designed to evaluate the proficiency of laboratories and  ensures  that individual operators yield consistent and  reproducible  results.  The inaugural proficiency evaluations, initiated in 2009 and repeated in 2010, in six different laboratories documents that inter-laboratory standardization and quality control measures in the veterinary arena are on the  immediate  forefront  and  that  uniformity,  consistency,  and reproducibility of results between laboratories is achievable [2]. Similarly, reproducibility of results among different laboratories was documented in the subsequent proficiency evaluations completed in 2013 [3], 2016 [4], 2018 [5], 2019 [6], 2020 [7], and 2021 [8]. The results presented herein summarize the comparative results observed in the affiliate laboratories for the most recent proficiency evaluations that were completed in 2022. The 2022 proficiency evaluation is the fourth documentation of the assay reproducibility since adopting a cross- reactive carbohydrate inhibitor in the sample diluent [6-9].

Materials and Methods

All serum samples, buffers, coated wells, calibrator solutions, and other assay components were aliquants of the respective lots of materials manufactured at Stallergenes Greer’s production facilities (located in Lenoir, NC, USA) and supplied as complete kits to the participating laboratories along with the exact instructions for completing the evaluations.

Participating Laboratories

Seven independent Veterinary Reference Laboratories (VRLs) participated in the 2022 proficiency evaluation of macELISA. Participating laboratories included three separate IDEXX laboratories located in Memphis, Tennessee, Kornwestheim, Germany, and Markham, Ontario Canada. Other affiliated European laboratories that participated in this evaluation included Agrolabo (Scarmagno, Italy), Laboratories LETI Pharma (Barcelona, Spain), and Ceva Biovac (Beaucouzé, France). Stallergenes Greer Laboratories (Lenoir, NC) served as the prototype for evaluation of the macELISA; the 2022 evaluations included results reported by four separate and independent operators. Because the performance characteristics of macELISA in Stallergenes Greer’s VRL have been well documented for use over an extended period [1-8], all results observed in the other participating laboratories were compared directly with the results observed in Stallergenes Greer’s reference laboratory.

Serum Samples

Separate pollen and mite reactive serum pools or non-reactive sera pools were prepared from cat and dog serum samples that previously had been evaluated using the macELISA for detection of allergen specific IgE. The reactivity of each sera pool ranged from nonreactive to reactive for multiple pollen or mite allergens. These sera pools and admixtures of the pools were used to construct a specific group of samples that exhibited varying reactivity to the allergens included in the evaluation panel. Twenty-four samples were included in the blinded evaluation conducted by each laboratory. Identical replicates of the high, low, and negative controls routinely used in the assay were also included as unknown samples. Also included in the array of samples was a five tube three-fold serial dilution of a highly pollen reactive pool, diluted into non-reactive sera, which served to document the dose response evident within the assay. All samples were stored at -20 °C for the interim between testing.

Calibrators

Mite reactive calibrator solutions of predetermined reactivity in the macELISA were prepared as three-fold serial dilutions of a sera pool highly reactive to Dermatophagoides farinae, Acarus siro, and Tyrophagus putrescentiae. Replicates of each were evaluated in each assay run and served as a standard response curve for normalizing results observed with the various samples. All results were expressed as ELISA Absorbance Units (EAU) which are background-corrected observed responses expressed as milli absorbance.

Buffers

The buffers used throughout have been previously described [1- 8], and included: a) well coating buffer: 0.05 M sodium carbonate bicarbonate buffer, pH 9.6; b) wash buffer: phosphate buffered saline (PBS), pH 7.4, containing 0.05% Tween 20, and 0.05% sodium azide; c) reagent diluent buffer: PBS, pH 7.4, containing 1% fish gelatin, 0.05% Tween 20 and 05% sodium azide. The buffer used for dilution of serum samples was identical to the reagent diluent buffer, but it has been supplemented (2.5 mG/mL) with a cross-reactive carbohydrate inhibitor derived from the carbohydrate components present in bromelain (BROM-CCD) [9]. BROM-CCD was prepared in house and remains a proprietary product of Stallergenes Greer (Lenoir, NC, USA).

Allergen Panel

The allergen panel was a 24 allergen composite derived from the array of allergens that are included in the specific panels routinely evaluated in the various laboratories; the composite allergen panel consisted of 4 grasses, 6 weeds, 6 trees, 5 mites, and 3 fungi. The protocol for coating and storage of wells has been previously described [1-8]. Immulon 4HBH flat bottom 12 well strip assemblies (Thermo Electron Corporation, Waltham, MA) were used throughout and served as the solid phase for all assays. The individual extracts were diluted in bicarbonate buffer (pH 9.6) and 100 µL was added to each assigned well. Following overnight incubation at 4-8°C, the wells were washed with PBS, blocked with 1% monoethanolamine (pH 7.5) then air dried and stored at 4-8°C in Ziploc bags until used.

Sample Evaluations – macELISA

The operational characteristics and procedures for the macELISAs have been previously described [1-8]. Following incubation of allergen coated wells with an appropriately diluted serum sample, allergen-specific IgE is detected using a secondary antibody mixture of biotinylated monoclonal anti-IgE antibodies, streptavidin alkaline phosphatase as the enzyme conjugate, and p-nitrophenylphosphate (pNPP) as substrate reagent. Specific IgE reactivity to the allergens is then estimated by determining the absorbance of each well measured at 405 nM using an automated plate reader. All results are expressed as ELISA Absorbance Units (EAU), which are background-corrected observed responses expressed as milli absorbance [1].

Statistics

A coefficient of variation was calculated as the ratio of standard deviation and means of the responses observed for the calibrator solutions within different runs in multiple laboratories. Pearson’s correlation statistic was used for inter-laboratory comparison among individual allergens. Statistical analyses were conducted using EXCEL (2016; Microsoft; Redmond, WA, USA).

Results

The assay variance (% CV) observed with the calibrator solutions in the different laboratories are presented in Table 1 and are representative of the assay reproducibility in the various laboratories. The average intra-assay % CV among positive calibrators (#1-5) was 7.3% (range=2.8%-17.6%); differences among laboratories or between assays and within assay runs were not detected. No substantial difference in results among various operators were revealed. The average inter-operator variance documented for Stallergenes Greer technicians was calculated to be 7.9% (range=6.5%-9.0%). Increased intra-assay variability was evident with the background ODs (average 9.2%; range 4.8%-22.5%). A negative response is classified as anything with an EAU below 150 [1]. Any analysis of results below this threshold, especially when looking at %CV and relative differences, should be done cautiously.

Table 1: Calculated variance of macELISA calibrator solutions observed with different laboratory runs by multiple operators during the 2021 Proficiency evaluation.

table 1

*Calibrator #1 is prepared as a dilution of a sera pool which is highly reactive to mite allergens; Calibrators #2 – #5 are prepared as a serial 3-fold dilution of calibrator #1.
†Background responses observed with diluent in place of serum sample.

To evaluate the strength of association with the magnitude of EAU results observed for each allergen among the different laboratories   a Pearson’s correlation coefficient was determined (Microsoft Excel 2016) for each laboratory pair. Because the macELISA is designed  to yield comparable responses in dog and cat samples, comparison  of results among affiliate laboratories included both cat and dog samples as a single population of sera samples [5-8]. These results (Table 2) demonstrate that good inter-laboratory correlation (r>0.80; p<0.001) is evident between the results observed in Stallergenes Greer laboratory and those observed in six affiliate laboratories for all pollen, mite, and fungi allergens. The overall correlation of results observed in the various laboratories are summarized in Table 3; a very strong correlation (r>0.90, p<0.001) was demonstrated between and among the results of the participating laboratories.

Table 2: Inter-laboratory correlation of macELISA results observed with individual allergens in Stallergenes Greer Laboratory and the results observed in the individual affiliate laboratories.

table 2

*Pearson Correlation Coefficient (r); Good Correlation (r > 0.8, p<0.001)

Table 3: Inter-laboratory correlation of macELISA results observed among individual affiliate laboratories

table 3

*Pearson Correlation Coefficient (r); Good Correlation (r > 0.8, p<0.001)

For an evaluation of the dose response in this ELISA, a five tube three-fold serial dilution of a reactive dog sera pool was prepared using a negative cat sera pool as diluent. Each of the dilutions was then evaluated by all of the participating laboratories as unknown independent samples. Similar responses were yielded by all of the laboratories and the results observed within the various laboratories are encompassed by the acceptable variance limits [1-3] (±20%) established for macELISA. Further, the magnitude of responses observed in each laboratory was reduced in direct proportion to dilution. Consequently, the dose responses for the individual allergens are presented as aggregate responses. The results presented in Figure 1 confirm the sera pool was highly reactive to mites as well as grass, weed, and tree pollen allergens, but it was not reactive to fungi. To be expected, the magnitude of responses observed in each laboratory was reduced in direct proportion to dilution. Results from the final tube in the dilution scheme yielded results that were indistinguishable from negative responses, indicating a dilution extinction of detectable response [9-14].

References

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  2. Lee KW, Blankenship KD, McCurry ZM, Kern G, et al. (2012) Reproducibility of a Monoclonal Antibody Cocktail Based ELISA for Detection of Allergen Specific IgE in Dogs: Proficiency Monitoring of macELISA in Six US and European Vet. Immunol Immunopathol 148: 267-275. [crossref]
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  14. Tijssen P (1993) Processing of data and reporting of results of enzyme In: Burdon, RH, van Knippenberg PH, editors. Practice and Theory of Enzyme Immunoassays 385-421. Elsevier, Amsterdam.

Higher Awareness of the Need for the Education in Medical Mediation Practitioners in Hospitals in Japan

DOI: 10.31038/JCRM.2022564

Abstract

Overcoming the negative feelings of patients and their families toward healthcare professionals is critical to resolving medical accident disputes in Japan. To address this issue, a medical mediation model for conflict resolution has been developed and training has been provided. However, it is not studied how those who have completed the training perceive the model in the medical field. Therefore, we conducted a survey on this point. Fifty consenting participants from across Japan were asked to answer 13 questions. Each item was rated on a scale of 1 (no need at all) to 10 (need is essential). At the same time, respondents were asked to indicate whether or not they practice medical mediation. The group that answered that they were practicing was divided into “P” group (n=28) and the group that answered that they were not practicing into “No P” group (n=21), and the Wilcoxon test was used to compare the evaluation scores. In question 2 (Satisfaction with medical mediation education), the P group was 4.68 (mean) ± 2.29 (standard deviation) compared to 3.24 ± 1.81 for the No P group, a significant difference. In the other items, both groups scored 6 or more points. The items with higher scores in the P group and significant differences were six items. They were informed consent support, cognitive conflict resolution, and need for mediation education. These results show that there are differences in perceptions of medical mediation between practitioners and non-practitioners of medical mediation. The need for medical mediation education was also inferred. The study suggests the need to ensure the quality of medical mediation by providing continuing medical mediation education.

Keywords

Medical mediation, Necessity, Awareness, Education, Practitioner

Introduction

Mediation is used in a wide range of fields, including justice and education, as a means of conflict management [1]. However, it is not widely used in medicine. The reason for this is that life, death, and physical disabilities inevitably occur during medical treatment, and the accompanying loss of trust and anger strongly dominate the minds of patients and their families, making it difficult for healthcare providers to deal with such situations. For this reason, various mediation education and training programs have been conducted in healthcare [2,3]. We have developed a “medical mediation” model for the purpose of addressing this issue in the literature [4]. This model is defined as follows. It is a relationship adjustment model that supports the prevention and adjustment of cognitive discrepancies (Cognitive Imperfection) by promoting information sharing through facilitating dialogue between the patient and the healthcare providers. In other words, medical mediation is a model for consensus building based on respect for autonomy and collaborative decision-making [5].

Based on this model, we have developed a two-day training program, an educational program consisting of theory, skills learning, and role-playing [5], and have conducted it with Japanese medical professionals for more than a decade [6,7]. As a result, the program has reduced the number of court cases involving medical disputes, improved communication between medical personnel and patients as well as between professionals, provided support for informed consent, and improved the quality of medical safety [8,9]. On the other hand, after the completion of this training program, the study of medical mediation is still left to the independent matter of each participant. Therefore, continuous training of trainees is necessary to maintain the practical and theoretical quality of medical mediation, but the status of awareness of medical mediation among trainees who have completed the training is unknown. The purpose of this study was to clarify how training completers understand medical mediation while working in the medical field.

Methods

In conducting the study, it was anticipated that there would be differences in perception of the specific content and necessity of education depending on whether or not the respondents were practicing as medical mediators after completing the training.

Working Hypothesis

Practitioners and non-practitioners do not differ in their perception of and need for medical mediation education.

Exploratory Survey Period

August 31, 2021 to September 30, 2021

Target

Hospitals with training completers throughout Japan were asked to participate. Among them, 20 facilities were selected from among those that had obtained consent for this survey from physicians at the assistant director level or above and assistant nursing directors or above at facilities that had obtained cooperation for this survey. The questionnaire was then sent and collected directly to 50 medical mediators working in the medical field at those facilities. The subjects to whom the questionnaire sheets were sent were those who fulfilled the following conditions training completers who had attended a two-day basic medical mediation training course at least one year in the past.

Questionnaire

A 13-item self-administered questionnaire was distributed.

Respondents were asked to indicate whether or not they had practiced medical mediation to date.

Next the respondents were asked to rate their responses to each item on a 10-point scale from 1 (not at all approve) to 10 (fully approve).

Q1: Awareness of medical mediation

Q2: Satisfaction with medical mediation education

Q3: Change in feelings due to medical mediation education

Q4: Contribution of medical mediation to resolving cognitive conflicts

Q5: Contribution of medical mediation to informed consent

Q6: Contribution of medical mediation to reducing psychological burden on patients and families

Q7: Contribution of medical mediation to psychological burden of health care providers

Q8: Change in the relationship between medical professionals due to medical mediation education

Q9: Contribution of medical mediation to daily medical and nursing care (Q10 and Q11 are open-ended questions to get specific understanding)

Q10: Situations in which medical mediation is applied

(1) Post-accident response, patient consultation, medical consultation

(2) Informed consent

(3) Terminal care and decision-making

(4) Routine medical treatment

(5) All of the above

Q11: Expectations for medical mediation education (free answer)

Q12: Necessity of medical mediation in medical education

Q13: Necessity of medical mediation for medical professional

Data Analysis

Respondents were divided into two groups according to their responses of whether or not they practiced. That is, the group that practiced medical mediation was designed as a Practitioner (P) group, and the group that did not practice as designated as the non-practitioner (No P) group. Descriptive statistics were obtained for Q excluding Q10 and Q11. Next, a Wilcoxon test (rank sum) was performed, and P<0.05 was considered a significant difference between the groups. JMP Ver. 14 by SAS was used for the analysis. Questions 10 and 11 were excluded from the analysis because they were intended for quantitative analysis.

Ethics

Individual consent was obtained from research collaborators and respondents. In consideration of personal information, confidential treatment was performed and researcher ethics were observed.

Results

The valid response rate was 98%. Respondents ranged in age from 30 to 65 years. The breakdown of respondents’ occupations was 25 physicians, 13 nurses, and 11 medical staff (3 medical social workers and 8 clerical staff).

Table 1 shows the descriptive statistics and test results for Q1 through Q13, excluding Q10 and Q11. The scores for Q2 were lower than the scores for the other questions in both the P and No P groups, i.e., lower than 5 points indicating neither satisfaction nor dissatisfaction, indicating a low level of educational satisfaction. Although Q12 and Q13 showed significant differences, the mean differences were smaller (1.1 and 0.68) compared to the mean differences between P group and No P group of 1.44 to 2.43 in Q1 to Q5, which also showed the same significant differences.

On the other hand, there was no significant difference between the P and No P groups in Q6 to Q9. The P group scored more than 7 points, while the No P group also scored more than 6 points, which was higher than the midpoint of 5 points.

Discussion

In the United States, when a medical accident occurs, the legal process begins immediately [10]. In Japan, such a response is considered undesirable due to social and cultural backgrounds. Considering this background, we developed a Japanese “medical mediation” model [11,12]. This model is a conflict management model that focuses on the psychological reactions of Japanese people when a medical accident occurs and their responses to negative feelings toward medical personnel. This study was to clarify how training completers understand medical mediation while working in the medical field.

As shown in Table 1, Q2 indicates that, despite significant differences in satisfaction with this model with regard to educational satisfaction, the overall level of satisfaction was considered low. On the other hand, Q12 and Q13, which asked about the need for education from a broader perspective away from the medical field, showed that respondents in both P and No P groups were strongly aware of the need for such education. These results suggest that there is a need for medical mediators to educate medical professionals and medical students about this model, as well as a need for specific guidelines and their contents when providing education in medical settings.

Table 1: Descriptive Statistics for Questions and P-value for Wilcoxon test

Group

N

Mean

SD

95% CI

P value

Q1

P

28

8.54

1.97

7.77, 9.30

0.0018

No P

21

6.57

2.31

5.52, 7.62

Q2

P

28

4.68

2.29

3.79, 5.57

0.0499

No P

21

3.24

1.81

2.41, 4.06

Q3

P

28

8.5

1.48

7.93, 9.07

0.0018

No P

21

6.19

3.04

4.81, 7.58

Q4

P

28

8.14

1.84

7.43, 8.86

0.0021

No P

21

5.71

2.97

4.36, 7.07

Q5

P

27

8.30

1.75

7.60, 8.99

0.0053

No P

21

6.33

2.71

5.10, 7.57

Q6

P

28

7.86

1.65

7.21, 8.50

0.3875

No P

21

7.52

1.64

6.75, 8.29

Q7

P

28

7.71

1.76

7.03, 8.40

0.3934

No P

21

7.33

1.77

6.53, 8.14

Q8

P

28

7.54

1.86

6.82, 8.26

0.0838

No P

21

6.38

2.42

5.28, 7.48

Q9

P

28

9.07

1.30

6.82, 8.26

0.0555

No P

21

8.14

1.77

5.28, 7.48

Q12

P

27

9.67

1.07

9.24, 10.09

0.0085

No P

21

8.57

2.48

7.44, 9.70

Q13

P

28

9.82

0.48

9.64, 10.01

0.0389

No P

21

9.14

1.39

8.51, 9.77

Group: P: Practitioner, No P: No-practitioner, SD: Standard Deviation, CI: Confidence interval.
The evaluation score was between 1 and 10 points. 1: no need at all. 5: neither. 10: the need is essential.
Q1: Awareness of medical mediation.
Q2: Satisfaction with medical mediation education.
Q3: Change in feelings due to medical mediation education.
Q4: Contribution of medical mediation to resolving cognitive conflicts.
Q5: Contribution of medical mediation to informed consent.
Q6: Contribution of medical mediation to reducing psychological burden on patients and families.
Q7: Contribution of medical mediation to psychological burden of health care providers.
Q8: Change in the relationship between medical professionals due to medical mediation education.
Q9: Contribution of medical mediation to daily medical and nursing care.
Q12: Necessity of medical mediation in medical education for physicians.
Q13: Necessity of medical mediation education for medical professionals.

Significant differences in mean values were found between the P and No P groups for Q1 through Q5, which reflect specific situations in which medical mediators experience the evaluation of medical mediators. On the other hand, no significant difference was found in the observational evaluation items, Q6 to Q9, which were slightly removed from the medical mediator’s own experience. It was considered possible that significant differences could be found somewhere in Q6 to Q9 from the practice of medical mediations. The reasons for the lack of differences may be that the questions were not specific enough, or that the emphasis was placed on the results, which may have resulted in a slight psychological burden or a change in the relationship that was not noticed.

The scores of the No P group in Q6 to Q9, where no significant differences were also found, showed more than 6 points. This suggests that the psychological burden on patients/families and health care providers, the relationship between health care providers, and the possibility of contribution to daily medical treatment and nursing care are seen in the medical mediation model.

In this study, the decision of whether or not to practice medical mediation was made by the respondents themselves, a subjective judgment. It is assumed that this influenced the results. It would have been more appropriate to clarify the distinction between practicing and non-practicing and to ask respondents to answer each question.

The remaining 30% of the No P group thought that medical mediation would not be used at the end of life. The results of this study suggest that the usefulness and necessity of medical mediation can only be realized when it is actually used in medical practice. Even if the participants understood the necessity of medical mediation, there was a difference in their perception of its suitability for practical use. In addition, the study population was small, and further study with a larger number of subjects is needed.

This study revealed that awareness of medical mediation and evaluation of the necessity of medical mediation was high among practicing medical mediators. The study also suggested the possibility of a medical mediation model as conflict management that includes psychological content in the medical field.

Conclusion

Practitioners and non-practitioners differed in their perception and need for medical mediation education. Continuous education and training for those who have completed training is necessary. The content of the training should focus on specific issues faced in the field.

Acknowledgments

We would like to thank all parties involved in the survey for their cooperation.

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